Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio) pot

... expression, potential cis-acting regulatoryelements of the promoter and gene linkage mapping of the B-FABP gene from zebrafish (Danio rerio). Materials and methods Zebrafish culture and breeding Zebrafish ... including the 5¢ UTR of the Fig. 2. Structure of B-FABP genes from fishes and mammals. (A)Comparison of the exon/intron organization of the zebrafish B-FABP gene (ZF) with the orthologous genes from ... Canada The brain fatty acid-binding protein (B-FABP) is involved inbrain development and adult neurogenesis. We have deter-mined the sequence of the gene encoding the B-FABP in zebrafish. The zebrafish...

... [86]. Nevertheless, the observed homodimerization of the GCD and the metalion dependence of the catalytic activity support the integrity of the expressed proteins.As expected from the high sequence ... motion of the two ECDmonomers from the apo position (blue) to the complex position (orange) [17,36]. (B) Viewed towards the membrane, the juxtamembranedomains in the apo form (blue circles) translate ... lmolÆmin )1 Æmg )1 in the presence of Mn2+, butmuch lower activity (less than 1 %) in the presence of Mg2+[79], as is generally observed for mammalianGCs [85] and ACs [86]. The activity of the...

... energybetween the protein and ligand, van der Waals energybetween the protein and ligand and within the ligand, and internal torsion energy for the ligand. To considerFig. 2. Summary of sequential and ... time for the epitope mapping of mAb 4-B8( 8) ⁄ E9(data not shown).Figure 4 shows (A) the 1D1H-NMR spectrum of peptide SD incubated with mAb 4-B8( 8) ⁄ E9 at a ratio of 100 : 1; and (B) the corresponding ... calculate their STDintensity (Table 3). The integral value of the signal of one of the b protons of Leu3 of minor conformer 1, the largest STD intensity of peptide SD, was much lar-ger than those of...

... hydrophobicity of the localenvironment of Tyr63 and/ or a contribution of the binding of the copper to the thiolates of the protein. A plot of the intensity of the signal at 265 nm against the concentration of ... (200 0) Stoichiometry of complex formation between Copper (I) and the N-terminaldomain of the Menkes protein. Biochemistry 39, 6857–6863.23. Richardson, J.S. (198 1) The anatomy and taxonomy of protein structure. ... spectra of EhCopZ(20 lM;40mMMops/10 mMNaCl, pH 7) in the presence of Cu(I) (from top to bottom) at 0, 2, 4, 6, 8, 10, 12, 16, 20 lM. The insert shows the plot of the intensity of the dichroic...

... cloned i nto the same expression vector under the control of the T7promoter, and coexpression of oraE and oraS genes wascarried out in E. co li. Meanwhile, the extent of the involvement of AdoCbl ... the formyl group of the c ofactor and the aminogroup of a protein residue. In contrast, the absence of absorption maximum at 420 nm of the mutant enzymespectrum directly demonstrates that the ... determined by measuring the apparent Kmvalue of D-ornithine at 25 , 50, 100, 200 and 400 lM of the inhibitor.For the measurement of the activity of the substrateanalogues, an HPLC and NMR-based method...

... s )1 ; Q116H, 0.0068 s )1 and Q116N 0.011 s )1 .In the cases of the di-zinc species (i.e. the wild-type and Q116H), these rather similar apparent kivaluesmight correspond to the loss of the ... (Macclesfield, UK). Imipenem (De300= )9 000 m )1 Æcm )1 ) was a gift from Merck Sharpe and DohmeResearch Laboratories (Rahway, NJ, USA). Benzylpenicillin(De235= )7 75 m )1 Æcm )1 ) was a gift from Rhoˆ ... ne-Poulenc(Paris, France). Chloramphenicol, cefoxitin (De260= )6 600 m )1 Æcm )1 ), cephalothin (De273= )6 300 m )1 Æcm )1 ) and EDTA were purchased from Sigma (St Louis, MO,USA) and nitrocefin (De482=...

... inhibi-tion of (A) mAb BH216 (20 nM)andof(B)mAb BH195 (20 nM ) was assessed by mea-suring RUrelinthepresenceof10lM of monosubstituted oxidized (black bars) and reduced (white bars) competitor ... analogues The HNE peptide was gradually truncated from the N- and the C-termini and the shortened analogues were assessed forbinding of mAb BH216 (Fig. 4). The five first amino acids of the N-terminus ... constants of only 10 )6 )1 0 )7 Mfor anti -protein antibodies. The affinity of the anti-hemagglutinin mAbBH216 for the peptide is unusually high (Kd¼ 7.60 nM ), compared to the maximal values of 0.1...

... ghrelin and adiponectin alter hypothalamic AMPK and malo-nyl-CoA levels [13,23,28,30–33]. The genetic evidencefor the role of AMPK in the hypothalamus is less clearbecause the loss of the AMPKa2 ... the mitochon-dria and undergoes cleavage by cytoplasmic ATP:citrate lyase producing acetyl-CoA – the sole precursor of fatty acids. The initial and committed step of de novo fatty acid synthesis ... malonyl-CoA level in the hypothal-amus correlate closely and rapidly with reciprocalchanges in the levels of the orexigenic and anorecticneuropeptide expression in the hypothalamus [22].Thus...

... describedabove for the BH assay, except that the temperatureranged from 5 to 56 °C. The activation energy (Ea ) was determined from the slope ()Ea⁄ R) of Arrheniusplots of log V (nmol of product ... values of 60 kDa and 120 kDa (Fig. 3B). Similarly, the purified protein BHAb was eluted from Superdex 200 (data not shown)Fig. 2. Effect of camalexin and 3-phenylindole on the activity of BH from ... by the addition of brassinin (1 mm), and the reaction mixtures were thenincubated for an additional 40 min at 23 °C. In addi-tion, the effects of EDTA (1 mm), 2-mercaptoethanol(0.1 mm) and...