Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications docx

... [19]. Results Effects of the deletion of the RNA- and membrane- binding domains of S. pneumoniae Era on the GTPase activity of the protein To assess the potential effects of removal of the RNA- and membrane-binding ... Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structura...

Ngày tải lên: 31/03/2014, 07:20

... iden- tify the nature of the enzyme and provide additional information about the evolution of the ChEs, including the origins of the acyl pocket, the T exon, and the asymmetric forms of ChE in the vertebrates. Results The ... at the junctions. Given the recent research on the interaction between WAT domains of AChE catalytic subunits and the PRAD...

Ngày tải lên: 18/02/2014, 17:20

... impair or limit the ApoE interaction with these targets. The binding and the consequent shielding of ApoA-I and ApoE by Hpt are expected to influence these apolipoproteins in their interaction not ... worsens the onset and the rate of progression of atherosclerosis. We evaluated the negative effects of Hpt on LCAT activity and cholesterol uptake by hepatocytes by us...

Ngày tải lên: 07/03/2014, 00:20

... characterized by the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues to produce adult tissues (i.e. the development of the gills and the production of ... sequence of about 90 amino acids of unknown function. The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden- tity)...

Ngày tải lên: 19/02/2014, 00:20

... and quantitative real-time RT-PCR, the locations of the primers DN)105 and DN)9 used in ChIP assays, the locations of the primers DN+232 and DN+254 utilized in 5¢-RACE, and the locations of the ... fragments F and I. The number at the right of each RT-PCR product represents the length of the prod- uct. The scheme also shows the location of the r...

Ngày tải lên: 19/02/2014, 06:20

... 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating that the PM H + -ATPase binding site of PPI1 is ... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, P...

Ngày tải lên: 19/02/2014, 07:20

... Presumably, their homologues SNO2, SNO3, SNZ2 and SNZ3 complement the defect. The relationship of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the ... overlapping fragments of chromosome XIII carrying SNO1 and SNZ1 were capable of complementing the defect of mutant K64 (Figs 1 and 2). They are homologues...

Ngày tải lên: 19/02/2014, 12:20

... HEL (C and D; lane 1) and HEK293 (C and D; lane 2) cells. The positions of the molecular size markers (kDa) are indicated to the left and right of the (C) and (D), respectively, whilst the position ... regions of Prm3. The follow- ing lists the identities of the Prm3 gene fragments and cor- responding plasmids generated in either pGL3Basic (pGL3b) or pGL3En...

Ngày tải lên: 19/02/2014, 16:20

... mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (k int ) in the productive complex is independent of the length of the substrate, K m and V max were ... Detailed characterization of the enzyme showed that PelB is highly thermo-active and thermostable, with a melting temperature of 105 °C and a temperature optimum...

Ngày tải lên: 20/02/2014, 03:20

... proteins The I domains of CD11a and CD11b contain binding sites for ICAM-1 and ICAM-2. Further proof for the interaction of ICAM-4 with these I domains was obtained by compar- ing the ability of recombinant ... investigated the effect of divalent cations on the binding of ICAM-4 transfectants to the I domains of CD11a and CD11b and compared the results...

Ngày tải lên: 20/02/2014, 11:20