oral transmucosal delivery of protein and peptide therapeutics

... laboratory and the structureactivity data collected for the various series contribute to the long-term goals of understanding the mechanism of lipofection, and the rational design of pharmaceutically ... amount of chloroform and loaded onto a silica gel column (26 ã 2.8 cm) The column was eluted sequentially with 100 mL chloroform, and 1, 3, 5, 7, 8, 9, 10, 12 (300 mL) and 15% methanol chloroform ... 1) of the largest size with a mean diameter around lm Use of DOPE and cholesterol to enhance the genedelivery properties of cationic lipids has been extensively documented [1621] For 1,3lb2 and...

Ngày tải lên: 23/03/2014, 07:20

... the loss of viability of vaginal cells and Lactobacillus species [17,18] Page of 13 The present study focuses on a comprehensive assessment of the use of natural and neutralized lime and lemon ... Juice and Household Vinegar are Toxic to Immortalized and Primary Cells, and Toxicity is Reduced by pH Neutralization The antiviral and cytotoxic effects of natural and pH neutralized lemon and ... The pH of freshly squeezed lemon and lime juice ranged from 2.1-2.4 and 2.2-2.3, respectively The pH values of orange and grapefruit juices were 3.3 and 3.0, respectively Apple (pH 3.7) and tomato...

Ngày tải lên: 10/08/2014, 05:21

... analyses showed a progressive loss of intact ETA and ETA-A in the presence of ATP, with concomitant generation of ETA and ETA-A fragments Incubation in the Fig Assessment of ETA-degrading activity associated ... generated at pH (peptides 4a–c) or pH (peptides 6a–d) were subjected to N-terminal sequence analysis (B) Sequences of ETA-A and ETA-B The A and B moieties are connected by both a peptide bond (Arg279-Gly280) ... lane contained 30 lg of cytosolic protein The arrows to the left indicate the mobilities of intact ETA ( 66 kDa) and ETA-A ( 37 kDa) (B) Membrane translocation of toxin peptides in cell-free...

Ngày tải lên: 18/02/2014, 04:20

... beforehand with a primase assay on poly (dT) Lanes and 2, control reaction with DNA polymerase a-primase lacking TAg or vice versa; lanes and 4, 0.2 U and 0.4 U of human; lanes and 6, 0.2 U and ... with BSA as a standard (Biorad, Munich) DNA polymerase a and DNA primase assays were performed as previously described [34,42,43] Preparation of S100 extracts and replication of SV40 and JCV in vitro ... New York, USA 41 Bradford, M (1976) A rapid and sensitive methode for the quantitation of microgram quantities of protein utilicing the principle of protein- dye binding Anal Biochem 72, 248–254...

Ngày tải lên: 17/03/2014, 03:20

... [16] Broad band observed for carboxylic group disappeared in the spectra of complexes indicating deprotonation of ligand In the spectra of compounds V-VIII, appearance of new band of medium intensity ... serum; 25 mM of HEPES, and 0.22 μg of penicillin and streptomycin, respectively at 24°C in an incubator mg of each test compound (I-VIII) was dissolved in mL of water, ethanol, methanol and DMSO ... of Synthesis of antimony complexes Aqueous solution of SbCl3 was made by dissolving 0.5 g (2.19 mM) in 10 mL, and a few drops of dil HCl were added; to this solution, equimolar amount of ligand...

Ngày tải lên: 21/06/2014, 02:20

... 1604 of free radicals and chelation of transition metals, acting both in the initiation step and in the propagation of oxidation [6] The intermediate species formed by the action of antioxidant ... potential use of nanocapsules includes the protection of drugs against inactivation in the gastrointestinal tract [21], delivery of poorly water-soluble compounds [24, 25], and protection of sensitive ... the main objective of our study was to develop aqueous rutin delivery systems and to evaluate the influence of the association of rutin to different nanostructures (nanocapsules and nanoemulsions)...

