diabetes mellitus, methods and protocols
diabetes mellitus, methods and protocols
... Casteele, and Heimberg
Humana Press
Diabetes
Mellitus
Methods and Protocols
Edited by
Sabire Özcan
Humana Press
M E T H O D S I N M O L E C U L A R M E D I C I N E
TM
Diabetes
Mellitus
Methods and Protocols
Edited ... designated insulin I and II, are
present at a ratio of 1:3 in the mouse and 4: 1 in the rat (insulin I:II) (6).
From: Methods in Molecular Medicine, vol....
Glycoprotein Methods and Protocols - P1
... Methods in Molecular Biology
TM
HUMANA PRESS
Glycoprotein
Methods
and Protocols
Edited by
Anthony P. Corfield
VOLUME 125
The Mucins
Methods in Molecular Biology
TM
HUMANA ... Mucins
Glycoprotein
Methods
and Protocols
Isolation of Large Gel-Forming Mucins 11
Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu-
cins were mixed with DNA and...
Glycoprotein Methods and Protocols - P2
... nature and
limitations of the individual techniques, and (2) interpretation and reporting of mucin
staining.
1.1. Nature and Limitations of Mucin-Staining Methods
Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa,
NJ.
50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...
Glycoprotein Methods and Protocols - P3
... in Glycoprotein
Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.
3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and
Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi-
cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The
M...
Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 57
57
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... poly-
L
-lysine for 5 min
and dried at room temperature.
3. Methods
3.1. Observation and Measurement
of Mucous Thickness on Unfixed Mucosal Sections (
see
Notes 1–4)
This is a rapid and simple method...
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... NJ
6
Quantitation of Biosynthesis
and Secretion of Mucin Using Metabolic Labeling
Jan Dekker, B. Jan-Willem Van Klinken,
Hans A. Büller, and Alexandra W. C. Einerhand
1. Introduction
Most epithelial ... PhosphorImager....
Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of
agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of
buffer.
2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these
77
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Human...
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87
87
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin
populations with regard to mass and size utilizing rate zonal centrifugation and electron
microscopy. Other methods for the absolu...
Glycoprotein Methods and Protocols - P8
... 113
113
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., Totowa, NJ
10
Amino Acid Analysis of Mucins
Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a
black residue. This residue appears to precipitate protein/amino acids, interferes with
114 Yan and...
Glycoprotein Methods and Protocols - P9
... Sequencing Glycopeptides 121
121
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... 3 and 8) and the length of sequence
obtained is dependent on the number and distribution of proline residues. Proline cleaves
with reduced efficiency in Edman sequencing (Fig. 1, vessel 1), and ... Kirk, M....
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