Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx
Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx
... Research Council of Canada, Ottawa, Ontario, Canada Structural analysis of the lipopolysaccharide (LPS) of nontypeable Haemophilus influenzae strain 1003 has beenachieved by the application o ... E.K.H. (2001) A new structural type for Haemophilus influenzae li popolysaccharide. Structural a nalysis o f the lipopolysaccharide from nontypeable Haemophilus influenzae strain 486. Eur. J. Biochem. ... Ma˚nsson et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 Martin Ma˚nsson1, Derek W. Hood2, Jianjun...
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... the chemical structure of the innercore-region of the E. coli R4 core-type was hithertounknown. The chemical structure of the outer core region of the latter was determined by methylation analysis ... chemical analysis of 7-O-(2-amino-2-deoxy-a-D-glucopyranosyl-L-glycero-D-manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epimerase less mutant J-5 of Escherichia ... oligosaccharide can be either dueto the lack of the responsible GlcpN-transferase or by the lack of a phosphatase if the phosphate has to be removedprior to glycosylation. The reactivity of E. coli 2513...
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... used.RESULTS The enzymatic activity and molecular stability of the mutants The catalytic activity and the molecular stability of the mutants were assayed because a change in either of these parameters ... Thus, it is the cleavage of the interdomain loop and not of the autolysis orother loops that determines the half-life of chymotrypsinactivity.Keywords: autolysis; inactivation; chymotrypsin; cleavagesite ... seen from their inactivation rates in the presence of equimolaramounts of trypsin (Table 2). The cleavage of the interdomain and autolysis loopsTo follow the autolysis of D-chymotrypsin and the...
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Báo cáo Y học: Structural analysis of Francisella tularensis lipopolysaccharide potx
... inflammogenicactivity of the intact molecule [6]. The presence of longer(compared to the highly endotoxic lipid of E. coli)chainfatty acids, the absence of phosphates and of an acyl groupat O-3 of a-GlcN ... 173.9 (C)p.p.m. can only be explained as resulting from the acylation of A3withacyl3. O-Acylation of GlcN A at O-3 and of acyl2at O-3 agreed with the low-field position of the correspond-ing ... form. Acyl1,acyl2and acyl3residues had hydroxy or acyloxy groups at C-3 (13C signals of C-3 at 69.0–72.4 p.p.m.), while acyl4had no substituents. The signals of acyl chains could only be...
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Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx
... to the nucleotide sequence of the first FXREFig. 3. Deletional analysis of the rat Bsep promoter activity in trans-fected HepG2 cells. Varying lengths of the 5¢ region of the Bsep gene, from ... Thus, we present the nucleotide sequence, as well as a systematic structural and functional analysis of the 5¢ flanking region of the Bsepgene. The 5¢ deletional analysis of the Bsep promoter ... even below that of the wild-typep-126 Luc. Analysis of the binding affinity of the putativeFXRE site for the orphan nuclear receptor FXRTo characterize the FXRE site identified in the Bseppromoter...
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Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
... spectrometry for the analysis of monosulfatedoligosaccharides. Carbohydr Res 304, 1–9.32 Wheeler SF & Harvey DJ (2001) Extension of the in-gel release method for structural analysis of neutraland ... neutraland sialylated N-linked glycans to the analysis of sul-fated glycans: application to the glycans from bovinethyroid-stimulating hormone. Anal Biochem 296,92–100.33 Fukuyama Y, Ciancia ... (2005) 3803–3815 ª 2005 FEBS 3815 Structural analysis of the N-glycans of the major cysteineproteinase of Trypanosoma cruziIdentification of sulfated high-mannose type oligosaccharidesMariana Barboza1,...
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Báo cáo Y học: Structural determination of the O-chain polysaccharide from Agrobacterium tumefaciens, strain DSM 30205 pot
... ionizingcurrent of 0.2 mA.Glycosyl-linkage analysis of LPS, was performed accord-ing to the procedure of Sandford [10]. The permethylated lipopolysaccharide was recovered in the organic layer of the water/chloroform ... components of the external membrane of the bacterium, both the proteins and the lipopolysaccharides (LPS) [3]. In the lattercase, the interaction is based on the recognition of a portion of the lipopolysaccharide, ... °Cfor2h)inordertohydrolyse the Kdo linkage without effecting the acid-labilefuranosidic unit.Combining the information from the analysis of the COSY and NOESY spectra (Fig. 3) and HSQC, the complete...
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Báo cáo khoa học: Structural analysis of the jacalin-related lectin MornigaM from the black mulberry (Morus nigra) in complex with mannose ppt
... and 2, is strongly restricted by the side chain of Lys106 which protrudes from loop 2to close up the cavity. These structural discrepancies of the carbohydrate-binding cavity should have a pro-found ... gJRL. The size and shape of the carbohydrate-binding site of MornigaM and other JRL essentially depends onboth the conformation of the three loops that delineate the binding cavity and the presence ... structure of the binding sites indicates that specificity of the Mora-ceae lectins is primarily determined by the orientation of the side chains of the four amino acid residues resi-dues that form the...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... Blanche F (1995) Purification of the two-enzyme sys-tem catalyzing the oxidation of the d-proline residue of pristinamycin IIB during the last step of pristinamycinIIA biosynthesis. J Bacteriol 177, ... (2000) Func-tional analysis of the small component of the 4-hydrox-yphenylacetate 3-monooxygenase of Escherichia coli W:a prototype of a new flavin:NAD(P)H reductase sub-family. J Bacteriol 182, ... effects of the copy number of the gene and the translational initiation signals. J Biotechnol 32, 139–148.32 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of the head of bacteriophage...
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Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae pot
... Schweda, E.K.H. (2001) A new structural type for Haemophilus influenzae lipopolysaccharide. Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 486. Eur. J. Biochem. ... HepHepb|Hex2-Hex-Hep-AnKdo-ol|Hep|HepaMajor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS2experiments).bMinor isomer of the Hex3 glycoform (estimated from the intensity of the fragments inMS2experiments).Fig. ... Hep3-glycoform (HexNAc1Hex4) of nontypeable Haemophilus influenzae (NTHi) strain 981.Fig. 8. Structure proposed for a Hep4-glycoform (Hex5) of nontypeable Haemophilus influenzae (NTHi) strain...
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