Báo cáo Y học: A nonphosphorylated 14-3-3 binding motif on e

Báo cáo Y học: A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo pot

... eukaryotic cells by a genetically deﬁned Y. pseudotuberculosis strain and also by several different Pseudomonas aeruginosa strains [34,35]. The Yer- sinia strain expresses and translocates ExoS ... A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo Maria L. Henriksson 1,2 , Matthew S. Francis 2 , Alex Peden 4 , Margareta Aili 2 , K...

Ngày tải lên : 31/03/2014, 08:20

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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx

... lgÆmL )1 CaM in the presence of 0.1 mm CaCl 2 for the PIP Strips TM assay. CaM -binding assay The binding property of CaM was assessed using CaM-aga- rose (Sigma-Aldrich, St Louis, MO, USA). Recombinant PCaP1 ... the N-terminal region, and investigated this. Biochemical analyses, including in vitro myristoylation, demonstrated the N -myristoylation of PCaP1. In addition, PCaP1 has...

Ngày tải lên : 23/03/2014, 07:20

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Tài liệu Báo cáo khoa học: A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 pptx

... suspended in buffer A (10 mm potassium phosphate, pH 7.2 containing 1 mm EDTA) and stored at )30 °C. Enzyme assay and protein determination Enzyme activity was spectrophotometrically assayed as described ... activity as well as l-proline dehydrogenase activity, and functional analysis of each subunit showed that it is the a subunit that catalyzes the dye-NADHDH reaction [3]. W...

Ngày tải lên : 20/02/2014, 01:20

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Báo cáo Y học: The N-terminus of m5C-DNA methyltransferase Msp I is involved in its topoisomerase activity docx

... p18 band was sequenced using an Applied Biosystems automated sequencer. Assay of topoisomerase I activity Topoisomerase I was assayed using CsCl-puriﬁed plasmid pBR322 in topoisomerase assay buffer ... abolition of the topoisomerase I activity while the methyltransferase activity remains intact. Keywords: DNA binding; methyltransferase MspI; proteolysis; topoisomerase I. Methylation of DNA...

Ngày tải lên : 24/03/2014, 00:21

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Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterﬂy, Papilio xuthus docx

... Yokohama City University, Yokohama, Kanagawa; 2 Graduate School of Frontier Biosciences, Osaka University, Toyonaka, Osaka, Japan Retinoid -binding proteins are indispensable for visual cycles in both ... soluble fraction [22,23]. These findings suggest that a visual cycle similartothatintheflyalsoexistsinthebutterflyretina. In addition, it was demonstrated in the Japanese yellow swal...

Ngày tải lên : 17/03/2014, 03:20

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Báo cáo Y học: A b-lysine adenylating enzyme and a b-lysine binding protein involved in poly b-lysine chain assembly in nourseothricin synthesis in Streptomyces noursei pot

... b-lysine binding protein was assayed in a coupled assay with the b-lysine activating enzyme. The assay contained 1±2 pkatal of b-lysine activating enzyme, 0.1 m M b-ly sine, 0.1 lCi [ 3 H]b-lysine, ... amino acids in nonribosomal systems is the covalent activation of amino-acid residues as enzyme-linked t hioesters [12,13]. As the b-lysine activating enzyme is a stand-alone aden...

Ngày tải lên : 17/03/2014, 17:20

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Báo cáo Y học: A new high afﬁnity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor potx

... more potently than the respective singly phosphorylated tyrosines indicating a synergistic eﬀect of these two tyrosine residues with respect to SOCS-3 binding. Surface plasmon resonance analysis, ... 273, 28185–28190. 15. Sasaki, A. , Yasukawa, H., Suzuki, A. , Kamizono, S. , Syoda, T., Kinjyo, I., Sasaki, M., Johnston, J .A. & Yoshimura, A. (1999) Cytokine-inducible SH2 protein-3...

Ngày tải lên : 24/03/2014, 00:21

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Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry ppt

... heparin binding capabilities. Keywords: amyloid P component; heparin binding; surface plasmon resonance; capillary electrophoresis; isothermal titration calorimetry. Amyloid P component (AP) is a heparin -binding ... AP-1 and by incubating the samples in the absence of salt (water instead of phosphate buffer), we obtained less scattered data, as illustrated by the data points (aster...

Ngày tải lên : 31/03/2014, 23:20

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Báo cáo y học: " A retrospective quality assessment of pre-hospital emergency medical documentation in motor vehicle accidents in south-eastern Norway"

... conception and design, acquisition of data and data analysis, and the drafting, revising, and final approval of the manuscript. SS was involved in the study conception and design, data analysis, and ... light-and-siren response for the study purpose only. This study of documentation quality was part of a cross-sectional MVA study evaluating whether patient injury pattern and severity was...

Ngày tải lên : 25/10/2012, 09:56

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Báo cáo y học: "A prospective observational study of the relationship of critical illness associated hyperglycaemia in medical ICU patients and subsequent development of type 2 diabetes"

... groups according to their primary admission diagnosis: i) sepsis (including severe sepsis and septic shock); ii) acute coronary syndrome (myocardial infarction and unstable angina); and iii) all ... patient prone to hyperglycaemia are a subject of speculation, but almost certainly include pre-existing increased insulin resistance and dysfunction of beta cells. Insulin resistance is presen...

Ngày tải lên : 25/10/2012, 10:02

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