Báo cáo khoa học: A possible physiological function and the tertiary structure of a 4-kDa peptide in legumes potx
Báo cáo khoa học: A possible physiological function and the tertiary structure of a 4-kDa peptide in legumes potx
... A possible physiological function and the tertiary structure of a 4-kDa peptide in legumes Toshimasa Yamazaki 1 , Motoko Takaoka 2,3 , Etsuko Katoh 1 , Kazuki Hanada 2 , Masashi Sakita 2 , Kyoko ... similarity in local structures between them. In the case of insulin, residues at the A- chain N-terminus (GlyA1–IleA2–Val1 3A) [24,25], the A- chain C-ter...
Ngày tải lên: 31/03/2014, 07:20
Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... state may be maintained mainly by key amino acids. In this study, two point-mutated proteins each with a single base substitution [alanine for tryptophan (W14 0A) and alanine for lysine (K13 3A) ] and ... lower than both the wild-type protein and mutants E142O and K13 3A. Thermal analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt
... To amplify the DNA frag- ments containing a complete x-5 gliadin gene, oligonucleo- tides, 5 ¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢ and 5¢-CGTTACATTATGCTCCATTGACTAACAACGA TG-3¢, were constructed based ... sequencing kit and the ABI 3100 DNA sequencer (Applied Biosystems, Foster City, CA, USA). Expression and purification of recombinant protein Sense (5¢-ATTTCATATGCAACAACAATTCCCCCAGC...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf
... dividing datasets during 10-fold cross-validation into training and test subsets with the ratio of 9:1 we randomly split the data into training, validation and test sets with the ratio of 8:1:1. ... as the smallest unit of a language when examining the grammatical structure or the meaning of sentences, referred to as syntax and semantics, however, words them- sel...
Ngày tải lên: 20/02/2014, 04:20
Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot
... CAF-1 – a molecular link between recombination and chromatin assembly during meiosis Satomi Ishii* , †, Akiyo Koshiyama*, Fumika N. Hamada, Takayuki Y. Nara, Kazuki Iwabata, Kengo Sakaguchi and ... with a molecular mass of 120 kDa. The highly charged KER (lysine/glutamate/arginine-rich; 242–360 amino acids) and ED (glutamate/aspartate- rich; 522–578 amino acids) domains in CcC...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Transcriptome profiling analysis reveals multiple modulatory effects of Ginkgo biloba extract in the liver of rats on a high-fat diet pdf
... Lu Zhang 1,2 , Jiahu Pan 3 , Guoping Zhao 1,4 and Qinghua Zhang 1,2,4 1 National Engineering Center for Biochip at Shanghai, China 2 State Key Laboratory of Medical Genomics and Shanghai Institute ... solute carrier family 25 member 1 (Slc2 5a1 ) are involved in lipid biosynthesis, and the downregulat- ed genes for acetyl-CoA carboxylase beta (Acacb) and fatty acid-binding prote...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Antimicrobial peptides from hylid and ranin frogs originated from a 150-million-year-old ancestral precursor with a conserved signal peptide but a hypermutable antimicrobial domain pot
... 5¢-ATAACTGGAACAACGTGTGG-3¢ for caerin 1.1, 5¢-CTAAGTGCTCAGCAATGACG-3¢ for caerin 1.11, 5¢-AGCATAACTGGAACGTGGG-3¢ for caerin 1.12, 5¢-CAGCAATAAGTGGAACAACG-3¢ for caerin 1.13, 5¢-GTGTTTAGCAACGGATTTACC-3¢ for caerin 1.14 and ... Tyler, M.J. & Wallace, J.C. (1998) The maculatin peptides from the skin glands of the tree frog Litoria genimaculata: a comparison of the structures...
Ngày tải lên: 23/03/2014, 17:21
Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc
... and amino acid positions (Fig. 2) indicated that zebrafish PrP1 is, as PrP2, a member of the PrP family. Analysis of the N-terminal repeat domain of zebrafish PrP1 and PrP2 The N-terminal domain ... heart at 48 hpf (B) and in the branchial arches (ba) at 3 dpf (D). Transcripts of prp2 and prp3 are detected in the central part of the pectoral fin (pf) and not...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot
... 6399–6407. 38 Mizushina Y, Kamisuki S, Mizuno T, Takemura M, Asahara H, Linn S, Yamaguchi T, Matsukage A, Han- aoka F, Yoshida S et al. (2000) Dehydroaltenusin, a mammalian DNA polymerase a inhibitor. ... BSA and 0.1% gelatine. Antibodies and gold conjugates were diluted in NaCl ⁄ P i containing 0.1% BSA-C (Aurion, Wageningen, the Netherlands). All wash- ing steps were performed...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf
... dimerization activities of dHAND. We first analyzed the effect of phosphorylation on the transcriptional activity of dHAND using reporter assay (Fig. 6A) . AU5- dHAND, -dHAND-Ala-X, -dHAND-Ala, and ... Gal4-dHAND and functioned as a strong transcriptional activator. Gal4-dHAND-Ala-X and Gal4- dHAND-Ala also showed similar results. The transcrip- tional activity of dHAND-A...
Ngày tải lên: 07/03/2014, 16:20