hemoglobin disorders, molecular methods and protocols
hemoglobin disorders, molecular methods and protocols
... Press
Hemoglobin
Disorders
Molecular Methods
and Protocols
Edited by
Ronald L. Nagel, MD
M E T H O D S I N M O L E C U L A R M E D I C I N E
TM
Hemoglobin
Disorders
Molecular Methods
and Protocols
Edited ... Acta 182, 301–308.
X-ray Crystallography of Hemoglobins 1
1
1
From:
Methods in Molecular Medicine, vol. 82: Hemoglobin Disorders: Molecular Methods and P...
Glycoprotein Methods and Protocols - P1
... Methods in Molecular Biology
TM
HUMANA PRESS
Glycoprotein
Methods
and Protocols
Edited by
Anthony P. Corfield
VOLUME 125
The Mucins
Methods in Molecular Biology
TM
HUMANA ... Mucins
Glycoprotein
Methods
and Protocols
Isolation of Large Gel-Forming Mucins 11
Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu-
cins were mixed with DNA...
Glycoprotein Methods and Protocols - P2
... 7.4, with 0.1% CaCl
2
at 37°C
Histological Methods for Detection of Mucin 29
29
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... nature and
limitations of the individual techniques, and (2) interpretation and reporting of mucin
staining.
1.1. Nature and Limitations of Mucin-Staining Methods
Mucin histo...
Glycoprotein Methods and Protocols - P3
... Detection and Quantitation of Mucins 45
45
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... in Glycoprotein
Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.
3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and
Protocols: The Mucins ... Nagma, K...
Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 57
57
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... poly-
L
-lysine for 5 min
and dried at room temperature.
3. Methods
3.1. Observation and Measurement
of Mucous Thickness on Unfixed Mucosal Sections (
see
Notes 1–4)
This is a rapid and simple metho...
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... filter paper.
19. Molecular weight marker: nonreduced monomeric and dimeric rat gastric mucin precur-
sors, molecular mass 300 and 600 kDa, respectively (8) (see Chapters 20 and 21).
20. Homogenization ... precu...
Glycoprotein Methods and Protocols - P6
... few separation techniques available to use with these
77
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... small gel, use 1.6 g of
agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of
buffer.
2. Leave to cool before pouring (hand hot) and insert well-fo...
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87
87
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin
populations with regard to mass and size utilizing rate zonal centrifugation and electron
microscopy. Other methods for the abso...
Glycoprotein Methods and Protocols - P8
... 113
113
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., Totowa, NJ
10
Amino Acid Analysis of Mucins
Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a
black residue. This residue appears to precipitate protein/amino acids, interferes with
114 Yan a...
Glycoprotein Methods and Protocols - P9
... Sequencing Glycopeptides 121
121
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... 3 and 8) and the length of sequence
obtained is dependent on the number and distribution of proline residues. Proline cleaves
with reduced efficiency in Edman sequencing (Fig. 1, vessel 1), and ... Kirk,...