plant functional genomics, methods and protocols

... cells in liquid N 2 and store at –70°C (see Note 17). Plant BAC Library Construction 3 3 From: Methods in Molecular Biology, vol. 236: Plant Functional Genomics: Methods and Protocols Edited by: ... 21 21 From: Methods in Molecular Biology, vol. 236: Plant Functional Genomics: Methods and Protocols Edited by: E. Grotewold © Humana Press, Inc., Totowa, NJ 2 Construc...

Ngày tải lên: 11/04/2014, 10:10

... Contributors Fabrication of cDNA Microarrays 1 1 From: Methods in Molecular Biology: vol. 224: Functional Genomics: Methods and Protocols Edited by: M. J. Brownstein and A. Khodursky © Humana Press Inc., Totowa, ... 548–556. Nylon cDNA Expression Arrays 9 9 From: Methods in Molecular Biology: vol. 224: Functional Genomics: Methods and Protocols Edited by: M. J. Brownstei...

Ngày tải lên: 11/04/2014, 00:52

... Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...

Ngày tải lên: 08/10/2012, 10:25

... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The M...

Ngày tải lên: 08/10/2012, 10:25

... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... important advantage that con- tinuity and thickness of the layer can be directly related to functional efficacy (1,2). Routine histological methods for fixing and staining of mucosal sections, although...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager....

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Human...

Ngày tải lên: 08/10/2012, 10:25

... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolu...

Ngày tải lên: 08/10/2012, 10:25

... 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., Totowa, NJ 10 Amino Acid Analysis of Mucins Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a black residue. This residue appears to precipitate protein/amino acids, interferes with 114 Yan and...

Ngày tải lên: 08/10/2012, 10:25