gene knockout protocols

... sequences. Gene Knockouts 1 1 From: Methods in Molecular Biology, vol. 158: Gene Knockout Protocols Edited by: M. J. Tymms and I. Kola © Humana Press Inc., Totowa, NJ Overview Gene Knockouts Paul ... usually a drug selection gene or a marker gene to analyze target gene expression. Insertion vectors (1,2), which may offer increased gene- targeting frequencies at a given genet...

Ngày tải lên: 11/04/2014, 00:38

... Huang, L. (1995) Cationic liposome-mediated gene transfer. Gene Ther. 2, 710–722. 13. Gao, X. (1997) Cationic lipid-based gene delivery: an update, in Gene Therapy for Diseases of the Lung (Brigham, ... Biology Methods in Molecular Biology TM TM VOLUME 133 HUMANA PRESS HUMANA PRESS Gene Targeting Protocols Gene Targeting Protocols Edited by Eric B. Kmiec Cationic Lipid Nucleic...

Ngày tải lên: 10/04/2014, 22:26

... Biology TM Edited by Peter E. Vaillancourt E. coli Gene Expression Protocols VOLUME 205 Edited by Peter E. Vaillancourt E. coli Gene Expression Protocols Cold-Inducible Promoters 7 4. LB medium: ... R. LAVALLIE • Genetics Institute/Wyeth Research, Cambridge, MA H ANS LEHRACH • Max-Planck-Institute for Molecular Genetics, Berlin, Germany; PROT@GEN, Bochum, Germany Z HIJIAN LU • Genetic...

Ngày tải lên: 10/04/2014, 22:36

... Press M E T H O D S I N M O L E C U L A R M E D I C I N E TM Gene Therapy Protocols Edited by Jeffrey R. Morgan Second Edition Gene Therapy Protocols Second Edition Edited by Jeffrey R. Morgan 20 ... DNA. Gene Ther. 6, 1258–1266. 21. Zhang, G., Budker, V., and Wolff, J. A. (1999) High levels of foreign gene ex- pression in hepatocytes after tail vein injections of naked plasmid DN...

Ngày tải lên: 10/04/2014, 22:53

... resulted in levels of reporter gene expression detectable above background levels (13). This com- pares favorably to gene transfer mediated by other DNA-mediated gene trans- fer methods, where ... levels of gene transfer achieved by free viral facilitation of molecular conjugate-mediated gene transfer, adenovirus m the linked configuration is capable of significantly higher lev...

Ngày tải lên: 11/04/2014, 09:42

... Biology TM Edited by Maurizio Federico Lentivirus Gene Engineering Protocols VOLUME 229 Edited by Maurizio Federico Lentivirus Gene Engineering Protocols 22 Lotti and Mavilio be practically verifi ... therapeutic agent. Gene Ther. 7, 20–23. 3. Vigna, E. and Naldini, L. (2000) Lentiviral vectors: excellent tools for experimen- tal gene transfer and promising candidates for gene t...

Ngày tải lên: 11/04/2014, 09:49

... artifact: Generation of heteroduplexes. Am. J. Hum. Genet. 45, 337–339. Mapping Human Genes 25 25 From: Methods in Molecular Medicine, vol. 30: Vascular Disease: Molecular Biology and Gene Therapy Protocols Edited ... Disease: Molecular Biology and Gene Therapy Protocols Edited by: A. H. Baker © Humana Press Inc., Totowa, NJ 2 Analysis of Genetic Variants in Cardiovascular Risk Genes...

Ngày tải lên: 11/04/2014, 10:39

... internal standards for reference genes and target genes and gene specific primers from Gene Express, Inc. as described earlier. As with the reference gene, the target gene NT/CT must be greater than ... many genes simultaneously. An internal standard CT is prepared for each gene, cloned to generate enough for >10 9 assays and CTs for up to 1000 genes are mixed together. Each targe...

Ngày tải lên: 10/04/2014, 22:32

... Biology TM Gene Probes Edited by Marilena Aquino de Muro Ralph Rapley VOLUME 179 Principles and Protocols Gene Probes Edited by Marilena Aquino de Muro Ralph Rapley Principles and Protocols 20 ... finger- printing and hybridization in situ. Hum. Genet. 82, 227–233. Photobiotin Labeling 19 19 From: Methods in Molecular Biology, vol. 179: Gene Probes: Principles and Protocols Edited...

Ngày tải lên: 10/04/2014, 22:46