Báo cáo khoa học: Human acid sphingomyelinase Assignment of the disulfide bond pattern ppt

... in the absence of a C221-C226 disulfide bond. This proves the presence of the C221-C226 disulfide bond. The appearance of the signals of fragments y¢¢16, y¢¢ 18, y¢¢19 and y¢¢20 2 Da below the ... the masses of their reduced forms support the above results. Analysis of the disulfide bonds of haSMase from human placenta In order to confirm that the disu...

Ngày tải lên: 31/03/2014, 07:20

... and regulators of other enzymes in the human nucleotide metabolism (Table S1). The library can then be seen to constitute the specific metabolome of the nucleotide metabolism, which would be the most ... interacts with both O6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups...

Ngày tải lên: 15/02/2014, 01:20

... and phylogeny. How- ever, the nature of the connections between these factors needs to be understood at the level of the pro- tein domain. The global properties of the HTFN topology are partially ... graph of N ¼ 230 interacting human TFs was obtained (Fig. 1). This can be understood as the architecture of the regulatory backbone. It pro- vides a topological view...

Ngày tải lên: 19/02/2014, 07:20

... changed. (a) In the absence of the K328 side chain, a solvent molecule occupies the posi- tion of the K328 N f atom (Fig. 4A). The hydrogen bonds and distances imply that the properties of the solvent ... at N e . The crystal structures of K328 ⁄ K328A–C12 and K328R and the results of the decarboxylation assays of the ‘bulky’ mutants all point to the orient...

Ngày tải lên: 19/02/2014, 08:20

... calculated per mole of Cbl. At thesametime,relationofA 280 to the molar concentra- tion of Cbl in the sample [25] gave the value of e 280 ¼ 61 900Æ M )1 Æcm )1 , which was quite close to the theoretically ... which stabilizes the thermo-labile bond between the cobalt atom of Cbl and the amino group. At that point the matrix can be either used for application or store...

Ngày tải lên: 21/02/2014, 00:20

... fusions) accumulated in the nucleus, suggesting the presence of NLSs. The strength of these signals can be ranked on the basis of nuclear ⁄ cyto- plasmic partitioning of the fusion proteins as HMG1 ... interesting possibility is the regulation of the effects of TFAM by its subcellular targeting. In the mouse and chicken, but not in the human, expression of a s...

Ngày tải lên: 07/03/2014, 05:20

... per- centages of the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cell cycle after treatment with 20 l M or 100 lM ATP for 24 h. The gat- ing used for the quantification of the cells in the G0 ⁄ ... after addition of agonist (Fig. 5B). These results further indicate the involvement of both MAPKs, ERK1 ⁄ 2 and p38, in the signaling pathways, consistent with the...

Ngày tải lên: 07/03/2014, 12:20

... structure of the 17 glutamines present in the wild-type G-CSF. The first column reports the position of the glutamines in the wild-type protein sequence. The second column indicates the percentage of ... in the protein sequence; the overall height of the stack indicates the sequence conservation at that position, and the height of symbols within the stack...

Ngày tải lên: 16/03/2014, 00:20

... expressed as percentage of trypsin activity measured at the beginning of the incubation. (B) Autolysis in the presence of 100 m M NaCl. (C) Effect of Ca 2+ ontherelativerateofautolysisinthe presence (s) ... significantly by the ratio of the two isoforms, under conditions that were typical of the pancreatic juice. This observation suggests that the primary trypsin funct...

Ngày tải lên: 17/03/2014, 03:20

... of residual enzyme activity was used to determine the inhibition constant K i due to the tight binding of the protease by the inhibitor and the possibility of modification of the inhibitor bytheenzyme[32].Thesedatawerefittedtothetheoretical equation ... comprised of exons 9 and 10. Following the thyropin domain is a region enriched for acidic residues. Twelve of the...

Ngày tải lên: 17/03/2014, 10:20