Báo cáo Y học: GNA33 from Neisseria meningitidis serogroup B encodes a membrane-bound lytic transglycosylase (MltA) pdf

... form terminal 1,6-anhydromuramic acid-containing products [10]. By virtue of their ability to cleave the polysaccharide backbone of the peptidoglycan layer, lytic transglycosylases are thought to play a ... were collected and analysed by SDS/PAGE. Blue Dextran 2000 and the molecular-mass standards ribonuclease A, chymotryp- sin A, ovalbumin A, and BSA (Pharmacia) were used to calibrate...

Ngày tải lên: 31/03/2014, 23:20

... (PerkinElmer). Northern blot analysis Total Poly (A) + RNA from < /b> 13- and 33-week-old human fetal cerebrum (Biochain) was electrophoresed in a 1% agarose gel containing 0.66 M formaldehyde and was blotted onto a Hybond-N + nylon ... Zhou 2 , Mark Bartlam 1 , Qing Guo 1 , Yiwei Liu 1 , Yixin Sun 1 , Yu Gao 1,2 , Sheng Ye 1 , Guangtao Li 2 , Zihe Rao 1 , Boqin Qiang 2 and Jiangang Yua...

Ngày tải lên: 08/03/2014, 22:20

... growth factors in gastric tissues in response to the damage by CY and protection by AP. 2. Materials and Methods Chemicals and Reagents All chemicals and reagents were of analytical grade and ... purchased from < /b> Minxian County, Gansu Province, China. Polysaccharides fraction was isolated by the ethanol precipitation method as described by Cho et al [7] and modified by Ye et al....

Ngày tải lên: 02/11/2012, 10:19

... enzyme in a final volume Scheme 1. Biosynthetic pathway leading to clavulanic acid. BLS, beta- lactam synthetase (ORF3); PAH, proclavaminate amidino hydrolase (ORF4); CAS, clavaminate synthase ... Previously it has been repor- ted that the b subunit of OATs from < /b> thermophiles run anomalously by SDS/PAGE analysis [25] and the b subunit of OAT from < /b> yeast has been observed as...

Ngày tải lên: 08/03/2014, 22:20

... acuticeps, has been partially character- ized; it is an H -type homopolymer, as i ndicated by the N-terminal amino-acid sequence, that is able to accumulate iron as an L-rich mammalian ferritin ... w by standard procedures. Analysis of the state of association The state of association was analysed by size- exclusion chromatography experiments at 20 °C on a Superose 12 column (Pharmac ia) .....

Ngày tải lên: 08/03/2014, 23:20

... purchased from < /b> Sigma (Deisenhofen, Germany). Peptide-N 4 -(N-acetyl -b- glucosaminyl)asparagine amidase A from < /b> almond (PNGase A) was from < /b> Seikagaku (Tokyo, Japan) and b- galactosidase from < /b> jack beans was ... [42]. Methylation analysis PA-oligosaccharides were permethylated and hydrolyzed. Partially methylated alditol acetates obtained after sodium borohydride...

Ngày tải lên: 31/03/2014, 08:20

... Hiroshima, Japan; 4 PHP Laboratory for Molecular Biology, Sendai, Japan When the a and b chains were separated from < /b> human oxyhemoglobin (HbO 2 ), each individual chain was o xidized easily to the ... a key role in stabilizing the bound dioxygen Further evidence from < /b> the iron valency hybrids Jun pei Yasuda 1 , Takayuki Ichikawa 1 , Mie Tsuruga 1 , Ariki Matsuoka 2 , Yoshiak...

Ngày tải lên: 31/03/2014, 15:20

... detailed data on particular aspects of a patient's condition and treatment – are accessible via tabs displayed across the top of the summary screen. Patient details have been removed. Available ... triangulate three types of qualitative data: video-based interaction analysis, observation, and interviews. Video-based interaction analysis is a technique intended 'to identify reg...

Ngày tải lên: 25/10/2012, 10:31

... González 3 , Nora Elena Saldarriaga 4 , Marisol Bedoya 1 , Juan Manuel Toro 5 , Jorge Byron Velásquez 4 , Juan Carlos Valencia 4 , Clara Maria Arango 5 , Pablo Henrique Aleman 1 , Esdras Martin Vasquez 4 , ... studies by Van den Berghe and colleagues [5,6], may explain our findings as it is possible that any benefit may only be accrued early on. Severe hypoglycaemia of 40 mg/dl or less was...

Ngày tải lên: 25/10/2012, 10:35

... Anwer 3 , Hammam A. Mowafy 1 , Ibrahim M. El-Bagory 1 , Mohsen A. Bayomi 1,2 and Ibrahim A. Alsarra 1,2,  1. Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, ... AppliChem (Darmstadt, Germany). Polyvinyl alcohol (PVA), M.W. 22,000 was obtained from < /b> BDH Laboratories (Poole, England). All other reagents and chemicals were of analytical gr...

Ngày tải lên: 25/10/2012, 11:22