Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx

... Research Council of Canada, Ottawa, Ontario, Canada Structural analysis of the lipopolysaccharide (LPS) of nontypeable Haemophilus influenzae strain 1003 has been achieved by the application o ... E.K.H. (2001) A new structural type for Haemophilus influenzae li popolysaccharide. Structural a nalysis o f the lipopolysaccharide from nontypeable Haemophi...

Ngày tải lên: 31/03/2014, 21:21

... the chemical structure of the inner core-region of the E. coli R4 core-type was hitherto unknown. The chemical structure of the outer core region of the latter was determined by methylation analysis ... chemical analysis of 7-O-(2-amino-2-deoxy-a- D -glucopyranosyl- L -glycero- D -manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose...

Ngày tải lên: 08/03/2014, 09:20

... used. RESULTS The enzymatic activity and molecular stability of the mutants The catalytic activity and the molecular stability of the mutants were assayed because a change in either of these parameters ... Thus, it is the cleavage of the interdomain loop and not of the autolysis or other loops that determines the half-life of chymotrypsin activity. Keywords: auto...

Ngày tải lên: 22/02/2014, 07:20

... inflammogenic activity of the intact molecule [6]. The presence of longer (compared to the highly endotoxic lipid of E. coli)chain fatty acids, the absence of phosphates and of an acyl group at O-3 of a-GlcN ... 173.9 (C) p.p.m. can only be explained as resulting from the acylation of A3withacyl 3 . O-Acylation of GlcN A at O-3 and of acyl 2 at O-3 agreed with th...

Ngày tải lên: 08/03/2014, 09:20

... to the nucleotide sequence of the first FXRE Fig. 3. Deletional analysis of the rat Bsep promoter activity in trans- fected HepG2 cells. Varying lengths of the 5¢ region of the Bsep gene, from ... Thus, we present the nucleotide sequence, as well as a systematic structural and functional analysis of the 5¢ flanking region of the Bsep gene. The 5¢ deletional...

Ngày tải lên: 17/03/2014, 23:20

... spectrometry for the analysis of monosulfated oligosaccharides. Carbohydr Res 304, 1–9. 32 Wheeler SF & Harvey DJ (2001) Extension of the in-gel release method for structural analysis of neutral and ... neutral and sialylated N-linked glycans to the analysis of sul- fated glycans: application to the glycans from bovine thyroid-stimulating hormone. Anal Biochem 29...

Ngày tải lên: 23/03/2014, 15:20

... ionizing current of 0.2 mA. Glycosyl-linkage analysis of LPS, was performed accord- ing to the procedure of Sandford [10]. The permethylated lipopolysaccharide was recovered in the organic layer of the water/chloroform ... components of the external membrane of the bacterium, both the proteins and the lipopolysaccharides (LPS) [3]. In the latter case, the i...

Ngày tải lên: 24/03/2014, 04:21

... and 2, is strongly restricted by the side chain of Lys106 which protrudes from loop 2 to close up the cavity. These structural discrepancies of the carbohydrate-binding cavity should have a pro- found ... gJRL. The size and shape of the carbohydrate-binding site of MornigaM and other JRL essentially depends on both the conformation of the three loops that delineate...

Ngày tải lên: 30/03/2014, 20:20

... Blanche F (1995) Purification of the two-enzyme sys- tem catalyzing the oxidation of the d-proline residue of pristinamycin IIB during the last step of pristinamycin IIA biosynthesis. J Bacteriol 177, ... (2000) Func- tional analysis of the small component of the 4-hydrox- yphenylacetate 3-monooxygenase of Escherichia coli W: a prototype of a new flavin:NAD(P)H red...

Ngày tải lên: 07/03/2014, 02:20

... Schweda, E.K.H. (2001) A new structural type for Haemophilus influenzae lipopolysaccharide. Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 486. Eur. J. Biochem. ... Hep Hep b | Hex 2 -Hex-Hep-AnKdo-ol | Hep | Hep a Major isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS 2 experim...

Ngày tải lên: 08/03/2014, 02:21