macromolecular crystallography, part d
macromolecular crystallography, part d
... of
electron density corresponding to helices and sheets, and build a prelimin-
ary model. The theory and operation of both SOLVE and RESOLVE have
been described in detail.
1,4,5
In addition, an ... Crystallogr. D 56, 1413 (2000).
44
Z. Dauter, M. Dauter, and E. Dodson, Acta Crystallogr. D 58, 494 (2002).
45
Z. Dauter and M. Dauter, Structure 9, R21 (2001).
[1] how bijvoet made the differe...
Macromolecular Crystallography ppt
... Circular dichroism (CD) arises from the difference of absorption of right- and left-
handed polarized light. In protein solutions, far UV CD arises from amide bonds of
the peptides and depends on ... negatively charged SDS binds to the
denatured protein polypeptide in proportion to polypeptide size. The denatured pro-
teins with uniform negatively charged density are then separated in denat...
energetics of biological macromolecules, part d
... B)
Edited by David Barnes and David A. Sirbasku
Volume 148. Plant Cell Membranes
Edited by Lester Packer and Roland Douce
Volume 149. Drug and Enzyme Targeting (Part B)
Edited by Ralph Green and ... Methods
Edited by Ludwig Brand and Michael L. Johnson
Volume 211. DNA Structures (Part A: Synthesis and Physical Analysis of
DNA)
Edited by David M. J. Lilley and James E. Dahlberg
Volume 212. D...
numerical computer methods, part d
... David Barnes and David A. Sirbasku
Volume 147. Peptide Growth Factors (Part B)
Edited by David Barnes and David A. Sirbasku
Volume 148. Plant Cell Membranes
Edited by Lester Packer and Roland Douce
Volume ... XXXIV–LX
Edited by Edward A. Dennis and Martha G. Dennis
Volume 76. Hemoglobins
Edited by Eraldo Antonini,Luigi Rossi-Bernardi, and Emilia Chiancone
Volume 77. Detoxication and Drug Met...
recombinant dna part d
... and thus overcome the
drawbacks of the filamentous phage vectors described above. 2°,2°a
2t D. Denhardt, D. Dressier, and D. S. Ray (eds.), "The Single-Stranded DNA Phages."
Cold ... supplier.
Media, Nutritional Supplements, and Buffers
Use distilled water for media and buffers (dH20) and double-distilled
water (ddH20) for enzyme reactions.
All biochemicals can be purchase...
rna polymerase and associated factors, part d
... B)
Edited by David Barnes and David A. Sirbasku
Volume 148. Plant Cell Membranes
Edited by Lester Packer and Roland Douce
Volume 149. Drug and Enzyme Targeting (Part B)
Edited by Ralph Green and ... Enzymes
Edited by Gertrude E. Perlmann and Laszlo Lorand
Volume XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by Kivie Moldave and Lawrence Grossman
Volume XXI. Nucleic Acids (Part D...
small gtpases and their regulators, part d
... GDP/GDP. At time 0, 1 mM [3H]GDP was added to 20/xM FLAG-
RhoA/His6-RhoGDI in nucleotide exchange buffer and incubated at 22 °. Aliquots were taken
at the specified times, and MgCI2 was added ... and
a cleaved effector protein can be added to the beads at the same time.)
Wash the beads three to five times with binding buffer. Add 10 /zl 2×
SDS-PAGE sample buffer to the beads, and fractio...
Báo cáo khoa học: Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures potx
... hydrogen-bonded to atoms at the
protein surface in the first hydration shell, are located
at well-ordered, fully occupied sites and can be mod-
eled with confidence. Water molecules at longer dis-
tances ... published
macromolecular structures
Alexander Wlodawer
1
, Wladek Minor
2,3
, Zbigniew Dauter
4
and Mariusz Jaskolski
5,6
1 Macromolecular Crystallography Laboratory, NCI, Frederick, MD...
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