peptide synthesis protocols

... solid-phase peptide synthesis. J. Am. Chem. Sot. 94, 3102-3106 9 Barany, G., Knetb-Cordomer, N., and Mullen, D. G (1987) Solid-phase peptide synthesis: a silver anniversary report. Int. J. Peptide ... solid phase peptide synthesis. Int. J. Peptide Protein Res. 31,86-97. 3 1. Forest, M. and Fournier, A (1990) BOP reagent for the coupling of pGlu and Boc- His(Tos) in solid p...

Ngày tải lên: 11/04/2014, 10:02

... nonribosomal peptide synthesis; nonribosomal peptide synthetases; peptide synthetases; condensation domain; chloramphenicolacetyltransferase. A broad range of organisms utilize nonribosomal peptide synthesis ... selectivity in nonribos- omal peptide synthesis. Science 284, 486±489. 7. Linne, U. & Marahiel, M.A. (2000) Control of directionality in nonribosomal peptide synt...

Ngày tải lên: 17/03/2014, 11:20

... one structural residue in a nonribo- somal peptide synthetase TEII. Keywords: catalytic triad; fatty acid synthases; nonribosomal peptide synthesis; peptide synthetases; type II thioesterase polyketide ... (4¢-Ppant) metabolic processes, such as the synthesis of fatty acids, polyketides, or nonribosomal peptides. Many polyketides and nonribosomal polypeptides produced by bacteria and...

Ngày tải lên: 30/03/2014, 13:20

... Resin for Synthesis of Peptide Amides Using Boc/Bzl Chemistry 145 5.19 Resins and Linkers for Synthesis of Peptides Using Fmoc/tBu Chemistry 146 5.20 Resins and Linkers for Synthesis of Peptide ... and Methods for Solid-Phase Synthesis 142 5.16 Merrifield Resin for Synthesis of Peptides Using Boc/Bzl Chemistry 143 5.17 Phenylacetamidomethyl Resin for Synthesis of Peptides Usi...

Ngày tải lên: 03/04/2014, 12:14

... 1/12/05, 1:51 PM10 Modern Peptide Synthesis 1 I COMMON STRATEGIES 2 Amblard et al. Modern Peptide Synthesis 3 3 From: Methods in Molecular Biology, vol. 298: Peptide Synthesis and Applications Edited ... the precipitated peptide on the frit three times with MTBE. Peptide Synthesis and Applications Howl_FM_Final 1/12/05, 1:51 PM1 Modern Peptide Synthesis 19 9. Solubilize...

Ngày tải lên: 03/04/2014, 12:15

... previous sections. 2.8. Synthesis of a Mutational Analysis Peptide synthesis chemicals of the previous sections. 2.9. Synthesis of a Peptide Scanning Library Peptide synthesis chemicals of ... Garthersburg, MD). 3. Methods 3.1. Library Synthesis For general procedures on solid phase peptide synthesis, readers are referred to (16) and (17). The peptide mixtures makin...

Ngày tải lên: 11/04/2014, 09:38

... of the peptide mixtures give molecular-weight-indicative ion signals for inter- and intramolecular disulfide-bonded peptides. To enhance the ion signals of the reduced forms of the peptide, ... peptide with the hydrophobrc surface of the stationary phase, it is necessary to reduce the polar charac- ter of the peptide and eliminate any hydrophilic interactions between matrix and...

Ngày tải lên: 11/04/2014, 10:02

... Technical University of Munich, Freising-Weihenstephan, Germany The peptide transporters peptide cotransporter 1 (PEPT1) (SLC15A1) and peptide cotransporter 2 (PEPT2) (SLC15A2) are presently under ... standard procedures in peptide chemistry. The interaction of Bip-Pro with H + ⁄ peptide cotransporters was determined in intestinal Caco-2 cells constitutively expressing human H + ⁄...

Ngày tải lên: 07/03/2014, 05:20

... is mediated by grainy head. Science 308, 381–385. 19 Hayakawa Y (1995) Growth-blocking peptide: an insect biogenic peptide that prevents the onset of metamor- phosis. J Insect Physiol 41, 1–6. 20 Noguchi ... Distribution of growth-blocking peptide in the insect central nervous tissue. Cell Tissue Res 300, 459–464. Y. Ninomiya and Y. Hayakawa Regulation of melanin synthesis in insect cu...

Ngày tải lên: 16/03/2014, 11:20