Báo cáo khoa học: b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH docx

... of Ab aggregation (Eur. J. Biochem. 270) 4293 b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH Genevieve ... aged at pH 5.0 at both time points. These results indicate that Ab1–40 oligomers generated at pH 7.4 are more toxic to VSMCs than those...

Ngày tải lên: 31/03/2014, 07:20

... Activated PI3K phos- phorylates phosphatidyinositol-4,5-bisphosphate to form PIP3, which further activates Akt and affects NF-jB activity [31]. In the present study, SEC1 -induced translocation ... binds and activates various target genes [36]. During NADPH oxidase activation, phosphorylation of p47 phox allows the migration of entire cytosolic complex (p40 phox , p47 phox and p67 phox...

Ngày tải lên: 19/02/2014, 00:20

... generation of phosphatidylinositol (3,4,5)-trisphosphate from phos- phatidylinositol (4,5)-bisphosphate, a reaction that is reversed by the enzymes phosphatase and tensin homo- logue (PTEN) and ... agonist -induced activation, dopa- mine D 2 receptors are internalized and form a multiprotein complex that includes b-arrestin, protein phosphatase 2A and Akt. Protein phosphata...

Ngày tải lên: 16/03/2014, 05:20

... urea, the protein was purified by cation exchange chromatography in the presence of urea, and oxidized at low concentrations by air oxygen in the presence of 1 lm CuSO 4 . The oxidized protein was ... indicates that stable solutions con- taining about 1 mm concentrations of 13 C, 15 N-labeled protein were obtained from cultures in minimal medium; the Protein Data Bank (PDB) e...

Ngày tải lên: 18/02/2014, 08:20

... substrate concentration is so large that the enzymes operate near saturation and the kinase readily dissociates from the phosphorylated target (and, likewise, the phosphatase from the unphosphory- lated ... to dephosphorylation by phosphatases and to competition by more processive substrates. Fig. 8. Integration of signalling events by multisite phosphorylation. (A) Redundance....

Ngày tải lên: 18/02/2014, 08:20

... kinase-3b phosphorylates serine and ⁄ or threonine residues near the ubiquitination site in SREBP-1 and SREBP-2, and SREBP ubiquitination is enhanced in a manner dependent on the phosphorylation. ... lipogenesis and lipoprotein secretion [15]. This suggests, at least in part, a mechanism through which dietary saturated fats stimulate hyperlipidemia and atherogenesis. SREBPs dock o...

Ngày tải lên: 18/02/2014, 13:20

... medium was replaced with fresh phosphate-free medium containing 40 lCiÆmL )1 [ 32 P]ortho- phosphate (Amersham Pharmacia Biosciences, Piscataway, NJ), incubated for 1.5 h and chased with the regular growth ... drop-out medium that lacks tryptophan and leucine indicates that these constructs were expressed (Fig. 1B, left). Because protein protein interactions shown by yeast two-hybrid...

Ngày tải lên: 20/02/2014, 01:20

... substrates are alkyl hydroperoxides [9], peroxynitrates [13] and hydrogen per- oxides [14], and they detoxify these substrates by oxidation of the Cys at amino acid 47 [9,15]. These proteins enzymatically ... shown). Preparation of cell lysate Blood from human or mouse was diluted in phosphate buffered saline (NaCl/P i ), and RBC separated from white Ó FEBS 2003 Distribution...

Ngày tải lên: 20/02/2014, 23:20

... doi:10.1046/j.1432-1033.2003.03767.x phosphorylated PKB at both Thr308 and Ser473 under certain conditions [10]. It was also reported that Ser473 of PKB could be auto-phosphorylated [11], and the phos- phorylation of tyrosine ... phospha- tidylinositide-3,4,5-triphosphate and phosphatidylinositide- 3,4-biphosphate bind to the pleckstrin-homology domain of PKB and recruit PKB to plas...

Ngày tải lên: 21/02/2014, 00:20

... of palmitic acid and oleic acid and the ratio between unsat- urated FA and saturated FA is 1.0. The predominant FA in olive oil is oleic acid and the unsaturated ⁄ satu- rated FA ratio is 4.6. ... incorpora- tion in LDs as TAG. Table 2. Unsaturation level and fatty acid composition of the three high-fat diets. Fat source Unsaturated ⁄ saturated fatty acid ratio Fatty acid...

Ngày tải lên: 06/03/2014, 09:22