Ngày tải lên: 21/06/2014, 17:20

... the balance of paternal contribution (El-Kassaby and Ritland, 1986b), improve seed yields (Webber, 1987) and reduce the negative impact of selfing and contamination (El-Kassaby and Ritland, 1986b; ... threat of inferior gametic infiltration into orchard populations is a constant concern and estimated levels of contamination range from 6-56% (Smith and Adams, 1983; El-Kassaby and Ritland, 1986a; ... a minimum of using a chart speed of cm/min The rate of oxygen consumption was calculated using the percent change in volume of dissolved oxygen for a 5-min period and the solubility of oxygen...

Ngày tải lên: 08/08/2014, 23:22

... expressed proteins have not been completely shown as yet [16-22] The analysis of the spatial and temporal aspects of protein phosphorylation is of great interest for the discovery of functions of specific ... placement of the PTM on the sequence [D] The mass and lists of fragment masses for each peptide are scanned against protein sequence databases, resulting in a list of identified peptides and proteins ... Complex, and phosphorylated peptides related to those previously mentioned proteins TiO2 and IMAC allowed the isolation of phoshorylated proteins: HuR RNA binding and p38 MAP Kinase, and phosphopeptide...

Ngày tải lên: 10/08/2014, 09:22

... Briefly, 0,25 ml of cold chloroform was added in order to firstly reach the proportion of 1/2/0.8 CHCl3:MeOH:H2O, and after potent vortex another 0,25 ml of cold chloroform and 0,25 ml of water were ... Bradford MM: A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein- dye binding Anal Biochem 1976, 72:248-254 Page 11 of 11 32 Levi M: ... whole cells, nucleuses and debris in the pellet and the supernatants (homogenates) after protein determination were aliquoted and stored at -80°C until the time of the PAF-CPT and Lyso-PAF-AT assays...

Ngày tải lên: 11/08/2014, 03:20

... Briefly, 0,25 ml of cold chloroform was added in order to firstly reach the proportion of 1/2/0.8 CHCl3:MeOH:H2O, and after potent vortex another 0,25 ml of cold chloroform and 0,25 ml of water were ... Bradford MM: A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein- dye binding Anal Biochem 1976, 72:248-254 Page 11 of 11 32 Levi M: ... whole cells, nucleuses and debris in the pellet and the supernatants (homogenates) after protein determination were aliquoted and stored at -80°C until the time of the PAF-CPT and Lyso-PAF-AT assays...

Ngày tải lên: 11/08/2014, 06:23

... CAp24 tube formation in vitro, and for HIV-1 replication and capsid formation in cultured virus [6] During proteolysis of the Pr55Gag and maturation of CAp24, the NTD of CAp24 refolds into a β-hairpin ... cell (A and B) and viral lysates (C) were then separated by SDS-PAGE, transferred onto a nitrocellulose membrane and detected with a rabbit anti-HIV glycoprotein (A), a pool of anti-CAp24 and anti-calnexin ... (A), rabbit anti-cyclophilin A (B), and anti-calnexin (C) antibodies Positions of specific viral and cellular proteins are indicated on the right number of CAp24 proteins assembled into tubular structures...

Ngày tải lên: 13/08/2014, 05:22

... [10,11,14] and six non structural proteins (NS2, NS3, NS4A/B and NS5A/B) The core protein of HCV forms the nucleocapsid of the virus It binds with RNA and also interacts with numerous cellular proteins ... manner, viral proteins are expressed There are 10 viral proteins: core; envelope protein (E1) and E2 are structural proteins that constitute the virion; a small protein that is essential for protein ... Page of Table indicating HCV Gene and polyprotein sequences submitted in Genbank and their Accession Numbers No HCV Gene and polyprotein Sequences Core Hepatitis C virus isolate PKIS-1 polyprotein...

