... mMdithiothreitol, in a final volume of % 350 lL, and by incubating the resulting solutions for 1 hat 37 °C in the dark. After reduction, 1 lLof4-vinylpyri-dine was added, and the incubation was continued ... bradykinins, caeruleins, bombesins, sauv-agine, opioid peptides (dermorphins and deltorphins),antimicrobial peptides (dermaseptins and adenoregulin), and tryptophylins [7,9–12]. Interest in these natural ... prolines in these positions, but none was aprotease inhibitor and they departed from the canonicalpattern by having extra residues between cysteines and, in some cases, also missing cysteines....