Báo cáo Y học: Probing the role of glutamic acid 139 of Anab

Báo cáo Y học: Probing the role of glutamic acid 139 of Anabaena ferredoxin-NADP+ reductase in the interaction with substrates pptx

... Glu139 in FNR substrate interaction (Eur. J. Biochem. 269) 4947 Probing the role of glutamic acid 139 of Anabaena ferredoxin-NADP + reductase in the interaction with substrates Merche Faro 1 , ... [24]. In the present study, further characterization of E139D, E139K and E139Q FNR forms has been carried out in order to elucidate the role of E139 n...

Ngày tải lên : 31/03/2014, 08:20

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Báo cáo Y học: A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor pot

... bond between cysteines C458 and C466 [30]. To analyse the role of the three cysteines in D5 of gp130 in heterodimerization with the LIFR, each of the cysteines was mutated to alanine. Considering the disulﬁde ... spacing and orientation of the cytoplasmic parts of the receptors for signalling. An exchange of gp130 D6 with a domain that does not allow intera...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Probing the rotor subunit interface of the ATP synthase from Ilyobacter tartaricus pptx

... probably occurs as a result of improper folding of the protein, in line with the observed slight deviation of the binding and dissociation kinetics. Binding of the c¢E204A mutant, with or without ... contribute equally to the intrinsic binding energy of complex assembly. The assembly of the c 11 ring and the c¢e complex can be distinguished experimentally by...

Ngày tải lên : 24/03/2014, 00:21

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Tài liệu Báo cáo Y học: Puriﬁcation, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... the activity of the enzyme was measured by the dinitrosalicylic acid method. Inhibition of the enzyme activity by glucose, maltose and cyclohexaamylose For the study of the enzyme inhibition by glucose, ... by the inhibitors of the enzymatic activity. Therefore the iodine staining method is well suited for extensive kinetic analyses of puriﬁed b-amylases. Stabili...

Ngày tải lên : 22/02/2014, 07:20

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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx

... alternate interpretation of the lack of agonist-induced BRET observed in this study is that the translation of agonist binding to the ATDs of the family C GPCR homodimer into G-protein coupling of the ... Agonist-induced rearrangement of the 7TMs in the CaR homodimer? One of the goals of the present study was to investigate, whether the activating twist...

Ngày tải lên : 08/03/2014, 09:20

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Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf

... months without signi®cant loss in activity. The purity of the enzyme was con®rmed by SDS/PAGE with Coomassie-staining of the proteins and by RP-HPLC followed by mass spectrometry [14]. Fig. 1. The ... and the gene encoding the enzyme has been cloned and sequenced. The enzyme was rapidly and irre- versibly inactivated by sodium borohydride o r h ydroxyl- amine in the...

Ngày tải lên : 17/03/2014, 17:20

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Báo cáo Y học: Puriﬁcation, characterization and subunits identiﬁcation of the diol dehydratase of Lactobacillus collinoides pot

... m M for glycerol. The enzyme required the adenosylcobalamin coenzyme for catalytic activity and the K m for the cofactor was 8 l M . Inactivation of the enzyme was observed by both glycerol and cyano- cobalamin. ... equilibrium with 3-hydroxypropionaldehyde formed by the dehydratase from glycerol. This aldehyde can spontaneously form acrolein by thermal dehydration under acid...

Ngày tải lên : 23/03/2014, 21:20

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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx

... analysis of this complex is required. As part of this initial structural study we wanted to conﬁrm the involvement of the C-terminal YopD domain in LcrH binding. For interaction studies with the ... domain of YopD illustrating the hydrophobic and hydrophilic sidedness of the peptide. Thesidechains are displayed for residues 279–295 with the hydrophobic residues c...

Ngày tải lên : 31/03/2014, 21:21

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Báo cáo y học: "CEO- CNE Relationships: Building an Evidence-Base of Chief Nursing Executive Replacement Costs"

... Caring, later augmented by Turkel in the development of the Theory of Relational Complexity [1, 2]. This theory holds that the caring relationship is a self-organizing dynamic process involving ... interchangeablely since some references use Chief Nursing Officer (CNO) as a position title.) Theoretical framework Theoretical frameworks underpinning this study include Maril...

Ngày tải lên : 26/10/2012, 08:57

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Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture"

... reduction of B19 NS1-induced cytotoxicity of liver cells. Our results support the hypothesis that in the setting of persistent B19 infection, direct B19 NS1-induced cytotoxicity may play a role in ... espe- cially within the well-characterized catalytic regions, such as the endonuclease and NTP-binding motifs. The highly integrated functions of complex proteins can...

Ngày tải lên : 26/10/2012, 09:32

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