hemoglobin disorders, molecular methods and protocols
hemoglobin disorders, molecular methods and protocols
... Press Hemoglobin Disorders Molecular Methods and Protocols Edited by Ronald L. Nagel, MD M E T H O D S I N M O L E C U L A R M E D I C I N E TM Hemoglobin Disorders Molecular Methods and Protocols Edited ... Acta 182, 301–308. X-ray Crystallography of Hemoglobins 1 1 1 From: Methods in Molecular Medicine, vol. 82: Hemoglobin Disorders: Molecular Methods and P...
Ngày tải lên: 11/04/2014, 09:45
Glycoprotein Methods and Protocols - P1
... Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P2
... 7.4, with 0.1% CaCl 2 at 37°C Histological Methods for Detection of Mucin 29 29 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histo...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P3
... Detection and Quantitation of Mucins 45 45 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple metho...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... filter paper. 19. Molecular weight marker: nonreduced monomeric and dimeric rat gastric mucin precur- sors, molecular mass 300 and 600 kDa, respectively (8) (see Chapters 20 and 21). 20. Homogenization ... precu...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P6
... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-fo...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the abso...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P8
... 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., Totowa, NJ 10 Amino Acid Analysis of Mucins Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a black residue. This residue appears to precipitate protein/amino acids, interferes with 114 Yan a...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P9
... Sequencing Glycopeptides 121 121 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... 3 and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues. Proline cleaves with reduced efficiency in Edman sequencing (Fig. 1, vessel 1), and ... Kirk,...
Ngày tải lên: 08/10/2012, 10:25