flow cytometry protocols 2d ed - teresa s hawley

... collected by this lens is defined by the diameter of the lens and its distance from the analy- sis point and is called side scatter light (“ssc”) or 90° light scatter. Laser light is scattered to ... color as the laser beam striking the cell. In a single laser system, this is usually 488-nm light from an argon ion laser; in a system with two or more lasers, the scattered light is also usually...

Ngày tải lên: 08/04/2014, 12:51

... post-PCR area. 2.3. Consumables Disposable plastics rather than reusable glass should be used wherever possible, and high-quality consumables, for example, Rnase-free plasticware, should be used ... pellets from dispersed tissues or cell cultures (omitting the red blood lysis step. 2. Materials This method uses standard chemicals that can be obtained from any major supplier; we use Sigma....

Ngày tải lên: 08/04/2014, 12:52

... fluorochrome-labeled antibody. This sample is almost always acquired as the first sample in a set because tt serves as a baseline reference point. FSC and SSC are usually adjusted so that the cells of ... fixatiotipermeabrlization solution success- fully used for TdT staining in flow cytometry was Beckton-Dickmson s (Mountainview, CA) diethylene glycol-based FACS red cell-lysing solu...

Ngày tải lên: 11/04/2014, 09:41

... Constants 277 Rate Constants and Mechanism 283 Appendix 284 Glossary 287 Index 319 Since the first edition of this series, we have witnessed the birth of “mol- ecular medicine,” using biochemistry, ... stabilize the formation of the helix or sheet struc- ture. The stability of secondary structure is also influenced by surround- ing structures (Fig. 2-3 ). Secondary structure may be stabiliz...

Ngày tải lên: 08/04/2014, 12:40

... DNA single-strand breaks but cells swollen were ob- served.[48] This results from a loss of plasma mem- brane integrity which suggests nuclear chromatin decondenzation considered as necrosis biomarker.[55, ... 10.000 cells were counted and the re- sults were presented as histograms in logarith- mic-modus. The specific fluorescence intensity was calculated as the ratio of the geometric mean...

Ngày tải lên: 26/10/2012, 09:48

... wondered D. studied 14.A. sacrificed B. finished C. fixed D. seized 15. A. needed B. booked C. stopped D. washed 16. A. loved B. teased C. washed D. rained 17. A. packed B. punched C. pleased D. ... pushed 18. A. filled B. naked C. suited D. wicked 19. A. caused B. increased C. practised D. promised 20. A. washed B. parted C. passed D. barked 21. A. killed B. cured C. crashed D. waived 22....

Ngày tải lên: 16/07/2013, 01:25

... co-expressed IL-lβ/CCR5, IL-2/CCR5, and IFN-γ/CCR5, while 3% co-expressed IL-4/CCR4, and 1% co-expressed IL-10/CCR4. After stimulating CD4 + T cells with MLIF, 15% cells co-expressed IL-1β/CCR5, ... events was conducted in flow cytometry FACScan (BD Biosciences, Sa Jose, USA). For analysis, Facs Diva and Win MDI 2.8 software were used. The results showed that CD4 + T cells control...

Ngày tải lên: 08/03/2014, 19:20

... almost universal con- sensus amongst cosmologists as to the best description of our Universe. Nevertheless, it is a model with a major surprise — the belief that our Universe is ... waves, ripples in space-time itself, and ultimately of using them to observe astronomical events such as colliding stars. The advent of large ground-based and satellite-based telesc...

Ngày tải lên: 17/03/2014, 13:34

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Ngày tải lên: 17/03/2014, 13:36