Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc

... genes encoding fatty acid desaturases involved in eicosapentaenoic acid biosynthesis. Using a combination of PCR, mass sequencing and library screening, the coding sequences of two desaturases ... Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis Fre ´ de ´...

Ngày tải lên: 31/03/2014, 09:20

... d-enantiomers, acting on a variety of d-amino acids, including d-isomers of branched amino acids (Fig. 6). The amino acid residues presumed to be involved in substrate recognition and binding are not conserved ... cloning and functional characterization of a d-amino acid aminotransferase in eukaryotes. The results presented here provide important information for unde...

Ngày tải lên: 30/03/2014, 04:20

... presence of three distinct domains: the rigid CORE-domain and two smaller peripheral domains or mobile parts, the NMP-binding site and the LID-domain [4]. The NMP-binding site has many intermolecular contacts ... from the amino acid sequence of AK2 protein. The purification and functional folding of L. donovani AK from the E. coli cell lysates was carried out by washing and d...

Ngày tải lên: 21/02/2014, 00:20

... 2004 Molecular and functional characterization of novel CRFR1 isoforms from the skin Alexander Pisarchik and Andrzej Slominski Department of Pathology and Laboratory Medicine, University of Tennessee ... exception of an artificial isoform (CRFR1h2) with the insertion of 37 amino acids between the ligand binding domain and the first extracellular loop that was capable of...

Ngày tải lên: 07/03/2014, 15:20

... identified a protein containing a single ankyrin repeat that interacts with HIV-1 viral protein R (Vpr) and we designated this protein as Vpr-binding ankyrin repeat protein (VBARP). This interaction ... ankyrin repeat motif-containing proteins play an important role in protein–protein interactions. ANKHD1 proteins are known to possess multiple ankyrin repeat domains and a single KH domai...

Ngày tải lên: 07/03/2014, 21:20

... (B,C) Actin polymers in the presence of Iba1 (B) and Iba2 (C). (D,E) For comparison, actin polymers are shown in the presence of the actin cross- linking protein neurabin-2 (D) and an actin binding ... reveals functional simi- larities and differences between Iba1 and Iba2. We investigated Ca 2+ binding and homodimerization of Iba1 and Iba2. Furthermore, F-actin bindin...

Ngày tải lên: 16/03/2014, 06:20

... were maintained in RPMI-1640 medium con- taining 10% fetal bovine serum (Invitrogen). All these cells were incubated in a humidified 5% CO 2 incubator at 37 °C. Creation of deletion constructs of ... Protein Engineering, Beijing Institute of Biotechnology, China The poxvirus and zinc finger (POZ) domain, formerly termed the broad complex, tramtrack and bric-a-brac (BTB) domain, wa...

Ngày tải lên: 30/03/2014, 04:20

... and P2Y 1 and P2Y 12 receptor protein expression and functional activity. Examination of the effects of specific pharmacological agents (a single agonist and two specific antagonists of P2Y 1 and ... can induce cell cycle arrest in many cell lines, including astrocytes [26,27]. In the pre- sent study, we used propidium iodide staining and flow ABC DEF GHI Fig. 1. Inductio...

Ngày tải lên: 30/03/2014, 08:20

... molecule involving a-helix A, the nearby loop, the first and last turns of helices E and F, and the C-terminus helices I and J. In addition, there is a lysine-rich N- and C-ter- minus, in accordance ... pylori strains harbouring a func- tional cagPAI, is the activation of nuclear transcription factor-jB and the induction of pro -in ammatory pro- duction of cytokines, ma...

Ngày tải lên: 06/03/2014, 00:21

... PRTFDC1 and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using the ... activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remai...

Ngày tải lên: 15/02/2014, 01:20