Báo cáo khoa học: UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity potx

... UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity Cristina Gagyi 1 , Nadia Bucurenci 2 , Ovidiu ... demonstrating the ability of the encoded protein to synthesize UDP [18]. These observations reopened the question of the role played by UMP kinase in the metabolism of B. subtilis, and...

Ngày tải lên: 31/03/2014, 07:20

... E. coli, the System I cytochrome c biogenesis machinery, consisting of some disulfide bond forma- tion (Dsb) and cytochrome c maturation (Ccm) proteins, is responsible for the biogenesis of a wide variety ... including the latter. Heterologous synthesis of PHCP by E. coli The E. coli System I cytochrome c biogenesis machin- ery, consisting of the Dsb and Ccm proteins, is respon...

Ngày tải lên: 06/03/2014, 00:20

... IPTG. The most favorable conditions for the expression of the enzyme were found to be when IPTG was added at a late stage of cellular growth and when the induction was prolonged for 1 6 h. Therefore, ... modifications as disulfides, could represent a limiting step in their pr oduction [38]. Mechanism of the reaction The reaction catalyzed by PNP is reversible. Thermo- dynamica...

Ngày tải lên: 16/03/2014, 18:20

... the logarithm of the molecular mass of the proteins in the y-axis and the elu- tion volumes (V e ) in the x-axis. Comparison of the elution volumes of the P. abyssi and E. coli ATCases with the standard ... inhibition of the activity. This is the maximal inhibition level by UTP Table 4. Effect of nucleotides on the kinetic properties of the ATCase. V max is t...

Ngày tải lên: 16/03/2014, 19:20

... two molecules from free solution to the solvated complex, and the above-mentioned interaction energy is only one of the components of this free energy. Discussion The high expression yield of the procarboxypeptidase ... correct for the effect of the substrate on the formation of the complex EI, the following equation is applied: K i ¼ K iapp /(1 + [S]/K m ), resu...

Ngày tải lên: 17/03/2014, 03:20

... triterpene biosynthesis. However, information on the OSCs from mangrove species is scarce. To obtain information on the molecular structure of OSCs from mangrove plants, it is important to understand the ... multi- functional terpene synthase that was isolated from the roots of this species (KcMS). This finding also appears to support the view that multifunctional trite...

Ngày tải lên: 23/03/2014, 07:20

... the regulation of mitochondrial function and induction of apoptosis. A recent discovery on the effect of novel PKC isoforms on apoptosis is that phospholipid scramblase 3 (PLS3) is phosphorylated by ... to apoptosis The discovery of the involvement of mitochondria in apoptosis makes this organelle the most important key player supporting apoptotic signaling. Apoptosis is...

Ngày tải lên: 23/03/2014, 07:20

... some extraction (x, k) for s in the corpus. The second is totalSeqConf (s|C), which is the sum of the scores of most confident x in each position: totalSeqConf (s|C) =  k max x totalConf (x, k, s|C) (2) The ... as done in our SEQ system. 286 Pattern Example the ORD the fifth the RB ORD the very first the JJS the best the RB JJS the very best the ORD JJS the t...

Ngày tải lên: 23/03/2014, 16:20

... nutritional regulation. Concerning hormonal regulation, a major contribution comes from studies on rodents and concerns the transition from the fetal to the postnatal animal that is characterized by ... regulation ASS activity which leads to arginine synthesis contributes to three major different functions in the adult organism depending on the cell/tissue considered, as...

Ngày tải lên: 31/03/2014, 01:20

... positive control. Protein determination The protein concentration in crude fractions was estimated assuming an absorption of 10 at 280 nm for a 1% solution. For determining the exact concentration of ... protein resisted Edman degradation. For further sequence information on A. gambiae TrxR, we conducted mass spectral analyses of the two bands from the SDS/PAGE gel. To achie...

Ngày tải lên: 31/03/2014, 07:20