Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx

... Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening Fikri E. L. Yahyaoui 1 , Chalermchai ... RSB-5¢: 5¢-CAAAGAGCACCCTCATTCCAGCC-3¢,andFSD-3¢: 5¢-AGGAGGCAAGCATAGACTTAACG-3¢; CM-AAT2 was amplified with RSB-5¢ and FSA-3¢:5¢-GATAATT CCACACCCTC...

Ngày tải lên: 31/03/2014, 21:21

... between thedomainsaredepictedby An open box in the nucleotide binding domain indicates the NAD + -binding domain (Gly-Xaa-Gly-Xaa 2 - Gly-Xaa 17 -Asp) and all conserved residues implicated for the NAD + binding ... respectively [14]. These data indicate, by analogy, that serine biosynthesis may also be regulated to maintain the intracellular cysteine concentra- tion in the a...

Ngày tải lên: 19/02/2014, 13:20

... critical evaluation of the manuscript and Barbara Zarzycka (Warsaw) for her technical assistance. This work was supported by an International Center for Genetic Engineering and Biotechnology (ICGEB) ... Bovine S100B, bovine a- lactalbumin and horse skeletal myoglobin (all from Sigma) were used as control protein samples. Myoglobin and a- lactalbumin produce single bands of m...

Ngày tải lên: 22/02/2014, 07:20

... Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase Tadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi and Tadayuki Imanaka Department of Synthetic ... cells, as it is presumed to play a vital role in providing acetyl-CoA and oxaloacetate in the cytosol as starting materials for a variety of biosynthetic pathways. Rat and huma...

Ngày tải lên: 08/03/2014, 22:20

... skeletal muscle (lane 2), intestine (lane 3), lung (lane 4), brain (lane 5), adipose tissue (lane 6), heart (lane 7) and skin (lane 8) homogenates and those of rat liver (lane 9) and intestine (lane ... quenching data were also analyzed by direct fitting to an equation containing an additional linear term, but the value obtained for the association constant was indistin- guisha...

Ngày tải lên: 08/03/2014, 22:20

... sequence obtained was displayed an ORF of 258 bp encoding a polypeptide of 85 amino acids and termed BmKIM. The 5¢ and 3¢ UTRs of BmKIM cDNA are 17 bp and 76 bp, respectively. A single AATAAA polyadenylation ... characterized by minima at 207 nm and by a maximum at 190 nm. The negative band at 207 nm had a lower intensity in the case of rBmKIM in comparis...

Ngày tải lên: 31/03/2014, 09:20

... lipase activity and a normal esterase activity, displays a substantial conformational change in the substrate-binding site in the C-terminal domain (red arrow). Modeling of the K41 4A mutant, which ... N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the subst...

Ngày tải lên: 21/02/2014, 15:20

... showed that Rab31, Rab32 and Rab1 1A were equally enriched in the granule/mitochondrion and membrane fractions and that no R ab31 or R ab32, and only a s mall amount of Rab1 1A, was present in the supernatant (cytosol) ... granules. In the present study, Rab31, Rab32 and Rab1 1A were also found in both granule and membrane fractions, and only R ab1 1A was al...

Ngày tải lên: 08/03/2014, 16:20

... 353–729 of G. glabra GgbAS1 b-amyrin synthase [6], was prepared by PCR using GgbAS1 as a template, Taq DNA polymerase (Takara Shuzo, Kyoto, Japan), the primers 5 0 -GAAGCATA TCCACTATGAAGATGA-3 0 and ... Hiraoka, N., Ikeshiro, Y. , Kushiro, T., Shibuya, M. & Ebizuka, Y. (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin...

Ngày tải lên: 08/03/2014, 23:20

... of competitor double stranded target DNAs used as controls were 5 0 - TAATATGT AAAAACATT-3 0 (sense strand, NF-IL 2A) , 5 0 - CACTTGAT AACAGAAAGTGATAACTCT-3 0 (sense strand, GATA-1) and 5 0 -CATGTTATGCATATTCCTGTA- AGTG-3 0 (sense ... 2001 data using a molecular dynamics approach. These data have underlined that the loss of charged phosphate groups and the different shape of...

Ngày tải lên: 24/03/2014, 04:21