... Analysis of DNA Protein Interactions by Intrinsic Fluorescence Mark L. Carpenter, Anthony W. Oliver, and G. Geoff Kneale 491 34 Circular Dichroism for the Analysis of Protein DNA Interactions Mark ... Molecular Biology TM VOLUME 148 DNA Protein Interactions SECOND EDITION SECOND EDITION Principles and Protocols Principles and Protocols Edited by Tom Moss DNA Protein Interac...
Ngày tải lên: 08/04/2014, 12:51
dna protein interactions
... fully around the DNA. (*) Indicates the position of the radioactive label. + 3’ 5’ 00 _ complexed DNA - free DNA Fig. 2. Protein -DNA complexes are separated from free DNA by nondenaturing ... DNA- fragment. Lane 3: Labeled DNA- fragment, after Exo III treatment. Lane 4. Pattern of DNA- protein complex, obtained after Exo III treatment with both S- ends labeled. Lane 5...
Ngày tải lên: 11/04/2014, 09:40
... of a complex with plectonemic DNA. Engineering and design of protein- nucleic acid interactions – lessons from endonucleases Restriction endonucleases are the DNA protein bind- ers with the widest ... full-length protein can be extremely rewarding, as shown by the information obtained with the tumor suppressor protein BRCA2 and its interac- tion with DNA [44,45]. As compared wi...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx
... easily gain access to the RNA protein interface to increase the dissociation process. The promiscuity often reported for proteins M. Doetsch et al. Transient RNA protein interactions in RNA folding FEBS ... summarize the properties of the three protein classes, with the main focus being on RNA chaperones and annealer proteins. Specifically binding proteins A specific protein cofactor bi...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... of cob(II)alamin MS and incorporation of cobalamin into apo-MS is enhanced through specific protein protein interactions between the MSR FMN domain and MS. Abbreviations AD, activation domain; AqCbl, ... not observed when MSR was replaced by nNOSred or CPR, highlighting the need for specific protein protein interactions between MSR and hMS. Reactivation of hMS was found to be dependent...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... a protein. In Protein Structure (Creighton, T.E., ed.), 2nd edn, pp. 253–259. Oxford University Press, Oxford. 35. Privalov, P.L. (1979) Stability of proteins: small globular proteins. Adv. Protein ... (enzyme I of the PTS), the first protein in the cascade of proteins forming the PTS, to HPr, the histidine-phosphocarrier protein. HPr is the smallest protein in the protein cascade...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Gc recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions pot
... identification of protein interactions. In these techniques, protein protein Abbreviations EGFR, epidermal growth factor receptor; EGFP, enhanced green fluorescent protein; GFP, green fluorescent protein; ... detection of protein protein interactions In general, highly sensitive systems might detect even undesirable, feeble signals. To confirm the specificity of detection of protein pr...
Ngày tải lên: 05/03/2014, 23:20
Báo cáo khoa học: Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling doc
... – protein protein interactions Yeast–mammal chimeric Ga system Medici et al. [78] constructed an intelligent system for analysis of protein protein interactions by managing heterotrimeric G -protein ... signaling-based screening systems for protein protein interactions Small monomeric G -protein signaling in yeast Small monomeric G-proteins, such as Ras and Ras-like proteins, ar...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... for elucidating protein protein interactions and selecting specific binders to novel target proteins, and the first and second minireviews focus on the detection of protein protein interactions [8,9]. ... explorations of new genome sequences and protein structures have revealed various small, nonantibody scaffold proteins. Accurate structural descriptions of protein protein intera...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
... substrates. Protein protein interactions Multisite phosphorylation per se is not associated with switch-like responses [18,48]. We showed here that the characteristics of protein protein interactions, ... that determine the protein protein interactions: the concen- trations of the enzymes relative to the target protein, and the respective dissociation constants. Figure 5A shows th...
Ngày tải lên: 07/03/2014, 10:20