meningococcal vaccines, methods and protocols

meningococcal vaccines, methods and protocols

meningococcal vaccines, methods and protocols

... outer Humana Press Meningococcal Vaccines Edited by Andrew J. Pollard, MD, PhD Martin C. J. Maiden, PhD Methods and Protocols Humana Press Meningococcal Vaccines Edited by Andrew J. Pollard, ... 23 23 From: Methods in Molecular Medicine, vol. 66: Meningococcal Vaccines: Methods and Protocols Edited by: A. J. Pollard and M. C. J. Maiden © Humana Press Inc., Totowa, NJ 2 I...

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dna vaccines, methods and protocols

dna vaccines, methods and protocols

... 2687–2692. 23 From: Methods in Molecular Medicine, vol. 29, DNA Vaccines: Methods and Protocols Edited by: D. B. Lowrie and R. G. Whalen Humana Press Inc., Totowa, NJ 3 Development and Characterization of ... perspective. J. Pharm. Sci. 8, 130–146. 11 From: Methods in Molecular Medicine, vol. 29, DNA Vaccines: Methods and Protocols Edited by: D. B. Lowrie and R. G. Wha...

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meningococcal disease, methods and protocols

meningococcal disease, methods and protocols

... 9 9 From: Methods in Molecular Medicine, vol. 67: Meningococcal Disease: Methods and Protocols Edited by: A. J. Pollard and M. C. J. Maiden © Humana Press Inc., Totowa, NJ 2 Isolation, Culture, and ... Pollard, MD, PhD Martin C. J. Maiden, PhD Methods and Protocols Humana Press Edited by Andrew J. Pollard, MD, PhD Martin C. J. Maiden, PhD Methods and Protocols M E T...

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Glycoprotein Methods and Protocols - P1

Glycoprotein Methods and Protocols - P1

... Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and...

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Glycoprotein Methods and Protocols - P2

Glycoprotein Methods and Protocols - P2

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...

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Glycoprotein Methods and Protocols - P3

Glycoprotein Methods and Protocols - P3

... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The M...

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Glycoprotein Methods and Protocols - P4

Glycoprotein Methods and Protocols - P4

... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method...

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Glycoprotein Methods and Protocols - P5

Glycoprotein Methods and Protocols - P5

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager....

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Glycoprotein Methods and Protocols - P6

Glycoprotein Methods and Protocols - P6

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Human...

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Glycoprotein Methods and Protocols - P7

Glycoprotein Methods and Protocols - P7

... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolu...

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