Báo cáo khoa học: TRAF6 and C-SRC induce synergistic AP-1 activation via PI3-kinase–AKT–JNK pathway pptx

... expression. TRAF6 and c-Src induce AP-1 activation independently Then, in order to investigate the mutual dependency of TRAF6 and c-Src in AP-1 activation, we cotransfected c-Src KD with TRAF6 and determined ... coexpression of TRAF6 and c-Src (Fig. 4D). In contrast, levels of c-Jun mRNA was changed little by the overexpression of TRAF6 and/ or c-Src. Thus...

Ngày tải lên: 31/03/2014, 07:20

... from transfer into pots, and the parts above ground were cut every week. The oldest leaves were comparable to the S3 stage as defined [39]. The influence of light and darkness on expression and activity were ... the proteins and the mix- ture was used for two subsequent immunizations of two rabbits according to standard procedures. In western blot trials using d-CDes recombinant protein...

Ngày tải lên: 16/03/2014, 18:20

... sequences (254 and 106 bp) between the NEG- U and NEG-D elements. The distance between the NEG-U and NEG-D elements was 253 and 106 bp in D-254 and D-106, respec- tively. The right-hand panel shows ... and BETA2⁄ E47 was not mediated via SREs but via the E-boxes. Reducing the distance between the two E-boxes abol- ished synergistic activation. Therefore, the synergistic a...

Ngày tải lên: 30/03/2014, 04:20

... acid, arginine catabolism to NO and polyamines NG, aorta endothelial cells Low affinity transporter and arginase share the control of the fluxes through the pathways involving arginine as a precurser of nitric oxide and polyamines ODE ... Carbohydrate, glycolysis Lactococcus lactis Modeling Pi explicitly and regulation of pyruvate kinase (by Pi and FBP), PFK (by PEP) and GAPDH (by N...

Ngày tải lên: 14/02/2014, 14:20

... of AP-1 via cooperative binding, and creates an accessi- ble environment that also enables Sp1 and Runx1 binding [85,86,233]. Both NFAT and AP-1 have the potential to recruit the HATs CBP and ... factors [86,233]. In response to induction of NFAT and AP-1, these two nucleosomes are evicted and replaced by NFAT ⁄ AP-1 ⁄ Sp1 and NFAT⁄ AP-1 ⁄ RUNX1 enhanceosome-like comp...

Ngày tải lên: 14/02/2014, 18:20

... (C) EcR signaling pathway, (D) Hippo signaling pathway, (E) TGF-b signaling pathway, (F) Hh signaling pathway, and (G) Wnt signaling pathway. miRNAs and cell signaling A. Ichimura et al. 1612 FEBS ... tumors and cell lines, miR-17- 5p and miR-20a are induced in a manner that depends on cyclin D1 and repress the expression of cyclin D1. Hence, miR-17-5p ⁄ 20a and cyclin D1 fo...

Ngày tải lên: 14/02/2014, 19:20

... oncogenesis and cancer metastasis, including MYC, CDK4, CDK6 , E2F3, CREB and MET [33,37,41]. Ectopic expression of miR-34 species induces cell-cycle arrest and apopto- sis and suppresses cell growth and ... [20]. Next, one strand (the passenger strand) of the miRNA is removed from the RLC, whereas the other strand (the guide strand) remains in the complex to form a mature RNA-induc...

Ngày tải lên: 14/02/2014, 19:20

... [78,79]. I ⁄ R in the heart induced miR-21 in cardiac fibroblasts in the infracted region. Thus, I ⁄ R-induced miR-21 limits PTEN function and causes activation of the Akt pathway and increa- sed matrix ... hypertrophy-associated calmodulin, MEF2a and GATA4, and attenuates calcium-dependent signaling through the calcineurin-NFAT pathway [49]. miR-133 inhibits hypertrophy through...

Ngày tải lên: 14/02/2014, 19:20

... binding and cleav- age by ribonuclease A and variants at Lys7, Arg10, and Lys66. Biochemistry 37, 12121–12132. 31 Anderson DG, Hammes GG & Walz FG Jr (1968) Binding of phosphate ligands to ... where I i (h) and I(h) are the ith and the mean measurements of the intensity of reflection h, respectively. R cryst = R h |F o ) F c | ⁄ R h F o , where F o and F c are the observed and...

Ngày tải lên: 14/02/2014, 22:20

... mediate interaction in the first activation site (R144, N154, I152) and three in the second (F127, M436 and H428), and found that Ap 4 A and ADP interact with the same site, but the sites for ATP and BPG remain ... cN-II, cN-III, cN-IA and cN-IB, and both cytosolic and mitochondrial 5¢(3¢)-deoxyribonu- cleotidases [5]. Even though all soluble 5 ¢-nucleotidases share the same...

Ngày tải lên: 15/02/2014, 01:20