... Theodosiou, and Xu
Table 5
Strains for Clones in Developing and Internal Tissues
Chromosomes Strains Footnotes
X w P[mini -w+ hsπM]5A, 10D FRT18A; hsFLP3, MKRS/TM6B
a
w P[mini -w+ hsNM]8A FRT18A
a
w ... Theodosiou, and Xu
developmental profile of the tissue (s) you wish to study (see Fig. 3). For Ey-FLP or GAL4/
UAS-FLP, FLP is expressed and will get large clones.
6. When heat...
... formation.
Developmental
Biology
Protocols
Volume II
Edited by
Rocky S. Tuan
Cecilia W. Lo
Developmental
Biology
Protocols
Volume II
Edited by
Rocky S. Tuan
Cecilia W. Lo
HUMANA PRESS
HUMANA PRESS
Methods in Molecular ... laboratories and our colleagues for useful discus-
sions (e.g., Prof. Anton Neff) and editing (Susan Duhon) of this manuscript. Most of
t...
... Theodosiou, and Xu
Table 5
Strains for Clones in Developing and Internal Tissues
Chromosomes Strains Footnotes
X w P[mini -w+ hsπM]5A, 10D FRT18A; hsFLP3, MKRS/TM6B
a
w P[mini -w+ hsNM]8A FRT18A
a
w ... Theodosiou, and Xu
developmental profile of the tissue (s) you wish to study (see Fig. 3). For Ey-FLP or GAL4/
UAS-FLP, FLP is expressed and will get large clones.
6. When heat...
... to
metamorphosis in less than 30 d. High densities and lower algal densities will slow down
the rate of development. P. ochraceus develops more slowly and can take up to 8 wk until
metamorphosis. However, ... the sea urchins and screen support are removed, and as much seawater as pos-
sible (without losing eggs) is carefully decanted to waste. The settled eggs are then resus-
pended b...
... DAB, and OsO
4
(Fig. 2A). In the RCS
Fig. 1. Immunoblot analysis of GSH-PO molecules in retinas of Wistar rats (W)
and RCS rats (R). (Open arrows: GSH-PO of cytosol, closed arrows: GSH-PO of ... Specimens Reacted with
HRP-Conjugated Anti-GSH-PO, DAB, and OsO
4
(Methylene-Green Staining)
Photoreceptor outer segments of Wistar rats were well-developed, and
negative-stained with anti...
... facilitate
and encourage developmental biology and embryology laboratory experiences that bring students to-
gether with fascinating and dynamic developing systems. Professional biologists and nonbiologists ... separated, allow the cells to settle and pour off the supernatant.
Resuspend in normal seawater and proceed to the Analysis of Results section that follows.
Analysis of...
... road stretch files of wagons,
while people with wallets on their backs are trudging along and shadows flit backwards
and forwards; on both sides she can see forests through the cold harsh mist. ... broom, and swept every corner five times over. She lingered
for a long time in the room where mamma was sitting. She was evidently oppressed by
her isolation, and she was longing to expr...