cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks

... BIOLOGY TM Edited by Tim Humphrey Gavin Brooks Cell Cycle Control Volume 296 Mechanisms and Protocols Edited by Tim Humphrey Gavin Brooks Cell Cycle Control Mechanisms and Protocols The Budding and Fission ... MOLECULAR BIOLOGY TM Edited by Tim Humphrey Gavin Brooks Cell Cycle Control Volume 296 Mechanisms and Protocols Edited by...

... by incubating with 10 mL PBS containing 10 mM EDTA and 4 mg/mL Lidocaine-HCL for 10 min, followed by vigorous pipetting. 6. Collect the resulting cell suspension in a 50-mL polypropylene tube and ... that the cell line be maintained in culture and frozen cells tested to ensure that viable uncontaminated cells can be recovered follow- ing the freezing process before the cell li...