adhesion protein protocols

... antibody was omitted. 3.4.6. Immunohistochemistry of Adhesion Proteins 1. Immunohistochemistry is a valuable technique for detection of adhesion proteins in tissue sections. In fact, most selectins, ... 96 TM Humana Press Edited by Elisabetta Dejana and Monica Corada ADHESION PROTEIN PROTOCOLS 20 DeLisser CD31 and the role of CD31 adhesion in the formation of intraendothelial cell...

Ngày tải lên: 10/04/2014, 22:13

... Biology TM Edited by Barry S. Selinsky Membrane Protein Protocols VOLUME 228 Expression, Purification, and Characterization Edited by Barry S. Selinsky Membrane Protein Protocols Expression, Purification, and ... Elute proteins bound to Ni 2+ -Sepharose with elution buffer. Proteins thus eluted are analyzed by SDS-PAGE, Western blotting, and activity tests (see Subheading 3.6.). The p...

Ngày tải lên: 08/04/2014, 12:51

... Molecular Biology TM Calcium-Binding Protein Protocols Edited by Hans J. Vogel VOLUME 173 Volume II Methods and Techniques Edited by Hans J. Vogel Calcium-Binding Protein Protocols Volume II Methods and ... Multifunctional S100 Protein Family Claus W. Heizmann 5Ca 2+ Binding to Proteins Containing γ-Carboxyglutamic Acid Residues Egon Persson 6The Caseins of Milk as Calcium-Binding P...

Ngày tải lên: 11/04/2014, 00:23

... I Reviews and Case Studies Calcium-Binding Protein Protocols Calcium-Binding Proteins 3 3 From: Methods in Molecular Biology , vol. 172: Calcium-Binding Protein Protocols, Vol. 1: Reviews and Case Studies Edited ... Ca 2+ -binding proteins, the protein kinases C (27). Protein kinases C bind Ca 2+ through their C2 domain calcium-binding motifs; it is now known that C2 domains exist...

Ngày tải lên: 11/04/2014, 00:24

... Biology TM Edited by Barry S. Selinsky Membrane Protein Protocols VOLUME 228 Expression, Purification, and Characterization Edited by Barry S. Selinsky Membrane Protein Protocols Expression, Purification, and ... par- ticularly for small, cytoplasmic proteins. Heterologously expressed membrane proteins are often toxic to E. coli, which prevents cell growth and limits protein yields....

Ngày tải lên: 11/04/2014, 09:50

... Interacting Proteins Interacting pairs of proteins can be identified in vwo using a clever system developed by Stanley Fields and coworkers (38,39). Known as the two-hybrid system, the interacting proteins ... Transposon 9 of E coli (53). 4.1.2. Protein Characterrstics CAT is a trimertc protein comprtsing three ldentrcal subunits of 25,000 Dalton (54). The CAT protein is relattv...

Ngày tải lên: 11/04/2014, 10:28

... Protein Phosphorylation John Colyer 603 91 Mass Spectrometric Analysis of Protein Phosphorylation Débora BoneNfant, Thierry Mini, and Paul Jenö 609 92 Identification of Proteins Modified by Protein ( D-Aspartyl/L-Isoaspartyl) ... Research, Mayo Clinic, Rochester, MN Contributors xxi Protein Protocols Edited by John M. Walker The Handbook SECOND EDITION Humana Press Protein Protoc...

Ngày tải lên: 11/04/2014, 10:36

... E-rosetting assay and heterotypic cell adhesion assay indicated that cVY exhibits cell -adhesion inhibition better than cER. The cER peptide may potentially bind to the CD58 protein as indicated by the docking ... adhesion complex between human CD2 and CD58 (LFA-3) counter-receptors. Cell 97 , 791–803. 31. Rose, G.D., Gierasch, L.M. & Sm ith, J .A. (1985) Turns in pep- tides and pr...

Ngày tải lên: 19/02/2014, 13:20

... and cell adhesion protein in Artemia (Eur. J. Biochem. 271) 4025 Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins Alden ... embryos (33 lg protein) ; NEE, ovisacs containing nonencysted embryos (34 lg protein) ; GF, gravid females somatic tissue (126 lg protein) ; NGF, nongravid adult females (135 lg...

Ngày tải lên: 07/03/2014, 16:20

... increased NKL1 lymphocyte adhesion and more weakly CEMT4 lymphocyte adhesion. On the other hand, no change in NKL2 adhesion was observed. Simins et al. [17] showed that ARM-1 promoted cell adhesion when overexpressed ... to specific tissues. Adhesion- regulating molecule-1 (ARM-1) is an adhesion- regulating molecule previously identified on T cells. It does not belong to any known fami...

Ngày tải lên: 07/03/2014, 17:20