Ngày tải lên: 14/08/2014, 19:22

... 30 Figure 3.1 Protein expression and purification profile of DENV NS5 FL 65 Figure 3.2 Purification profile of DENV FL NS5 66 Figure 3.3 Purified proteins of DENV FL NS5 67 ... (capsid, membrane protein and envelope) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) (Figure 1.5 C) The conserved 5´ and 3´ UTRs are made up of ~100 and 400 nucleotides ... studies and NITD for enabling me to use their facilities for my research study Special thanks to coordinators of this programme most especially Prof Marcel Tanner of Swiss TPH and Prof Markus Wenk of...

Ngày tải lên: 02/10/2015, 17:14

... Figure Characterization of CuCNPs (A) SEM image of CuCNPs, (B) EDX spectra of CNPs before (left) and after (right) addition of Cu, (C) XPS spectra for CuCNPs (right) and control particles containing ... toxicity of CuCNPs upon reduction of Cu content water, which has a neutral to slightly acidic pH While this feature is promising in terms of the stability of solutions of CuCNPs over long periods of ... effect of competing ligands on Cu release with the idea that the underlying principles governing release could be used in the design/optimization of this class of delivery vectors Release of Cu...

Ngày tải lên: 21/06/2014, 02:20

... contributions MIC and YFH contributed equally to this work MIC and YFH interpreted the data and drafted the manuscript MIC, MW, DC and JTC designed and performed the RNAi and JC virus experiments MZ and GJT ... Journal of Biomedical Science 2010, 17:51 http://www.jbiomedsci.com/content/17/1/51 Page of Figure In vivo effects of IL-10 shRNA on production of IL-10 and TNF-α in BALB/c mice Groups of BALB/c ... by deactivating macrophages and silencing their synthesis of TNFα, IL-6, IL-1α, IL-8, and an array of other proinflammatory cytokines and chemokines In mouse models of toxic shock, IL-10 is produced...

Ngày tải lên: 10/08/2014, 05:21

... integrating TB and HIV care in primary healthcare clinics singled out high service loads at both the TB and HIV entry points, duplication of services and underutilization of staff, and TB and HIV services ... alleviation of health worker shortages; (3) improved HCT training of professional and lay health workers; and (4) community outreach activities Fears of HIV/AIDS, TB-HIV coinfection, death, and stigma ... Page of 10 of their HIV test results, lack of appropriately trained healthcare personnel, limited availability of antiretroviral medications, poor monitoring of patient care, and fragmented delivery...

Ngày tải lên: 10/08/2014, 10:23

... effect of [PDDA] on sizes and zeta-potentials of DODAB BF/AmB/CMC assemblies at mM DODAB, 0.005 mM AmB and mg/mL CMC is on Figure The region of PDDA concentrations for size minimization and high ... in zeta-potential and a decrease of % of cell viability (Table 2) Table summarized the different properties of assemblies at low and high P One should notice that coverage of a drug particle ... stability was very narrow and around mg/ mL PDDA Below and above this concentration, about 300 nm and negative zeta-potentials, or 500–700 nm of zeta-average diameter and positive zeta-potentials...

Ngày tải lên: 11/08/2014, 00:22

... analysis and interpreted results BMW and CP carried out isolation and measurement of neutrophils CK read the manuscript and supported the lab team MvG conceived the study, and critically revised and ... concentrations of DHEA (10-5 M and 10-8 M) As a first important finding, the strongest effects of DHEA occurred with a DHEA concentration of 10-8 M compared to a concentration of 10-5 M A direct effect of ... seem to have an influence on the outcome and severity of complications after trauma and sepsis and are, therefore, interesting candidates for medication and drug targets Previous studies by our...

Ngày tải lên: 13/08/2014, 01:20

... the potential of chitosan to improve the transmucosal delivery of macromolecular drugs The positive effect of chitosan solutions/powders on the transmucosal delivery of peptides and proteins has ... activation of suicidal genes in the bacteria Several studies have reported successful application of bactofection and AGT in genetic vaccination and gene therapy of several tumors, and monogenic and ... The particle size and shape of PEI-based polyplexes depend on the structure and Mw of PEI, the pH and ionic strength of the surrounding environment, the method of preparation and the charge ratio...

Ngày tải lên: 23/08/2015, 17:29