protein and peptide analysis by mass spectrometry

... L., and McLuckey, S. A. (1988) Mass Spectrometry /Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry VCH, New York. 16. Hogeland, K. E , Jr and Demzer, M L (1994) Mass ... 1 Mass Spectrometry in the Analysis of Peptides and Proteins, Past and Present Peter Roepstorff When the editor, John Chapman, asked me to write the introductory chap...

Ngày tải lên: 11/04/2014, 10:11

... binding (T46, A55, T69 and R85) or RNA cleavage (R73, D75 and H17) were analysed by mass spectrometry in RNA binding and cleavage assays. A pair of substrates, 5¢-AUACA-3¢, and its uncleavable mimetic ... residues of the protein and by analy- sing in vitro their effects on RNA binding and RNA cleavage using short RNA substrates and native MS assays. Our analysis focu...

Ngày tải lên: 16/03/2014, 03:20

... dithio- threitol and iodoacetamide, with quantification of Cys by detection of mass change of a peptide after reduc- tion and alkylation. These and other approaches have been described previously [19], and ... protease, and the pep- tides subjected to MS. The peptide mass fingerprint from MALDI-TOF MS showed m ⁄ z values corres- ponding to all eight theoretical peptides plus...

Ngày tải lên: 19/02/2014, 05:20

... [14a- 1 H 2 ]methylene-H 4 MPT was prepared by spontaneous reaction of H 4 MPT and 1 H 2 CO and [14a- 2 H 2 ]methylene-H 4 MPT by spontaneous reaction of H 4 MPT and 2 H 2 CO [30]. FprA from M. marbur- gensis [20] and Mtd from ... data were carried out in excel 2000 and transformed into stick spectra separated by 1 Da [31]. Acknowledgements This work was supported by the Max...

Ngày tải lên: 20/02/2014, 03:20

... two alternative protein standards, bovine serum albumin and ovalbumin, are 0.66 and 0.75, respectively. 3. Plastic and glassware used in the assay should be absolutely clean and detergent-free. ... the Coomassie blue protein assay to different proteins. Anal. Btochem. 184, 111-I 16. 6. Spector, T. (1978) Refinement of the Coomassie blue method of protein quan- titation. A...

Ngày tải lên: 10/04/2014, 11:03

... overall peptide function, as has been shown by the hypothalamic releasing factor binding to its receptor [2], or by the amyloid b -peptide and the implications in senile plaque formation and pathogenesis ... were incubated at 37 °C, 50 °Cand70°C, respectively. From these, aliquots of 2 lL were taken at 0, 1, 2, 4, 6 and 24 h, and prepared for MS analysis as described above 9...

Ngày tải lên: 19/02/2014, 12:20

... oligosaccharides, were initially elucidated by high- sensitivity ES-MS/MS analysis, and then their full structure assignment was completed by methylation analysis and 1 H-NMR. Three new structures were ... hepatitis B and HIV tests. Written consent was obtained from this volunteer for analysis of the milk sample. Fat was removed by centrifugation at 4 °C (5000 g,30min )and p...

Ngày tải lên: 19/02/2014, 12:20

... 33 kDa and 28 kDa, respectively, and two additional protein bands at apparent masses of 42 and 190 kDa [9]. For sequence analysis, the BAP31 immunoprecipitation protocol was scaled up and the ... characterization was achieved by manually excluding tandem mass spectra of poor quality, by restricting the database to the proteins that were identified in a first pass approach, and...

Ngày tải lên: 20/02/2014, 23:20

... removal and reproducibility of the system. Figure 4. Cool plasma analysis calibrations in IPA. Standard addition at 0, 5, 10 and 20 ppt, showing effective removal of 12 C 2 and 40 Ar 12 C ... Coupled Plasma Mass Spectrometry (ICP- MS) is used for the routine monitoring of trace and ultra-trace metal contaminants in aqueous-based chemicals. Recent advances in ICP-MS...

Ngày tải lên: 05/03/2014, 21:20

... d 4 (B) is observed. The peptides indicated by arrowheads were identified by exact mass measurement. The peptide labeled by curved arrows was identified by tandem mass spectrometry as a mono-linked ... Chemical cross-linking and mass spec- trometry to map three-dimensional protein structures and protein- protein interactions. Mass Spectrom Rev 25, 663–682. 10 Lutter L...

Ngày tải lên: 15/03/2014, 00:20