protein arrays, methods and protocols
protein arrays, methods and protocols
... throughput, and automation. Array- based methods for protein analysis afford a high-throughput format by which to screen protein protein, protein DNA, and protein small molecule interactions and provides important ... transfer vector design and ligation of cDNA inserts, (c) transfection and viral amplification, (d) deep-well viral amplification and protein expression, an...
Ngày tải lên: 11/04/2014, 10:11
g protein signaling, methods and protocols
... 237 Methods and Protocols Edited by Alan V. Smrcka Methods and Protocols G Protein Signaling G Protein Subunit Purification from Sf9 Cells 21 21 From: Methods in Molecular Biology, vol. 237: G Protein ... recombinant G i α and G o α proteins from Escherichia coli. Methods Enzymol. 195, 202–215. Methods in Molecular Biology TM Methods in Molecular Biology TM Edited...
Ngày tải lên: 10/04/2014, 22:24
Glycoprotein Methods and Protocols - P1
... Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P2
... 7.4, with 0.1% CaCl 2 at 37°C Histological Methods for Detection of Mucin 29 29 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histoch...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P3
... mucins, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... the biosynthesis and secretion of mucin, which is discussed in Subheadings 3.2. and 3.3. 1. Measure protein concentrations according to the BCA protein assay and calculate the protein concentration ... NJ 6 Quan...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P6
... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-form...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolu...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P8
... 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., Totowa, NJ 10 Amino Acid Analysis of Mucins Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a black residue. This residue appears to precipitate protein/ amino acids, interferes with 114 Yan a...
Ngày tải lên: 08/10/2012, 10:25