developmental biology protocols - rocky s tuan and cecilia w lo

... Theodosiou, and Xu Table 5 Strains for Clones in Developing and Internal Tissues Chromosomes Strains Footnotes X w P[mini -w+ hsπM]5A, 10D FRT18A; hsFLP3, MKRS/TM6B a w P[mini -w+ hsNM]8A FRT18A a w ... Theodosiou, and Xu developmental profile of the tissue (s) you wish to study (see Fig. 3). For Ey-FLP or GAL4/ UAS-FLP, FLP is expressed and will get large clones. 6. When heat...

Ngày tải lên: 08/04/2014, 12:56

... formation. Developmental Biology Protocols Volume II Edited by Rocky S. Tuan Cecilia W. Lo Developmental Biology Protocols Volume II Edited by Rocky S. Tuan Cecilia W. Lo HUMANA PRESS HUMANA PRESS Methods in Molecular ... laboratories and our colleagues for useful discus- sions (e.g., Prof. Anton Neff) and editing (Susan Duhon) of this manuscript. Most of t...

Ngày tải lên: 10/04/2014, 22:59

... Theodosiou, and Xu Table 5 Strains for Clones in Developing and Internal Tissues Chromosomes Strains Footnotes X w P[mini -w+ hsπM]5A, 10D FRT18A; hsFLP3, MKRS/TM6B a w P[mini -w+ hsNM]8A FRT18A a w ... Theodosiou, and Xu developmental profile of the tissue (s) you wish to study (see Fig. 3). For Ey-FLP or GAL4/ UAS-FLP, FLP is expressed and will get large clones. 6. When heat...

Ngày tải lên: 10/04/2014, 23:01

... to metamorphosis in less than 30 d. High densities and lower algal densities will slow down the rate of development. P. ochraceus develops more slowly and can take up to 8 wk until metamorphosis. However, ... the sea urchins and screen support are removed, and as much seawater as pos- sible (without losing eggs) is carefully decanted to waste. The settled eggs are then resus- pended b...

Ngày tải lên: 11/04/2014, 02:14

... DAB, and OsO 4 (Fig. 2A). In the RCS Fig. 1. Immunoblot analysis of GSH-PO molecules in retinas of Wistar rats (W) and RCS rats (R). (Open arrows: GSH-PO of cytosol, closed arrows: GSH-PO of ... Specimens Reacted with HRP-Conjugated Anti-GSH-PO, DAB, and OsO 4 (Methylene-Green Staining) Photoreceptor outer segments of Wistar rats were well-developed, and negative-stained with anti...

Ngày tải lên: 11/04/2014, 10:00

... facilitate and encourage developmental biology and embryology laboratory experiences that bring students to- gether with fascinating and dynamic developing systems. Professional biologists and nonbiologists ... separated, allow the cells to settle and pour off the supernatant. Resuspend in normal seawater and proceed to the Analysis of Results section that follows. Analysis of...

Ngày tải lên: 11/04/2014, 10:01

... road stretch files of wagons, while people with wallets on their backs are trudging along and shadows flit backwards and forwards; on both sides she can see forests through the cold harsh mist. ... broom, and swept every corner five times over. She lingered for a long time in the room where mamma was sitting. She was evidently oppressed by her isolation, and she was longing to expr...

Ngày tải lên: 06/11/2012, 14:13

Ngày tải lên: 25/01/2013, 16:44