antibody engineering, methods and protocols

... efficient. 20 Johnson and Wu Methods in Molecular Biology TM Methods in Molecular Biology TM Edited by Benny K. C. Lo Antibody Engineering VOLUME 248 Methods and Protocols Methods and Protocols Antibody Engineering Edited ... between positions 27 and 28 (27A, 27B, 27C, 27D, 27E, and 27F) and between 97 and 98 (97A and 97B), which was later changed to between 95...

Ngày tải lên: 10/04/2014, 11:01

... UV monitor and collect fractions using a fraction col- lector and subject to the appropriate carbohydrate and antibody analyses. 3.6. Analysis of Mucins Methods for the detection and analysis ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins w...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...

Ngày tải lên: 08/10/2012, 10:25

... Inc., Totowa, NJ 4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods Michael A. McGuckin and David J. Thornton 1. Introduction Detection and quantitation of mucins ... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and...

Ngày tải lên: 08/10/2012, 10:25

... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager....

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Human...

Ngày tải lên: 08/10/2012, 10:25

... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolu...

Ngày tải lên: 08/10/2012, 10:25

... 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., Totowa, NJ 10 Amino Acid Analysis of Mucins Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a black residue. This residue appears to precipitate protein/amino acids, interferes with 114 Yan and...

Ngày tải lên: 08/10/2012, 10:25