protein arrays, methods and protocols
protein arrays, methods and protocols
... throughput, and automation. Array-
based methods for protein analysis afford a high-throughput format by which to screen
protein protein, protein DNA, and protein small molecule interactions and provides
important ... transfer vector design and ligation of cDNA inserts, (c) transfection and
viral amplification, (d) deep-well viral amplification and protein expression, an...
g protein signaling, methods and protocols
... 237
Methods and Protocols
Edited by
Alan V. Smrcka
Methods and Protocols
G Protein
Signaling
G Protein Subunit Purification from Sf9 Cells 21
21
From:
Methods in Molecular Biology, vol. 237: G Protein ... recombinant G
i
α and G
o
α proteins from Escherichia coli. Methods Enzymol. 195, 202–215.
Methods in Molecular Biology
TM
Methods in Molecular Biology
TM
Edited...
Glycoprotein Methods and Protocols - P1
... Methods in Molecular Biology
TM
HUMANA PRESS
Glycoprotein
Methods
and Protocols
Edited by
Anthony P. Corfield
VOLUME 125
The Mucins
Methods in Molecular Biology
TM
HUMANA ... Mucins
Glycoprotein
Methods
and Protocols
Isolation of Large Gel-Forming Mucins 11
Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu-
cins were mixed with DNA and...
Glycoprotein Methods and Protocols - P2
... 7.4, with 0.1% CaCl
2
at 37°C
Histological Methods for Detection of Mucin 29
29
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... nature and
limitations of the individual techniques, and (2) interpretation and reporting of mucin
staining.
1.1. Nature and Limitations of Mucin-Staining Methods
Mucin histoch...
Glycoprotein Methods and Protocols - P3
... mucins, in Glycoprotein
Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.
3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and
Protocols: The ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi-
cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The...
Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 57
57
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... poly-
L
-lysine for 5 min
and dried at room temperature.
3. Methods
3.1. Observation and Measurement
of Mucous Thickness on Unfixed Mucosal Sections (
see
Notes 1–4)
This is a rapid and simple method...
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... the biosynthesis and secretion of mucin, which is discussed in Subheadings
3.2. and 3.3.
1. Measure protein concentrations according to the BCA protein assay and calculate the
protein concentration ... NJ
6
Quan...
Glycoprotein Methods and Protocols - P6
... few separation techniques available to use with these
77
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... small gel, use 1.6 g of
agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of
buffer.
2. Leave to cool before pouring (hand hot) and insert well-form...
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87
87
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin
populations with regard to mass and size utilizing rate zonal centrifugation and electron
microscopy. Other methods for the absolu...
Glycoprotein Methods and Protocols - P8
... 113
113
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., Totowa, NJ
10
Amino Acid Analysis of Mucins
Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a
black residue. This residue appears to precipitate protein/ amino acids, interferes with
114 Yan a...