molecular genetics of mammalian cells

molecular genetics of mammalian cells

... properties of growing $49 cells. (1) The ability of cAMP to arrest wild-type but not cAMP-resistant mutant cells in the Gt phase of the cell cycle. (2) The extreme sensitivity of cells to white ... against losing cells that have been defrosted is to freeze them in 10% glycerol. Although survival of cells frozen in glycerol is not as good as that of cells frozen in...

Ngày tải lên : 11/04/2014, 09:52

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Báo cáo khoa học: Recent insights into cerebral cavernous malformations: the molecular genetics of CCM pot

... a complete loss, within affected cells, of the two alleles of a given CCM gene. Loss of one of the alleles (ﬁrst hit) would be the result of a germline mutation and loss of the second allele (second ... sequencing of lesion DNA, emphasizing the lack of sensitivity of direct sequencing of lesion DNA. These data established the existence of biallelic somatic and germline...

Ngày tải lên : 15/03/2014, 10:20

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Báo cáo Y học: Growth inhibition of mammalian cells by eosinophil cationic protein pptx

... A431 cells, MDA-MB-453 cells, HL-60 cells and K562 cells were esti- matedtobe% 1±5 l M . ECP signi®cantly suppressed the size of colonies of A431 cells, and decreased K562 cells in G 1 /G 0 phase. ... inhibition of K562 cells in Fig. 2 was shown after 7 days of treatment with ECP, which was Fig. 2. Suppression of cell growth in the presence of ECP. Left, the percentage...

Ngày tải lên : 17/03/2014, 17:20

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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... stimulation of ERK1 ⁄ 2 and Pak1 pathways, geﬁtinib might lead to inhibition of invasiveness of human cancer cells through the inhibition of ERK1 ⁄ 2 and Pak1. The use of geﬁtinib in cells with ... tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors Kenji Takeuchi and Fumiaki Ito Department of Biochemistry...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents pptx

... [1,2]. However, the molecular targets of SQAGs are ambiguous. The aim of the present study was to identify molecular targets that could be of signiﬁcance. Earlier, effects of SQAGs were found ... in the chemical synthesis of a number of forms. We have found a-anomers that possess potent antitumor activ- ity but do not have many of the serious side-effects of Keywords mamm...

Ngày tải lên : 19/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows speciﬁc detection of antigen in mammalian cells pdf

... by FACS of living cells expressing low levels of antigen. Discussion The potential of scFvs for demonstrating the presence of targeted protein antigens in the cytoplasm of mammalian cells is ... significant difference of number of highly fluorescent cells in these cell samples as evidenced by the amount of cell counts in the 10 2 )10 4 range of fluorescence intensity. (...

Ngày tải lên : 19/02/2014, 18:20

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Báo cáo khoa học: Functional characterization of ecdysone receptor gene switches in mammalian cells docx

... 1.647 nm (confidence interval of )0.5963 to 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28), respectively. These ... Discussion The most significant contributions of this study are better understanding of the molecular mechanism of EcR gene switches in mammalian cells and develop-...

Ngày tải lên : 16/03/2014, 12:20

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Báo cáo khoa học: Site-speciﬁc phosphorylation of MCM4 during the cell cycle in mammalian cells pot

... Linearity of DAPI-stained DNA content of a nuc- leus was preserved throughout such treatments; i.e. the DNA content of G 2 , M cells are almost double that of G 1 cells and the DNA content of S cells ... re-replication of DNA in a single cell cycle. Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7]. Targets of the kin...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Identiﬁcation of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells pptx

... transcription of hsp genes and the accumu- lation of Hsps in various mammalian cells and a concomi- tant increase of thermoresistance of cells. Thus, SA may be used for the protection of cells against ... such as Hsp105a and Hsp70 in various mammalian cells. Enhancement of thermoresistance of cells by SA Upon exposure to a sublethal heat treatment, mammalian cell...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo Y học: Molecular cloning of columbamine O-methyltransferase from cultured Coptis japonica cells pot

... (R)- and (S)-coclaurine were the gift of Dr Nagakura of Kobe Women’s College of Phar- macy, and (S)-reticuline was a gift from Dr Facchini of the University of Calgary. The other alkaloids were ... of columbamine was approximately 8.4. Whereas product inhibition of 6OMT and SMT has been reported [8,17], berberine, one of the end-products of isoquinoline alkaloid biosynthesis...

Ngày tải lên : 17/03/2014, 10:20

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