pcr detection of microbial pathogens

... RER . NAT . Joachim Frey, P h D PCR Detection of Microbial Pathogens HUMANA PRESS Methods in Molecular Biology TM VOLUME 216 PCR Detection of Microbial Pathogens Ta q Ta q Ta q Edited by Konrad ... amplification; and (iv) detection of PCR products (Fig. 1). Pre -PCR pro- cessing comprises all steps prior to the detection of PCR products. Thus, pre- PCR pr...

Ngày tải lên: 11/04/2014, 10:01

... of a combination of 10 PCR primers to be used in a single high-sensitivity mixed PCR reaction for the detection of dengue virus. Pri- mer sequences were computed such that their probability of ... 2011) doi:10.1111/j.1742-4658.2011.08091.x PCR detection of viral pathogens is extremely useful, but suffers from the challenge of detecting the many variant strains of a g...

Ngày tải lên: 14/02/2014, 19:20

... large inventory of normalized mathematical indexes of abnormality. It is the pattern of these mathematical indexes of abnormality, ob- tained from analysis of multiple cardiac cycles of the resting ... (0.86-0.903).(Figure 2). The coordinates of the curve confirmed that a cut-off score of 4.0 pro- vides the best combination of sensitivity and specific- ity for the predict...

Ngày tải lên: 03/11/2012, 10:58

... 8-OH-Gua Introduction Genotoxicity of carcinogens is considered one of the most important contributors to cancer and a marker of the cancer risk of various populations. There are a number of short-term genotoxicity ... YG1042 strain is quite useful for the efficient detection of genotoxic chemicals in the environment. Materials and methods 255 chemicals of a wide variety of...

Ngày tải lên: 05/09/2013, 08:40

... biofilms). In this paper, we will, therefore, address the great potential of the combined use of the current FISH technique and microelectrodes to study the microbial ecology of complex microbial ... extraction RT -PCR PCR DGGE, TGGE or t-RFLP Cloning PCR Sequencing of clones Sequencing of individual bands Comparative sequence analysis Hybridization analysis Dot blot / South...

Ngày tải lên: 05/09/2013, 08:40

... Gene, Tokyo). Detection of pathogens and fecal indicators by PCR and qPCR methods The concentration of (c)DNA of Poliovirus and Adenovirus was determined using real-time qPCR methods with ... indicator bacteria which indicate the presence of pathogens. The concentrations of fecal indicator bacteria were shown to be much higher in different kinds of sediments than thos...

Ngày tải lên: 05/09/2013, 10:15

... may result to the development of oseltamivir-resistant influenza viruses. Furthermore, Japan is at the top of the list of countries for per capita consumption of Oseltamivir (Soderstrom et ... 200.06, respectively. Quantifications of OP, OC and OC-D 3 were based on calibration curves from 0 to 1000 ng/L. The limit of detection (LOD) and limit of quantification (LOQ) value...

Ngày tải lên: 05/09/2013, 10:15

... Simulation of Microbial Depolymerization Processes of Xenobiotic Polymers 12.5 Discussion The degradation rate λ ()M of the exogenous depolymerization model is the ratio of the total weight of ... Simulation of Microbial Depolymerization Processes of Xenobiotic Polymers Figure 12.7 The weight distribution of PEG before and after 5 - day cultivation, and the tran...

Ngày tải lên: 25/10/2013, 08:20

Ngày tải lên: 26/10/2013, 17:15

... the combination of bands 5, 4 and 1 which is of great efficacy for the aims of our analysis. In Figures 1 and 2 the set of bands 5, 4 and 1 are depicted respectively for the month of May 1994 and ... algorithms for the detection of waste areas using LANDSAT TM images. It is worth of noting that the 30 meters spatial resolution of the Landsat-TM sensor makes the process of de...

Ngày tải lên: 16/01/2014, 16:33