Báo cáo khoa học: The soluble form of the membrane-bound transferrin homologue, melanotransferrin, inefﬁciently donates iron to cells via nonspeciﬁc internalization and degradation of the protein pdf

... 4445 The soluble form of the membrane-bound transferrin homologue, melanotransferrin, inefﬁciently donates iron to cells via nonspeciﬁc internalization and degradation of the protein Michael R. ... (MTf2) and a novel protein isoform:Explanation for the membrane-bound and soluble forms of mel-anotransferrin? FEBS Lett. 512, 350–352.22. Richardson, D.R. & Baker, E. (1990) The uptake of iron ... intracellularbreakdown of the 125I-labelled protein and efﬂux of protein- free125I. The release of 125I-Tf and 125I-sMTf from melanoma cells To examine whether sMTf could be internalized and thenreleased...

Báo cáo khoa học: A truncated form of DNA topoisomerase IIb associates with the mtDNA genome in mammalian mitochondria doc

... many proteins impor-ted into mitochondria, the pI of the mitochondrial form of the protein is higher than that of the cytosolic protein form [65,66].It will be surprising if the mitochondrial form ... the a-andb-isoforms arelikely what makes this possible. Consequently, truncation of the C-terminal domain may be essential for the b isoform to be targeted to the mitochondrion and to assume a ... each of which is an essentialcomponent of one of the enzyme complexes of the respiratory chain [2]. Consequently, all of the enzymes and DNA binding proteins required for the replication of mtDNA...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... independently of Pbx, and does notpossess the autoinhibitory function of the Hth domain.In summary, our data suggest that one function of the conserved Hth domain is to inhibit the activity of the transcriptional ... the alternative splice site in Meis3,such that both the Meis3.1 and Meis3.2 isoforms were ampliﬁed. The relative amount of each splice form as a percentage of the totalMeis3 is plotted in the ... wild-typeMeis2d. Together, these data suggest that the Meis3.2splice variant produces a protein that is unable to inter-act with Pbx1, but is also relieved of the autoinhibitoryeffect of the Hth domain.DiscussionWe...

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... side of the protein, indicating apossible additional binding site needed for the WAF1promoter.Prediction of the severity of mutants in otherproteinsAll parameters used for the predictions of ... promoter is measured. The top half shows mutants in which the activity for the p53R2 and NOXA promoters is similar to that of the wild-type, whereas the activity for all the other promoters mea-sured ... Among the 2314 mutants, 1148 were localized in the central coredomain of the protein and were used for training and evalua-tion of our prediction algorithm. Of the eight promoters, westudied the...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity of the pseudouridine synthase RluD in Escherichia coli doc

... obtained with the transition at posi-tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the othertwo ... belongs to the RluA family [2]. Binding of RluA to one of its substrates, tRNAPheanticodonstem-loop, induces reorganization of the RNA [21]. Anability of the RNA substrate to adopt the alternativefold ... (Fig. 4). The presence of pseudouridines in70S but not in free 50S subunits can be explained byreduced rates of pseudouridine formation due to the transition of A1916 to G. The effects of the mutationsin...

Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx

... ligand of ERs, does not bind to any members of the ERR fam-ily [14,19]. Likewise, BPA binds only weakly to ERs and does not bind at all to any other receptors of the ERR family.BPA has the ... the cells were trea-ted with a vehicle solution to measure the basal con-stitutive activity of each receptor, by using exactly the same of amount of expression plasmid of the receptor.Furthermore, ... major role of Arg316 in arresting the ligandWhen the amino acid sequences of the LBD of all the NRs were aligned to that of ERRc, it became notice-able that 26 receptors among the total 48 NRs...

Tài liệu Báo cáo khoa học: N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity ppt

... to the right and the addition of further residues at the N-terminus or beyond residue 34 of the inhibitorysegment is superﬂuous. However, in the case of PpPr, the serendipitous positioning of ... polypeptide. The beneﬁt of these alterations at each extremity of the polypeptidechain, must of necessity arise from contacts that aremade outwith the active site of the enzyme. The effect of removal of ... at the N-terminus of these polypeptides, the potential captureby the residues of the largely negatively chargedsurface adjacent to the edge of the active site cleft(Fig. 3A) may increase the...

Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx

... sensitive to the pres-ence of the anion. These residues also modulate the conformation of unfol-ded cytochrome c, inﬂuencing its spin state and the coordination to the prosthetic group.AbbreviationCT, ... isexpected to be the best candidate for ligand X (the other likely candidates, i.e. the lysines, are fully proto-nated at pH 2.2) [18]. The heterogeneous character of the A-state promp-ted us to investigate ... the HSacid-denatured form, with further formation of the LS(IHH) A-state; (b) for the slow phase, to formation of the LS native-like (IHM) A-state. Unlike wild-typecytochrome c, once the...

Tài liệu Báo cáo khoa học: Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-ﬁn eelpout, Zoarces elongatus Kner ppt

... the SP isoforms when the con-centrations of these isoforms was standardized to 1.0 mm. Signiﬁcantly, the TH activity of the SP isoforms showed concentration dependence in the presence of 0.2 mm ... and lowers the energy barrier to allow adsorption of the ‘less active’ isoform; (b) the less active isoform (SP or QAE2) can then adsorb to the ‘open space’ between the prebound AFPs of the icecrystal ... the SP isoform is generated to reduce the hydrophobicity and improve the solubility of type III AFP isoforms asa whole at the expense of surface complementarity; (b)accordingly, the SP isoform...

Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc

... interms of the number of the enzymes involved, the kineticmechanisms of the competing enzymes and the number aswell as the nature of the allosteric controls. Whether suchdiversity in the organization ... distribution and to what extent changes in the concentration of the inhibitor Pialters the Phser ﬂuxpartition. Due to the numerous interactions in the system, amathematical model of the branch-point ... descrip-tion of the branch-point between the methionine and threonine biosynthesis pathways. In the following the modelis used to investigate the sensitivity of the two-enzymesystem to the different...

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