pcr mutation detection protocols

... Polyacrylamide Gel Electrophoresis 1 1 From: Methods in Molecular Biology, vol. 187: PCR Mutation Detection Protocols Edited by: B. D. M. Theophilus and R. Rapley © Humana Press Inc., Totowa, ... Wells Internal Labeling of DNA Probes 13 13 From: Methods in Molecular Biology, vol. 187: PCR Mutation Detection Protocols Edited by: B. D. M. Theophilus and R. Rapley © Humana Pres...

Ngày tải lên: 11/04/2014, 07:13

... authors. C. Gijavanekar et al. PCR detection of nearly any dengue virus strain FEBS Journal 278 (2011) 1676–1687 ª 2011 The Authors Journal compilation ª 2011 FEBS 1687 PCR detection of nearly any dengue ... RT -PCR for rapid detection and typing of dengue viruses. Virol J 5, 20. 13 Lai YL, Chung YK, Tan HC, Yap HF, Yap G, Ooi EE & Ng LC (2007) Cost-effective real-time reverse...

Ngày tải lên: 14/02/2014, 19:20

... Beijing, China xiv Contributors PCR: Basic Principles 1 I PERFORMING AND OPTIMIZING PCR PCR: Basic Principles 3 3 From: Methods in Molecular Biology, Vol. 192: PCR Cloning Protocols, 2nd Edition Edited ... during amplification has led to the development of kinetic PCR or quantitative PCR. Quantitative PCR (QPC) measures PCR: Basic Principles 15 PCR product accumulation during th...

Ngày tải lên: 08/04/2014, 12:52

... standards, etc.), and applications involving the PCR process (e.g., reverse-transcription PCR, nested PCR, multiplex PCR, nucleic acid sequencing, and detection of specic analytes). This collection ... in a PCR laboratory. 16 McDonagh Methods in Molecular Biology TM Methods in Molecular Biology TM PCR Protocols S ECOND E DITION Edited by John M. S. Bartlett David Stirling Volu...

Ngày tải lên: 08/04/2014, 12:52

... O’Connell RT -PCR Protocols VOLUME 193 Edited by Joe O’Connell RT -PCR Protocols RT -PCR in Biomedicine 5 Many quantitative RT -PCR approaches are based on competitive PCR (6). Essentially, a control PCR ... Winfield, KS xiv Contributors RT -PCR in Biomedicine 1 I INTRODUCTION 2 O'Connell RT -PCR in Biomedicine 3 3 From: Methods in Molecular Biology, vol. 193: RT -PCR Pr...

Ngày tải lên: 08/04/2014, 12:52

... by Vladimir V. Didenko In Situ Detection of DNA Damage Methods and Protocols VOLUME 203 Edited by Vladimir V. Didenko In Situ Detection of DNA Damage Methods and Protocols Labeling DNA Damage ... following sections are general protocols used in our laboratory for the detection of DNA fragmentation in situ using TdT. More detailed and application-specific protocols are given in...

Ngày tải lên: 11/04/2014, 09:47

... and Higuchi, R. (1989) Avoiding false positives with PCR Nature 339, 237,238. 8. Orrego, C (1990) Organizing a laboratory for PCR work. PCR Protocols. A Guide to Methods and Appltcations (Innis, ... phage 1 genome (see Notes 6,7) 2. AmpliWax PCR Gems (Perkm-Elmer). 3. Perkin-Elmer GeneAmp PCR Instrument System and PCR tubes with which the XL PCR reagents have been optimi...

Ngày tải lên: 11/04/2014, 10:01

... addition of short double-stranded DNA fragments (103). PCR Specificity and Performance 17 4.2. Nested PCR The use of nested PCR can often solve detection problems associated with clinical tissue ... examples in which the necessary sen- sitivity of detection was attained only thanks to nested PCR. This is mainly due to the fact that many PCR detection assays are not completely o...

Ngày tải lên: 11/04/2014, 10:01

... This cDNA may be used directly in PCR reactions or may be modified further (see Note 18). 3.2. Performing PCR 3.2.1. Basic PCR Protocol The ideal way to perform PCR is in a dedicated room, using ... 3.3.2.2. AC-IDE GEL ELECTROPHORESIS FOR DETECTION OF DIRECTLY LABELED PCR PRODUCTS For very sensitive detection and relative quantitation of PCR prod- ucts, the DNA fra...

Ngày tải lên: 11/04/2014, 10:01

... the PCR amplifications (1-4). Therefore, a number of protocols have been devised that address these technical issues, and allow effi- cient sequencing of either conventional double-stranded PCR ... 1-4). Many of these protocols are described in detail in this volume. An unrelated, but equally frustrating obstacle to the sequencing of PCR products arises when multiple PCR produc...

Ngày tải lên: 11/04/2014, 10:02