... 353–729 of G. glabra GgbAS1 b-amyrin synthase [6], was prepared byPCR using GgbAS1 as a template, Taq DNA polymerase(Takara Shuzo, Kyoto, Japan), the primers 50-GAAGCATATCCACTATGAAGATGA-30 and ... Hiraoka, N.,Ikeshiro, Y. , Kushiro, T., Shibuya, M. & Ebizuka, Y. (2001)Cloning and characterization of a cDNA encoding b-amyrinsynthase involved in glycyrrhizin and soyasaponin biosynthesis ... mRNApreceded the accumulation of bryonolic acid. In contrast, the level of cycloartenol synthase mRNAwas high at days 4 and 8 and decreased at later growth stages. Similar results werealso obtained in the...