e coli gene expression protocols
e. coli gene expression protocols
... Biology TM HUMANA PRESS Methods in Molecular Biology TM Edited by Peter E. Vaillancourt E. coli Gene Expression Protocols VOLUME 205 Edited by Peter E. Vaillancourt E. coli Gene Expression Protocols Cold-Inducible ... temperature downshifts between 37, 29, 21, and 13°C. However, re-induction is inefficient in tempera- ture cycling experiments and requires that the cells be...
Ngày tải lên: 10/04/2014, 22:36
Luận văn nghiên cứu sự phân bố của e coli gây bệnh tiêu chảy theo kiểu gene ở lợn con sau cai sữa tại một số tỉnh phía bắc
... E. coli gây bệnh được chia thành 6 loại là: Enterotoxigenic E. coli (ETEC), Enteroinvasive E. coli (EIEC), Enterohemorrhagic E. coli (EHEC), Enteropathogenic E. coli (EPEC), Enteroaggregative E. coli ... hợp đó. Enteroaggregative E. coli - EAggEc hay EAEC Yếu tố ñộc lực: EAEC là nhóm E. coli bám dính vào các tế bào HEp-2 theo kiểu Aggregative adherence (AA). Nhóm...
Ngày tải lên: 28/11/2013, 23:59
Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... the respective sequences. These cell lines were kindly provided by F Lehembre and G Christofori (Center of Biomedicine, University of Basel, Switzerland) and details will be pub- lished elsewhere. Plasmids ... To determine whether the disruption of cell–cell adhesion by Decma influenced expression of the uPA gene, we examined change in uPA mRNA levels. Northern blot analysis showed only bar...
Ngày tải lên: 19/02/2014, 05:20
Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
... Having determined the o verall elasticity of DNA gyrase gene- expression to supercoiling, we became interested in quan- tifying the extent to which topoisomerase I gene expression normally depends ... that at very low activities of topoisomerase I the DNA supercoiling d epended even more weakly, if at all, on the enzyme. At wild-type expression levels, the dependence appeared to be s...
Ngày tải lên: 18/03/2014, 01:20
E. coli Shiga Toxin Methods and Protocols doc
... to cloned stx 1 and stx 2 genes and their respective nucleotide sequences enabled the development of DNA and oligonucleotide probes for the detection of STEC (reviewed in ref. 5). The introduction ... for stx 2 -related genes, for which significant variation has been reported (reviewed in ref. 5). Sequence divergence between the primer and its target (particularly at the 3' end of the...
Ngày tải lên: 23/03/2014, 07:20
gene expression profiling, methods and protocols
... loaded into the reaction. Each target gene and reference gene is measured relative to its respective inter- nal standard in the SMIS. Because each target gene and reference gene is simul- taneously ... the samples being evaluated. 2.1.3. Choice of Reference Gene Many different genes are used as reference genes. No single gene is ideal for all studies. For example, β-actin varies l...
Ngày tải lên: 10/04/2014, 22:32
e. coli, shiga toxin methods and protocols
... stimulation (2,17). The immune response to STEC protein antigens encoded by genes cluster- ing on the locus of enterocyte attachment and effacement (LEE) has recently been evaluated independently by two ... methods need to be very sensitive and require minimal specimen volumes. Shiga toxigenic E. coli diagnostic methods are based on the detection of the presence of either Stx or stx genes...
Ngày tải lên: 10/04/2014, 23:03
gene transfer and expression protocols
... can reveal both positive and negative regulatory elements that define within a cell type the level of expression of a given gene. Most cellular genes require enhancer elements for efficient ... use the regimen that gives better survival. 4. Transfection vectors also greatly influence transformation frequencies. Use of a vector that expresses the marker gene weakly, or use of a marker...
Ngày tải lên: 11/04/2014, 09:42