Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx

... FEBS 2003 Processing of proNeuropeptide FF A (Eur. J. Biochem. 270) 4199 Identification of proNeuropeptide FF A peptides processed in neuronal and non -neuronal cells and in nervous tissue Elisabeth ... from extracts of non -neuronal COS-7 cells indicating that processing of hproNPFF A at a specific nonbasic residue should be considered to explain the prese...

Ngày tải lên: 31/03/2014, 07:20

... DNA-binding motif in nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation, interacts with the G-quartet forming RNA [22–25]. In addition, ... the guanidini- um group of the Arg in the RGG domain in the C-terminal of EWS. Results and Discussion Several DNA-binding proteins that bind to G-quadru- plex DNA have been...

Ngày tải lên: 15/02/2014, 01:20

... PCR identification using primers b1X and b1B, and the inactivated lpxX mutant was verified by sequencing. Cloning of lpxL and construction of the knockout mutant O35ElpxL A DNA sequence containing ... two mutants in bacterial clearance might reflect differences in the integrity of their outer membrane, binding activity and sensitivity of the murine comple- ment-mediated kil...

Ngày tải lên: 18/02/2014, 14:20

... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin) were differentially expressed in C. ... known to involve major adjustments in the expression of ribosomal genes and genes coding for proteins involved in RNA metabolism and protein synthesis. In fact, protein sy...

Ngày tải lên: 18/02/2014, 16:20

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat 1 ... molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a pro...

Ngày tải lên: 18/02/2014, 17:20

... biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding these enzymes. The biosynthesis of triterpene saponins involves the initial ... Kirakosyan A, Inoue K, Hiraoka N, Ikeshiro Y, Kushiro T, Shibuya M & Ebizuka Y (2001) Cloning and characterization of a cDNA encod- ing b-amyrin synthase involved in...

Ngày tải lên: 19/02/2014, 07:20

... DNA binding, and compared the cDNAs of IFN-treated cells over- expressing intact STAT2 to those of IFN-treated cells overexpressing mutated STAT2 lacking the DNA binding domain. The IFN-inducible expression ... Characterization of the recombinant extracellular domains of human interleukin-20 receptors and their complexes with inter- leukin-19 and interleukin-20. Biochemistry...

Ngày tải lên: 19/02/2014, 07:20

... follows: aprotinin, leu- peptin, pepstatin A, AEBSF and proteinase inhibitor mixture that contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A from Wako Pure Chemical Industries (Osaka, ... 2006 FEBS 627 Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomod...

Ngày tải lên: 19/02/2014, 07:20

... 4.9 a This strain was grown in media lacking leucine. The other strains were grown in media containing leucine. Fig. 5. Complementation of pos5 cell. Indi- cated pos5 and WT cells carrying each gene on ... the poor growth of the pos5 cell on SG solid medium and on and in solid and liquid media lacking arginine (Fig. 5 and Table 6), thereby supporting the partial contribu...

Ngày tải lên: 20/02/2014, 01:20

... boundaries of tACE and ACE2. The zinc prote- ase domain of both tACE and ACE2 is divided into two subdomains [20]. Subdomain I contains the zinc ion and the N-terminus. The C-terminus is found in subdomain ... those residues in subdomain II involved directly in substrate binding (Arg273 in ACE2 and Gln281, Lys511, Tyr520 in tACE, which bind the C-terminal carboxy group...

Ngày tải lên: 20/02/2014, 01:20