chiral separations methods and protocols - gerald gubitz

... Biology TM Edited by Gerald Gübitz Martin G. Schmid Chiral Separations Methods and Protocols VOLUME 243 Edited by Gerald Gübitz Martin G. Schmid Chiral Separations Methods and Protocols 8 Gübitz and Schmid carbamate ... sulfated CDs, sulfobutyl- and sulfoethyl-β-CD, carboxymethyl-β-CD, and succinyl-β-CD, were applied to the chiral separation of neutral and ba...

Ngày tải lên: 08/04/2014, 12:50

... Biology TM Methods in Molecular Biology TM Edited by Gerald Gübitz Martin G. Schmid Chiral Separations Methods and Protocols VOLUME 243 Edited by Gerald Gübitz Martin G. Schmid Chiral Separations Methods ... (R )-4 -hydroxyphenylglycine. Chirality 10, 592–599. 49. Lin, C E. and Lin, C H. (1994) Enantiomer separation of amino-acids on a chiral stationary-phase deriv...

Ngày tải lên: 10/04/2014, 23:02

... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and subjected ... different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based meth- ods such as slot-blotting and stainin...

Ngày tải lên: 08/10/2012, 10:25

... mounting, and its relative stability. There are, however, several other alternatives, such as 3-amino- 9-ethylcarbazole (AEC), for use with horseradish peroxidase (HRP); 5-bromo-4-chloro- 3-indolyl ... side chain substituted O-acetylated sialic acids: the PAT- KOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedures. Histochem. J. 16, 623–639. 8. Veh, R. W., Meesen, D., Kuntz, D., and May, B. (198...

Ngày tải lên: 08/10/2012, 10:25

... of competitive inhibitors of O-glycosylation, such as benzyl 2-acetamido- 2-deoxy- - D -galactopyranoside, without adversely affecting cells (9). With the eluci- dation of more sequence data ... carbohydrate or core pro- tein specificity), and lower sensitivity than slot-blot and immunoassay methods. Chap- Detection and Quantitation of Mucins 47 ter 1 (2) discusses these assays and...

Ngày tải lên: 08/10/2012, 10:25

... mL of glycerol. 8. Poly- L -lysine-coated slides: slides are immersed in 0.01% (w/v) poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous ... rat, frog and human gastric mucosa: influence of feeding prostaglandin, N-acetyl-cysteine and other agents. Clin. Sci. 63, 187–195. 4. Bell, A. E., Allen, A., Morris, E. R., and Ross-...

Ngày tải lên: 08/10/2012, 10:25

... interest in the homogenate and medium by Western-type dot-blot procedure. Spot aliquots of the homogenates or media on nitrocel- lulose paper, using the dot-blot apparatus and air-dry for 5 min. Perform ... [ 35 S]sulfate-labeled, TCA-precipitable proteins in tis- sue homogenate (j1) and medium (j2) (cpm/mL) (Note 10). k. Mature [ 35 S]sulfate-labeled mucin band in tissue homogenate (k1...

Ngày tải lên: 08/10/2012, 10:25

... prepared from the gel-forming mucins isolated from other epithelial secretions (respiratory and cer- vical) and from intestinal cell lines (HT-29 and PC/AA). In this example the electro- phoretic mobility ... monitored with a periodic acid-Schiff (PAS) assay and with a variety of lectins and mucin- or carbohydrate-specific antibodies (for a discussion of the relative merits and dr...

Ngày tải lên: 08/10/2012, 10:25

... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... Swing-out rotors and tubes (see Note 1). 5. Gradient maker (see Note 2). 8 96 Sheehan and Thornton 12. Koller, T., Harford, A. G., Lee, Y. K., and Beer, M. (1969) New methods for the prepara- tion ... M...

Ngày tải lên: 08/10/2012, 10:25