antibody engineering, methods and protocols
antibody engineering, methods and protocols
... efficient.
20 Johnson and Wu
Methods in Molecular Biology
TM
Methods in Molecular Biology
TM
Edited by
Benny K. C. Lo
Antibody
Engineering
VOLUME 248
Methods and Protocols
Methods and Protocols
Antibody
Engineering
Edited ... between
positions 27 and 28 (27A, 27B, 27C, 27D, 27E, and 27F) and between 97 and
98 (97A and 97B), which was later changed to between 95...
Glycoprotein Methods and Protocols - P1
... UV monitor and collect fractions using a fraction col-
lector and subject to the appropriate carbohydrate and antibody analyses.
3.6. Analysis of Mucins
Methods for the detection and analysis ... Mucins
Glycoprotein
Methods
and Protocols
Isolation of Large Gel-Forming Mucins 11
Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu-
cins w...
Glycoprotein Methods and Protocols - P2
... nature and
limitations of the individual techniques, and (2) interpretation and reporting of mucin
staining.
1.1. Nature and Limitations of Mucin-Staining Methods
Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa,
NJ.
50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...
Glycoprotein Methods and Protocols - P3
... Inc., Totowa, NJ
4
Detection and Quantitation of Mucins
Using Chemical, Lectin, and Antibody Methods
Michael A. McGuckin and David J. Thornton
1. Introduction
Detection and quantitation of mucins ... in Glycoprotein
Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.
3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and...
Glycoprotein Methods and Protocols - P4
... GI Adherent Mucous Gel Barrier 57
57
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., ... poly-
L
-lysine for 5 min
and dried at room temperature.
3. Methods
3.1. Observation and Measurement
of Mucous Thickness on Unfixed Mucosal Sections (
see
Notes 1–4)
This is a rapid and simple method...
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... NJ
6
Quantitation of Biosynthesis
and Secretion of Mucin Using Metabolic Labeling
Jan Dekker, B. Jan-Willem Van Klinken,
Hans A. Büller, and Alexandra W. C. Einerhand
1. Introduction
Most epithelial ... PhosphorImager....
Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of
agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of
buffer.
2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these
77
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Human...
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 87
87
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin
populations with regard to mass and size utilizing rate zonal centrifugation and electron
microscopy. Other methods for the absolu...
Glycoprotein Methods and Protocols - P8
... 113
113
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press Inc., Totowa, NJ
10
Amino Acid Analysis of Mucins
Jun X. Yan and ... caramelized and further charcoaled, and the acid hydrolysis results in a
black residue. This residue appears to precipitate protein/amino acids, interferes with
114 Yan and...