genetic recombination, reviews and protocols

... kit) and a DNA digestion with HindIII plus BamHI, cell lines containing pLB4 should display a 3.9-kb and a 2.5-kb band and cell lines containing pBR3 should display a 3.9-kb and a 1.4-kb band ... discussion and further Edited by Alan S. Waldman Genetic Recombination Reviews and Protocols Volume 262 METHODS IN MOLECULAR BIOLOGY TM METHODS IN MOLECULAR BIOLOGY TM Edited by Ala...

Ngày tải lên: 10/04/2014, 23:06

... small increases (~20%) were observed for NR1 and NR2B, but these were not signifi cant, and Humana Press Drugs of Abuse Neurological Reviews and Protocols Edited by John Q. Wang Humana Press M ... between DA and gluta- mate is complex and remains controversial (40). Repeated psychostimulant administration leads to profound changes in both DA and glutamate trans- Psychostimul...

Ngày tải lên: 11/04/2014, 01:57

... the integration between λ DNA and E. coli DNA. §1.5 Site-specific Recombination Chapter 14 Genetic Recombination and Genetic Engineering The biochemistry and molecular biology department ... Salmonella typhimurium flagella Hin rH1 rH1 Recombination activating gene enzyme (RAG1 and RAG2) CACAGTG (12/23) ACAAAAACC GTGTCAC TGTTTTTGG RSS Recombination signal sequence...

Ngày tải lên: 13/03/2014, 19:25

... PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA PRESS Edited by Anthony P. Corfield The Mucins Glycoprotein Methods and Protocols Isolation ... acid func- tions and overall sequence similarity to prepro- von Willebrand factor. J. Biol. Chem. 269, 2440–2446. 5. Moniaux, N., Nollet, S., Porchet, N., De...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties, Func...

Ngày tải lên: 08/10/2012, 10:25

... Methods and Protocols: The Mucins (Corfield, T. ed.), Humana, Totowa, NJ. 4. Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their ... sample volume, and superior wash- ing). For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samp...

Ngày tải lên: 08/10/2012, 10:25

... will be greatly reduced and discontinuous. 7. Careful handling of the cryostat sections on the slide during fixing and staining is impor- tant because excessive washing and so on can remove mucus ... to rat, frog and human gastric mucosa: influence of feeding prostaglandin, N-acetyl-cysteine and other agents. Clin. Sci. 63, 187–195. 4. Bell, A. E., Allen, A., Morris, E. R., and Ro...

Ngày tải lên: 08/10/2012, 10:25

... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager. 4. The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several ref...

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... Separation and Identification of Mucin 77 7 Separation and Identification of Mucins and Their Glycoforms David J. Thornton, Nagma Khan, and John K. Sheehan 1. Introduction This ......

Ngày tải lên: 08/10/2012, 10:25

... K. E., and Chapman J. A. (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing. J. Ultrastruct. Res. 91, 66–76. 14. Kawahara K and Tanford C. (1966) Viscosity and density ... MUC5B, and MUC6 (1–4)—are gel-forming mucins, and it is possible that there are others. In isolating a mixture of mucins from mucus, there is consider- able scope for heterogeneity i...

Ngày tải lên: 08/10/2012, 10:25