Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio) pot

... E.M. & Wright, J.M. (200 3) Struc- ture, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp 7) from zebrafish (Danio rerio). Eur. J. Biochem. 270, 715–725. 25. ... al.(Eur. J. Biochem. 27 0) Ó FEBS 2003 Structure, linkage mapping and expression of the heart-type fatty acid-binding protein...

Ngày tải lên: 31/03/2014, 07:20

... expression, potential cis-acting regulatory elements of the promoter and gene linkage mapping of the B-FABP gene from zebrafish (Danio rerio). Materials and methods Zebrafish culture and breeding Zebrafish ... including the 5¢ UTR of the Fig. 2. Structure of B-FABP genes from fishes and mammals. (A) Comparison of the exon/intron organization o...

Ngày tải lên: 31/03/2014, 07:20

... [86]. Nevertheless, the observed homodimerization of the GCD and the metal ion dependence of the catalytic activity support the integrity of the expressed proteins. As expected from the high sequence ... motion of the two ECD monomers from the apo position (blue) to the complex position (orange) [17,36]. (B) Viewed towards the membrane, the juxtamembrane...

Ngày tải lên: 28/03/2014, 23:20

... energy between the protein and ligand, van der Waals energy between the protein and ligand and within the ligand, and internal torsion energy for the ligand. To consider Fig. 2. Summary of sequential and ... time for the epitope mapping of mAb 4-B8( 8) ⁄ E9 (data not shown). Figure 4 shows (A) the 1D 1 H-NMR spectrum of peptide SD incubated with mAb 4-B8(...

Ngày tải lên: 16/03/2014, 23:20

... hydrophobicity of the local environment of Tyr63 and/ or a contribution of the binding of the copper to the thiolates of the protein. A plot of the intensity of the signal at 265 nm against the concentration of ... (200 0) Stoichiometry of complex formation between Copper (I) and the N-terminal domain of the Menkes protein. Biochemistry 39, 6857...

Ngày tải lên: 23/03/2014, 12:20

... cloned i nto the same expression vector under the control of the T7 promoter, and coexpression of oraE and oraS genes was carried out in E. co li. Meanwhile, the extent of the involvement of AdoCbl ... the formyl group of the c ofactor and the amino group of a protein residue. In contrast, the absence of absorption maximum at 420 nm of the mutant...

Ngày tải lên: 30/03/2014, 15:20

... s )1 ; Q116H, 0.0068 s )1 and Q116N 0.011 s )1 . In the cases of the di-zinc species (i.e. the wild-type and Q116H), these rather similar apparent k i values might correspond to the loss of the ... (Macclesfield, UK). Imipenem (De 300 = )9 000 m )1 Æcm )1 ) was a gift from Merck Sharpe and Dohme Research Laboratories (Rahway, NJ, USA). Benzylpenic...

Ngày tải lên: 14/03/2014, 23:20

... inhibi- tion of (A) mAb BH216 (20 n M )andof(B) mAb BH195 (20 n M ) was assessed by mea- suring RU rel inthepresenceof10l M of monosubstituted oxidized (black bars) and reduced (white bars) competitor ... analogues The HNE peptide was gradually truncated from the N- and the C-termini and the shortened analogues were assessed for binding of mAb BH216 (Fig. 4). The...

Ngày tải lên: 08/03/2014, 08:20

... ghrelin and adiponectin alter hypothalamic AMPK and malo- nyl-CoA levels [13,23,28,30–33]. The genetic evidence for the role of AMPK in the hypothalamus is less clear because the loss of the AMPKa2 ... the mitochon- dria and undergoes cleavage by cytoplasmic ATP: citrate lyase producing acetyl-CoA – the sole precursor of fatty acids. The initial and committed s...

Ngày tải lên: 14/02/2014, 22:20

... described above for the BH assay, except that the temperature ranged from 5 to 56 °C. The activation energy (E a ) was determined from the slope ()E a ⁄ R) of Arrhenius plots of log V (nmol of product ... values of 60 kDa and 120 kDa (Fig. 3B). Similarly, the purified protein BHAb was eluted from Superdex 200 (data not shown) Fig. 2. Effect of camalexin and...

Ngày tải lên: 18/02/2014, 14:20