Báo cáo khoa học: Functional characterization of Drosophila melanogaster PERK eukaryotic initiation factor 2a (eIF2a) kinase pdf

... Pomar et al.(Eur. J. Biochem. 270) Ó FEBS 2003 Functional characterization of Drosophila melanogaster PERK eukaryotic initiation factor 2a (eIF2a) kinase Natalia Pomar, Juan J. Berlanga*, Sonsoles ... fraction (Fig. 6A). Fig. 4. DPERK is an eIF2a kinase. (A) Autokinase and eIF2a kinase activities of recombinant DPERK in vitro. Wild type DPERK spe- cifically phosp...

Ngày tải lên: 31/03/2014, 07:20

... in the course of the reaction. The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c. This was vali- dated by adding a total of 2.5 U of commercial SOD ... and acetate. Kinetic characterization of Fe 2+ Bxe_A2876 The activity of Bxe_A2876 in the O 2 -dependent con- version of b-diketones was strictly dependent on the Fe 2+ cofactor...

Ngày tải lên: 18/02/2014, 06:20

... proteins as front-end D4 desaturases. Functional characterization of front-end desaturases Functional characterization was carried out by deter- mining the FA profiles of Saccharomyces cerevisiae transformed ... function of this class of enzymes merely on the basis of sequence homology. Moreover, they indicate that a com- plete pathway for very-long-chain polyunsaturated fatt...

Ngày tải lên: 07/03/2014, 12:20

... proteolytic degradation as a result of disulfide bridges arising from increased cysteine content, or a combination of these factors. It is of interest, in the context of artemin sta- bility, that disulfide ... have arisen by duplica- tion of the other. Of these options, the ubiquitous phylogenetic distribution of ferritin, in contrast to the apparent restricted dispersal of arte...

Ngày tải lên: 16/03/2014, 11:20

... 1.647 nm (confidence interval of )0.5963 to 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28), respectively. These ... placed under the control of four tan- dem repeats of EcREs inserted upstream of a thymi- dine kinase gene minimal promoter. This switch was reported to have high level...

Ngày tải lên: 16/03/2014, 12:20

... amplitude of the fast phase of the l atter represen ted up to 40% of the total amplitude, with a rate constant (k obs ) independent of Pc concentration (data not shown). From the k obs values of this fast ... The construction of the synthetic gene, e xpression c onditions, purification protocol and characterization of the recombinant protein will be pub- lished elsewhere. Mass...

Ngày tải lên: 16/03/2014, 16:20

... Hadzopoulou-Cladaras Department of Genetics, Development and Molecular Biology, Laboratory of Developmental Biology, School of Biology, Aristotle University of Thessaloniki, Greece Hepatocyte nuclear factor- 4a (HNF-4a) ... a transcript- ion factor that belongs to the superfamily of nuclear receptors. Like other members of the family, HNF-4a is characterized by the presence of...

Ngày tải lên: 23/03/2014, 10:21

... (1990) Reg- ulation of the maltose transport system of Escherichia coli by the glucose-specific enzyme III of the phosphoenolpyruvate-sugar phosphotransferase system. Characterization of inducer exclusion- resistant ... of MalFGK 2 (Eur. J. Biochem. 269) 4085 Functional characterization of the maltose ATP-binding-cassette transporter of Salmonella typhimurium by means of...

Ngày tải lên: 31/03/2014, 09:20

... the surface of the protein into the interior, terminating at the active site near to the FAD cofactor. The presence of the long funnel is a charcteristic of a number of fla- voenzymes. The funnel of LGOX ... comparisons of the funnels of LGOX, LAAO, and PAO, and the residues composing two entrances of the funnel of LGOX. (A) Stereo view of funnels of LAAO, PAO, and LGOX....

Ngày tải lên: 23/03/2014, 05:22

... mutation of deoxyribonucleoside kinase FEBS Journal 274 (2007) 1542–1551 ª 2007 The Authors Journal compilation ª 2007 FEBS 1551 Functional studies of active-site mutants from Drosophila melanogaster ... mechanism and binding of substrates was investi- gated for the multisubstrate Drosophila melanogaster deoxyribonucleoside kinase. Mutation of E52 to D, Q and H plus mutat...

Ngày tải lên: 19/02/2014, 02:20