Báo cáo khoa học: b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH docx
Báo cáo khoa học: b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH docx
... of Ab aggregation (Eur. J. Biochem. 270) 4293 b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH Genevieve ... aged at pH 5.0 at both timepoints. These results indicate that Ab1–40 oligomers generated at pH 7.4 are more toxic to VSMCs than those generated at pH 5.0.DiscussionThis study demonstrates that ... thatAb aggregates by two separate pathways. One pathway,which is inhibited at pH 5.0 or by metal ions, slowlygenerates stable species that can be measured by PAGE.The other pathway generates unstable...
- 12
- 439
- 0
Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
... Activated PI3K phos-phorylates phosphatidyinositol-4,5-bisphosphate toform PIP3, which further activates Akt and affectsNF-jB activity [31].In the present study, SEC1 -induced translocation ... binds and activates various target genes [36]. During NADPHoxidase activation, phosphorylation of p47phoxallowsthe migration of entire cytosolic complex (p40phox,p47phox and p67phox) ... induces the formation ofROS, and the phosphorylation of IKK-b and IjB-a at 2, 10 and 30 min of stimulation, respectively (Table 3 and Fig. 2E,F). An inhibitor for NADPH oxidaseattenuates the movement...
- 13
- 465
- 0
Báo cáo khoa học: G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis pptx
... generation ofphosphatidylinositol (3,4,5)-trisphosphate from phos-phatidylinositol (4,5)-bisphosphate, a reaction that isreversed by the enzymes phosphatase and tensin homo-logue (PTEN) and ... agonist -induced activation, dopa-mine D2receptors are internalized and form amultiprotein complex that includes b-arrestin, protein phosphatase 2A and Akt. Protein phosphatase 2Ainactivates ... Targets of Akt phosphorylation. Acti-vated (phosphorylated) Akt isoforms are able to regulate key cellular and physiologi-cal processes by phosphorylating a widerange of substrates involved...
- 12
- 392
- 0
Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx
... urea, the protein was purified by cation exchange chromatography in thepresence of urea, and oxidized at low concentrations by airoxygen in the presence of 1 lm CuSO4. The oxidized protein was ... indicates that stable solutions con-taining about 1 mm concentrations of13C,15N-labeled protein were obtained from cultures in minimalmedium; the Protein Data Bank (PDB) entry code and literature ... coli BL21 cells, native mPrP(121–231)was purified to homogeneity by anion exchange chromatog-raphy, hydrophobic chromatography, and gel filtration,with yields of 5–10 mg of pure protein per liter...
- 9
- 458
- 1
Tài liệu Báo cáo khoa học: Multisite protein phosphorylation – from molecular mechanisms to kinetic models pdf
... substrate concentration is so large thatthe enzymes operate near saturation and the kinasereadily dissociates from the phosphorylated target (and, likewise, the phosphatase from the unphosphory-lated ... to dephosphorylation by phosphatases and to competition by moreprocessive substrates.Fig. 8. Integration of signalling events by multisite phosphorylation. (A) Redundance. Phosphorylation at ... transduction networks are formed, in large part, by interacting protein kinases and phosphatases.Phosphorylation of proteins by kinases (or dephosphor-ylation by phosphatases) provides docking...
- 22
- 519
- 0
Tài liệu Báo cáo khoa học: SREBPs: protein interaction and SREBPs Ryuichiro Sato doc
... kinase-3b phosphorylates serine and ⁄ or threonineresidues near the ubiquitination site in SREBP-1 and SREBP-2, and SREBP ubiquitination is enhanced in amanner dependent on the phosphorylation. ... lipogenesis and lipoprotein secretion [15]. This suggests, at least inpart, a mechanism through which dietary saturatedfats stimulate hyperlipidemia and atherogenesis.SREBPs dock on PGC-1b at a domain ... Fbw7 protein when the phosphor-ylation is enhanced by glycogen synthase kinase-3b.SREBPs are modified by another protein, smallubiquitin-like modifier (SUMO). SUMO-1 is a 101amino acid protein...
- 6
- 589
- 1
Tài liệu Báo cáo khoa học: Functional interaction between RNA helicase II⁄Gua and ribosomal protein L4 pptx
... medium was replaced with freshphosphate-free medium containing 40 lCiÆmL)1[32P]ortho-phosphate (Amersham Pharmacia Biosciences, Piscataway,NJ), incubated for 1.5 h and chased with the regulargrowth ... drop-outmedium that lacks tryptophan and leucine indicatesthat these constructs were expressed (Fig. 1B, left).Because protein protein interactions shown by yeasttwo-hybrid system are not always ... upper band remains to bedetermined.Localizations of M2 (amino acids 131–264) and M3(amino acids 131–333) mutants to the nucleolus are inaccordance with the finding that both NLS and NoLSare...
- 15
- 433
- 0
Tài liệu Báo cáo khoa học: Antioxidant protein 2 prevents methemoglobin formation in erythrocyte hemolysates doc
... substrates are alkylhydroperoxides [9], peroxynitrates [13] and hydrogen per-oxides [14], and they detoxify these substrates by oxidationof the Cys at amino acid 47 [9,15]. These proteinsenzymatically ... shown).Preparation of cell lysateBlood from human or mouse was diluted in phosphatebuffered saline (NaCl/Pi), and RBC separated from whiteÓ FEBS 2003 Distribution and function of antioxidant protein 2 (Eur. ... afterRBClysateinnativesamplebufferwaswarmedtoprecipitatehemo-globin. After centrifuging the lysate and the precipitated hemoglobin,10 and 20 lL aliquots of supernatant and the washed and resuspendedhemoglobin pellet were electrophoresed by...
- 8
- 291
- 0
Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
... doi:10.1046/j.1432-1033.2003.03767.xphosphorylated PKB at both Thr308 and Ser473 undercertain conditions [10]. It was also reported that Ser473 ofPKB could be auto-phosphorylated [11], and the phos-phorylation of tyrosine ... phospha-tidylinositide-3,4,5-triphosphate and phosphatidylinositide-3,4-biphosphate bind to the pleckstrin-homology domain ofPKB and recruit PKB to plasma membrane, leading to theactivation ... ofthe above phenotypes were attenuated, and they could notbe significantly stimulated by insulin, indicating that theinsulin effect on metastatic potential was mediated by PI3K and PKB. Only...
- 11
- 615
- 0
Báo cáo khoa học: Adipophilin protein expression in muscle – a possible protective role against insulin resistance pot
... ofpalmitic acid and oleic acid and the ratio between unsat-urated FA and saturated FA is 1.0. The predominantFA in olive oil is oleic acid and the unsaturated ⁄ satu-rated FA ratio is 4.6. ... incorpora-tion in LDs as TAG.Table 2. Unsaturation level and fatty acid composition of the three high-fat diets.Fat source Unsaturated ⁄ saturated fatty acid ratioFatty acid composition (%)16:0 18:0 ... 3-kinase pathway,which is responsible for most of the metabolic actionsof insulin. Upon activation of phosphatidylinositol3-kinase, Akt ⁄ protein kinase B is activated by phosphorylation. Consequently,...
- 13
- 373
- 0
Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015QUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