protein stability and folding

... energetics of protein folding/ unfolding transitions (II, 17) and a protein- hormone interaction (74). As noted above, the thermodynamics are related to the buried polar and apolar surface areas and the ... modeling protein folding intermediates (I 7,76), protein- ligand complexes, and novel protein structures. Third, it can serve as a diagnos- tic approach for assessi...

Ngày tải lên: 11/04/2014, 10:13

... studied the structure, function, stability and folding of the four-helix bundle protein rop. Rop is an excellent model system for under- standing protein structure and stability: it can be expressed in ... characterized, and all were helical, mono- meric and stable. NOESY, 15 N- 1 H HSQC and 13 C- 1 H HSQC NMR spectra indicated that four of the five proteins had well-ordered...

Ngày tải lên: 19/02/2014, 12:20

... improves stability and folding in hyperthermophilic disulfide-containing proteins and could provide useful information with respect to engineering stable proteins and enzymes for therapeutic and industrial ... hyperthermophilic Archaea require a CXC motif for stability and folding Giovanna Cacciapuoti, Iolanda Peluso, Francesca Fuccio and Marina Porcelli Department of Bioch...

Ngày tải lên: 07/03/2014, 00:20

... thermodynamic stability (DG U ), and kinetic folding ⁄ unfolding parameters (k f and k u ) for hyperthermostable proteins. If not otherwise stated, T M and DG U refer to pH 7, and k f ⁄ k u to pH 7 and ... kinetic limit to protein thermal stability: a proposal regarding protein stability in vivo and its rela- tion with misfolding diseases. Protein Struct Funct Genet 4...

Ngày tải lên: 07/03/2014, 05:20

... Fersht, A.R. (1991) Protein engineering in analysis of protein folding pathways and stability. Methods Enzymol. 202, 8 2–112. Ó FEBS 2004 Proline residues in ubiquitin stability and folding (Eur. J. ... lnk unfold  þð1 À b f ÞDDG=RT ð7Þ where k fold ° and k unfold ° are the rate constants for folding and unfolding of WT*, k fold and k unfold are the folding and unf...

Ngày tải lên: 07/03/2014, 16:20

... misfolded proteins [5–8] and remove protein aggregation [9–12]. Mechanisms of protein osmolyte interactions, the effect of osmolytes on protein stability, and how osmo- lytes correct protein misfolding ... effects that urea has on protein function [16–19]. Despite significant advances in understanding the effect of osmolytes on protein stability, folding and the activity...

Ngày tải lên: 07/03/2014, 00:20

... with the thermostability of its tetrameric and monomeric structures. Thermostability and protein structures We have analyzed the structures of hTTR (Protein Data Bank code: 1F41 [12]), sbTTR (Protein ... specificity and thermostability. Proteins 37, 619–627. 48 Wang M, Wales TE & Fitzgerald MC (2006) Conserved thermodynamic contributions of backbone hydrogen Stability and fibr...

Ngày tải lên: 16/03/2014, 01:20

... unspecific cleavage and unassigned modifications. Protein identifica- tion and modification returned from mascot were manually examined and filtered to create a confirmed protein identifi- cation and modification ... [51], and refinement and model building were carried out iteratively using refmac [52], phenix. refine [53,54] and coot [55]. The coordinates and structure factors wer...

Ngày tải lên: 16/03/2014, 06:20

... features of proteins of the GH72 family and d emonstrate that the most N-terminal highly conserved cysteine is crucial for the folding and maturation of Gas1p. Materials and methods Strains and growth ... biochemical and structural characterization of the protein as a r epresentative of the GH72 family. Spectroscopic analyses o f t he purified proteins were performed, and the b...

Ngày tải lên: 16/03/2014, 18:20

... purple acid phos- phatases and methionyl aminopeptidases [6–8]. In vitro refolding Wild-type AMPP and AMPP#3-22 were overexpressed and purified. Subsequently, the purified proteins were denatured ... metals. Metalloproteins can fold via metal-dependent or metal-independent pathways [20,21]. They may bind metal ions before polypeptide folding, after complete protein folding, or after p...

Ngày tải lên: 23/03/2014, 07:20