Báo cáo khoa học: Domains of ERRcthat mediate homodimerization and interaction with factors stimulating DNA binding potx

... (1994) Nuclear extracts enhance the interaction of fusion proteins containing the DNA- binding domain of the androgen and glucocorticoid receptor with andro-gen and glucocorticoid response elements. ... regulates androgen receptor transcriptionalactivity. J. Biol. Chem. 276, 46647–46654.Ó FEBS 2002 DNA binding of ERRc (Eur. J. Biochem. 269) 4097 Domains of ERRc that mediate homodimerization and interaction with ... analysis of the androgen receptor indicated that that HMG-1 needs atleast part of the CTE and of the hinge region for thestimulation of receptor DNA binding [58]. Whether theobserved effect on DNA...

Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt

... acidiﬁcation of intracellular acidic organ-elles including endosomes, lysosomes, and phagosomeswithout perturbation of the formation of intracellularorganelles and without alteration of the morphology of ... the morphology of vacuolar compartments, resulting in the profoundinhibition of the endosomal ⁄ lysosomal degradation of macromolecules and targeting of lysosomal acid hydro-lases and cholesterol ... 46-kDa and 42-kDa bands are pro-cathepsin E and mature cathep-sin E, respectively.To follow the biosynthesis and processing of wild-type cathepsin E, the transfected cells were labeledwith...

Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc

... kinetic properties of NR1, and as we have shown for CPR and P450 BM3(23,24,28) the properties of the ﬂavin binding domains of NR1 are likely to mimic the redox properties of the domains in full-length ... transferproperties of the FAD- and FMN -binding domains of the humanoxidoreductase NR1Robert D. Finn1, Jaswir Basran2, Olivier Roitel2, C. Roland Wolf1, Andrew W. Munro2, Mark J. I. Paine1 and Nigel ... (CPR). The FAD/NADPH- and FMN -binding domains of NR1 have been expressed and puriﬁed and theirredox properties studied by stopped-ﬂow and steady-statekinetic methods, and by potentiometry....

Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications docx

... membrane- and RNA- binding sites of Era overlap.To further understand the inhibition of liposome on the binding of Era to poly(U), we analyzed the effects of different component lipids of the liposome ... contains the RNA -binding domain (KHdomain) and the region essential for the binding of Era to the cyto-plasmic membrane [20,24].Ó FEBS 2003 16S rRNA- and membrane -binding domains of Era (Eur. ... 24 h and examined for growth.Analysis of the association of the truncated Era proteins of S. pneumoniae with the cytoplasmic membraneTo examine the association of Era, Era-N, and Era-C of S....

Tài liệu Báo cáo khoa học: Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding/unfolding pptx

... intermediates found here with theintermediates found during the refolding studies of HPI, amixture of intermediates P3 and P4 captured during therefolding process were used as a marker. Intermediate ... nativePAGE.Isolation and puriﬁcation of the scrambled disulﬁdeisomers of HPIIn the presence of denaturant and thiol catalyst asindicated above, HPI was converted into the mixture of native and scrambled ... concentration of denaturant.Fig. 3. Unfolding of HPI in the presence of denaturant and thiol cata-lyst. Controls for the disulﬁde scrambling method include the lack of thiol catalyst and lack of denaturant...

Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt

... Therelative abundance of ADH3 within many different tissuetypes is probably related to the need of scavengingformaldehyde for cytoprotection, but low activity of ADH3 with ethanol and retinol cannot ... Tomac,A.,Eriksson,U.,Perlmann,T.&Olson,L.(1999)Roleofretinoids in the CNS: diﬀerential expression of retinoid binding proteins and receptors and evidence for presence of retinoic acid.Eur. J. Neurosci. 11, ... genes ADH1, ADH3 and ADH4.Similar mRNA length and high nucleotide and aminoacid sequence identity of all ADHs lead to a large risk forcross-reactivity of probes at the mRNA and protein level,making...

Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx

... promoteornithine and SAH production, which in turn could beused for the formation of polyamines and methionine.An investigation of the level of different intermediatemetabolites and the expression of different ... prominent up-regulation of the expression and enzymatic activity of both AGAT and GAMT. The speciﬁc activity of AGAT in sarcomatissue reached almost 50% of that observed in thekidney and it was also ... samples and also in theliver, but remained undetectable with this method innormal muscle.RT-PCR and mRNA expression analysis of AGAT and GAMTBoth measurements of catalytic activity and immuno-blot...

Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

... levels observed with TNFa or fMLP alone (Fig. 2B,C) with > 90% of the [3H]PtdBut accumulating within the ﬁrst5 min of stimulation.Effect of PI3-kinase and ARF inhibitors on OÀ2 and PLD activationSelective ... 5B) with an approximate size of 120 kDa. Lysates of CHO cells transfected with human PLD1 were used aspositive controls.The subcellular distribution of PLD and the conse-quences of priming and ... PLD1 as immunoprecipitation with a PLD1-speciﬁc antibody and blotting with the pan-PLD1/2 antibody gave a band of the same density asimmunoprecipitation and blotting with the pan-PLD1/2antibody....

Báo cáo khoa học: Structure of the O polysaccharides and serological classiﬁcation of Pseudomonas syringae pv. porri from genomospecies 4 doc

... namely C3 of RhaII,C2ofRhaIII and RhaIV, and C2 and C3 of RhaIto d 78.5–79.1 (by6–8 p.p.m.), and the similarity of the C2-C6 chemical shifts of the GlcNAc residue to those of nonsubstituted ... whichconsists of four residues of L-Rha and one residue of D-GlcNAc. A relatively large J1,2coupling constant value of 8 Hz for the H1 signal of the GlcNAc residue and theNMR chemical shifts of H5 and ... downﬁelddisplacements of the signals for C3 of RhaI and RhaIV,C2 of RhaIII, C2 and C3 of RhaIIto d 77.3–79.1, i.e. by6–8 p.p.m. as compared with their positions in theTable 2.1H NMR data of O polysaccharides...

Báo cáo khoa học: Isolation of a putative peroxidase, a target for factors controlling foot-formation in the coelenterate hydra docx

... granules, and that it consists of a singlecomponent, the molecular mass of which is in the range of 43–45 kDa. Puriﬁcation of the protein and subsequent clo-ning of its complementary DNA yielded ... areampliﬁcation of an aliquot with 30 cycles and anannealing temperature of 55 °C. From the sequence of thisPCR-fragment new primers were designed and used for thegeneration of the 3¢ and 5¢ ends ... PCRs either with the 3¢ RACE system (GibcoBRL) or with DNA of a(ZAP cDNA library of H. vulgaris as template. For libraryconstruction the mRNA was reverse-transcribed intocDNA and ligated into...

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