macromolecular crystallography, part d

... of electron density corresponding to helices and sheets, and build a prelimin- ary model. The theory and operation of both SOLVE and RESOLVE have been described in detail. 1,4,5 In addition, an ... Crystallogr. D 56, 1413 (2000). 44 Z. Dauter, M. Dauter, and E. Dodson, Acta Crystallogr. D 58, 494 (2002). 45 Z. Dauter and M. Dauter, Structure 9, R21 (2001). [1] how bijvoet made the differe...

Ngày tải lên: 11/04/2014, 09:49

... Circular dichroism (CD) arises from the difference of absorption of right- and left- handed polarized light. In protein solutions, far UV CD arises from amide bonds of the peptides and depends on ... negatively charged SDS binds to the denatured protein polypeptide in proportion to polypeptide size. The denatured pro- teins with uniform negatively charged density are then separated in denat...

Ngày tải lên: 22/03/2014, 09:20

... B) Edited by David Barnes and David A. Sirbasku Volume 148. Plant Cell Membranes Edited by Lester Packer and Roland Douce Volume 149. Drug and Enzyme Targeting (Part B) Edited by Ralph Green and ... Methods Edited by Ludwig Brand and Michael L. Johnson Volume 211. DNA Structures (Part A: Synthesis and Physical Analysis of DNA) Edited by David M. J. Lilley and James E. Dahlberg Volume 212. D...

Ngày tải lên: 10/04/2014, 23:13

... David Barnes and David A. Sirbasku Volume 147. Peptide Growth Factors (Part B) Edited by David Barnes and David A. Sirbasku Volume 148. Plant Cell Membranes Edited by Lester Packer and Roland Douce Volume ... XXXIV–LX Edited by Edward A. Dennis and Martha G. Dennis Volume 76. Hemoglobins Edited by Eraldo Antonini,Luigi Rossi-Bernardi, and Emilia Chiancone Volume 77. Detoxication and Drug Met...

Ngày tải lên: 11/04/2014, 10:00

... and thus overcome the drawbacks of the filamentous phage vectors described above. 2°,2°a 2t D. Denhardt, D. Dressier, and D. S. Ray (eds.), "The Single-Stranded DNA Phages." Cold ... supplier. Media, Nutritional Supplements, and Buffers Use distilled water for media and buffers (dH20) and double-distilled water (ddH20) for enzyme reactions. All biochemicals can be purchase...

Ngày tải lên: 11/04/2014, 10:26

... B) Edited by David Barnes and David A. Sirbasku Volume 148. Plant Cell Membranes Edited by Lester Packer and Roland Douce Volume 149. Drug and Enzyme Targeting (Part B) Edited by Ralph Green and ... Enzymes Edited by Gertrude E. Perlmann and Laszlo Lorand Volume XX. Nucleic Acids and Protein Synthesis (Part C) Edited by Kivie Moldave and Lawrence Grossman Volume XXI. Nucleic Acids (Part D...

Ngày tải lên: 11/04/2014, 10:30

... GDP/GDP. At time 0, 1 mM [3H]GDP was added to 20/xM FLAG- RhoA/His6-RhoGDI in nucleotide exchange buffer and incubated at 22 °. Aliquots were taken at the specified times, and MgCI2 was added ... and a cleaved effector protein can be added to the beads at the same time.) Wash the beads three to five times with binding buffer. Add 10 /zl 2× SDS-PAGE sample buffer to the beads, and fractio...

Ngày tải lên: 11/04/2014, 10:32

... hydrogen-bonded to atoms at the protein surface in the first hydration shell, are located at well-ordered, fully occupied sites and can be mod- eled with confidence. Water molecules at longer dis- tances ... published macromolecular structures Alexander Wlodawer 1 , Wladek Minor 2,3 , Zbigniew Dauter 4 and Mariusz Jaskolski 5,6 1 Macromolecular Crystallography Laboratory, NCI, Frederick, MD...

Ngày tải lên: 16/03/2014, 06:20

Ngày tải lên: 06/08/2012, 14:52

Ngày tải lên: 06/08/2012, 14:53