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Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx

Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx

Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx

... Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening Fikri E. L. Yahyaoui1, Chalermchai ... RSB-5¢:5¢-CAAAGAGCACCCTCATTCCAGCC-3¢,andFSD-3¢:5¢-AGGAGGCAAGCATAGACTTAACG-3¢; CM-AAT2was amplified with RSB-5¢ and FSA-3¢:5¢-GATAATTCCACACCCTCCAATTA-3¢; the internal standard wasamplified with act.5¢:5¢-gcactgaagagcatccggtacttc-3¢ ... of Arabidopsis and tobacco; and (b) acyl transferases such asanthranilate N-hydroxy-cinnamoyl/benzoyl-transferase(NHCBT)-like protein of Arabidopsis and Dianthus caryo-phyllus, acetyl-CoA benzyl alcohol...
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Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx

Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx

... betweenthedomainsaredepictedby An open box in the nucleotide bindingdomain indicates the NAD+-binding domain (Gly-Xaa-Gly-Xaa2-Gly-Xaa17-Asp) and all conserved residues implicated for the NAD+binding ... respectively [14]. These dataindicate, by analogy, that serine biosynthesis may also beregulated to maintain the intracellular cysteine concentra-tion in the amoeba. Modulation of expression of PGDH and ... Rosenthal, B. & Samuelson, J. (2000) Early lateraltransfer of genes encoding malic enzyme, acetyl-CoA synthetase and alcohol dehydrogenases from anaerobic prokaryotes toEntamoeba histolytica....
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf

Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf

... critical evaluation of the manuscript and Barbara Zarzycka (Warsaw) for her technical assistance. This work wassupported by an International Center for Genetic Engineering and Biotechnology (ICGEB) ... Bovine S100B, bovine a- lactalbumin and horse skeletal myoglobin (all fromSigma) were used as control protein samples. Myoglobin and a- lactalbumin produce single bands of monomericweight and ... has allowed us to analyze the biochemical and structural properties of CR I–II and tocompare them with Calb I–II. Our data show that CR I–II and Calb I –II share virtually the same EF-hand structures.However,...
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Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt

Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt

... Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyaseTadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi and Tadayuki ImanakaDepartment of Synthetic ... cells, as it ispresumed to play a vital role in providing acetyl-CoA and oxaloacetate in the cytosol as starting materials for a variety of biosynthetic pathways. Rat and human ACLs fromvarious ... in lanes 2 and 5, and AclB subunits (b) in lanes 3 and 6. Molecular masses (kDa) are indicated on the side of each panel. The asterisk indicates the degradation product of AclA described in the text.Fig....
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx

Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx

... skeletal muscle (lane 2), intestine (lane 3), lung (lane 4),brain (lane 5), adipose tissue (lane 6), heart (lane 7) and skin (lane 8)homogenates and those of rat liver (lane 9) and intestine (lane ... quenching data were also analyzed by direct fitting to an equation containing an additional linear term, but the value obtained for the association constant was indistin-guishable from that obtained ... 7.4).45Ca2+binding was determined by the method of Mani and Kay[77]using15lMapo-(lungfish calhepatin) as describedby Dell’Angelica et al. [10]. Binding data were analyzed bynonlinear regression...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... sequenceobtained was displayed an ORF of 258 bp encoding a polypeptide of 85 amino acids and termed BmKIM. The and 3¢ UTRs of BmKIM cDNA are 17 bp and 76 bp,respectively. A single AATAAA polyadenylation ... characterized by minima at 207 nm and by a maximum at 190 nm. The negative band at 207 nm had a lower intensity in the case of rBmKIM in comparison withthat in the spectra of AaHIT2 (a- toxin) and CssII ... bySDS/PAGE; the fractions containing the recombinantBmKIM protein were then lyophilized.Amino acid composition analysis and N-terminalsequencingAmino acid analysis was carried out essentially as...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... lipase activity and a normalesterase activity, displays a substantial conformationalchange in the substrate-binding site in the C-terminaldomain (red arrow). Modeling of the K41 4A mutant,which ... N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding and catalytic functions in ... domain (40% similarity) and are28% identical in the C-terminal domain (38% similarity). The catalytic efficiency and heparin-binding functions of the N-terminal domain have been extensively studied[13,14]....
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx

Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx

... showed that Rab31, Rab32 and Rab1 1A were equally enriched in the granule/mitochondrion and membrane fractions and that no R ab31 or R ab32, and only a s mall amount of Rab1 1A, was present in the supernatant(cytosol) ... granules. In the present study, Rab31,Rab32 and Rab1 1A were also found in both granule and membrane fractions, and only R ab1 1A was also present in the cytosol. T his observation may be explained ... properties of Rab31 and Rab32New blood platelet Rab proteinsXiankun Bao1, Andrea E. Faris2, Elliott K. Jang1 and Richard J. Haslam1,2Departments of 1Pathology and Molecular Medicine and 2Biochemistry,...
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt

Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt

... 353–729 of G. glabra GgbAS1 b-amyrin synthase [6], was prepared byPCR using GgbAS1 as a template, Taq DNA polymerase(Takara Shuzo, Kyoto, Japan), the primers 50-GAAGCATATCCACTATGAAGATGA-30 and ... Hiraoka, N.,Ikeshiro, Y. , Kushiro, T., Shibuya, M. & Ebizuka, Y. (2001)Cloning and characterization of a cDNA encoding b-amyrinsynthase involved in glycyrrhizin and soyasaponin biosynthesis ... mRNApreceded the accumulation of bryonolic acid. In contrast, the level of cycloartenol synthase mRNAwas high at days 4 and 8 and decreased at later growth stages. Similar results werealso obtained in the...
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Báo cáo Y học: Molecular interactions between nuclear factor kB (NF-kB) transcription factors and a PNA– DNA chimera mimicking NF-kB binding sites doc

Báo cáo Y học: Molecular interactions between nuclear factor kB (NF-kB) transcription factors and a PNA– DNA chimera mimicking NF-kB binding sites doc

... of competitordouble stranded target DNAs used as controls were 50-TAATATGT AAAAACATT-30(sense strand, NF-IL 2A) , 50-CACTTGAT AACAGAAAGTGATAACTCT-30(sensestrand, GATA-1) and 50-CATGTTATGCATATTCCTGTA-AGTG-30(sense ... 2001data using a molecular dynamics approach. These data haveunderlined that the loss of charged phosphate groups and the different shape of helices in PNA–DNA and PNA–PNAhybrids drastically reduce ... speculated that adding PNA units at both termini of the DNA strands causes a distortion in the double strand. The rigid junction between the PNA and the DNA at the 50end of the DNA might play an...
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