Báo cáo khoa học: Kininogen-derived peptides for investigating the putative vasoactive properties of human cathepsins K and L docx

... tissue (glandular) kallikrein formskallidin from low- and high-molecular weight kininogens(LMWK/HMWK). Their amount is regulated by kininases,which rapidly breakdown kinins to give peptidyl fragments,some ... ratio, 1); lane 7, cathepsin L/ HMWK (molar ratio, 0.25); lane 8,cathepsin L. Fig. 2. Hydrolysis of kininogen-derived peptides and kinins by hK1 and cathepsins B, L and K. (A) Structure of kininogen-derived ... C-BK peptide (20 l Mﬁnal), plus hK1 (2 nM )and cathepsins L (0.2 nM), K (2 nM)andB(2nM).Kallikrein hK1 (5 nM) and cathepsins L (0.5 nM), K (5 nM)andB(5nM) were incubated with BK-peptide(52...

Tài liệu Báo cáo khoa học: Kinetic basis for linking the ﬁrst two enzymes of chlorophyll biosynthesis doc

... layer of complexity to both the regulation of chlorophyll biosynthesis, and the availability of MgP for signalling to the nucleus. Wesuggest that the relative amounts of both Gun4 and CHLH are ... the methyltransferase and mag-nesium chelatase are likely to be crucial in determining the availability of MgP for both signalling [20] and biosynthetic roles in the chloroplast.ResultsChlH ... dramat-ically accelerates the formation and breakdown of an intermediate in the catalytic cycle of ChlM. In light of the profound effect that ChlH has on the methyltransferase catalytic intermediate, the...

Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx

... than the yield of Lys33GP. The molecular mass of Lys3 3K ⁄ F was determined to be3722.0 Da, which is identical to the theoretical value(Table 1).Chemical synthesis of the core peptide Lys16To ... theoreticalvalue (Table 1).Inhibition kinetic analysis of Lys33GP, Lys3 3K ⁄F, and Lys16 peptide The inhibition kinetics of the native MBTI, Lys33GP,Lys3 3K ⁄F and the Lys16 peptide for bovine ... concentrations of substrate, 300 and 600 lm for BApNA, and 50 and 100 lm for BTEE.AcknowledgementsWe thank Z Y. Guo for helpful discussions. We alsowould like to thank J B. Han for her generous...

Tài liệu Báo cáo khoa học: "Different Structures for Evaluating Answers to Complex Questions: Pyramids Won’t Topple, and Neither Will Human Assessors" docx

... nugget precision and nugget recall, where re-call is heavily favored (controlled by the β parame-ter, usually set to three). Nugget recall is calculatedsolely on vital nuggets, while nugget precision ... or multiple nuggets. The ﬁnal F-score for an answer is calculated in the manner described in Figure 1, and the ﬁnal score of a run is the average across the F-scores of all ques-tions. The metric ... and at the run level (second number). The cor-relation between all three variants are about equal at the level of system runs. At the level of individualquestions, the micro- and macro-averaged...

Tài liệu Báo cáo khoa học: "An API for Measuring the Relatedness of Words in Wikipedia" docx

... computer and key-board in the English Wikipedia.provides multilingual support for the English, Ger-man, French and Italian Wikipedias and can be eas-ily extended to other languages4.4 Software ... believethat the extensive usage of such measures derivesalso from the availability of robust and freely avail-able software that allows to compute them (Pedersenet al., 2004, WordNet::Similarity).In ... softwarebundle. The architecture of the API consists of the following modules:1. RDBMS: at the lowest level, the encyclopediacontent is stored in a relational database manage-ment system (e.g. MySQL).2....

Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

... [48]. The hydrophilicity, lack of structure and associated ﬂexibil-ity are essential for the solubilizing role of the exten-sion in the sHsp and complexation of the sHsp withtarget proteins. The ... between the molecular weight markers thyro-globulin (mass of 669 kDa) and apoferritin (mass of 443 kDa) (Fig. 4), with an average molecular mass of 613 ± 185 kDa, as calculated from the standard ... Fernando P, Abdulle R, Mohindra A, Guillemette JG& Heikkila JJ (2002) Mutation or deletion of the C-ter-minal tail affects the function and structure of Xenopuslaevis small heat shock protein,...

Báo cáo khoa học: "A System for Real-time Twitter Sentiment Analysis of 2012 U.S" pdf

... continuously. It offers the public, the media, politicians and scholars a new and timely perspective on the dynamics of the electoral process and public opinion. 1 Introduction Social media platforms ... as well as some random neutral tweets. It also shows the total volume over time and a tag cloud of the most frequent words in the last five minutes across all candidates. Another crucial feature ... statistical sentiment modeling informed by, and contributing to, an understanding of the cultural and political practices at work through the use of Twitter. 3 The System For accuracy and speed,...

Báo cáo khoa học: Emerging tools for real-time label-free detection of interactions on functional protein microarrays ppt

... collec-tions of ASRs cover only a very limited fraction of the proteome and are unlikely to be available for mostmetabolites or drugs. Labeling query molecules for large scale studies can often ... [1–7].Understanding the dynamic nature of these inter-actions will reveal the functional responsibilities of proteins and the circuits in which they operate [8]. The complex milieu of the living cell has ... function of the mass and conformation of both the query molecule and the immobilized protein, the number of query molecules that are bound to the immobilized protein, and the distance of the boundquery...

Báo cáo khoa học: No evidence for a role in signal-transduction of Na+/K+-ATPase interaction with putative endogenous ouabain potx

... novel steroidhormone and the adrenal gland the major place of synthesis of ouabain. However, it is still much debated whetherouabain or OLF really exist in mammalian blood and tissues, and if they ... doi:10.1046/j.1432-1033.2003.03554.xhypothetical cascade of events initiated by ouabain occu-pancy of a small fraction of the sodium pumps. One of themhad advocated the role of EDLF for years [15].Possible pitfalls for a novel ... OLF.Ouabain-sensitive and -insensitive a-isoforms of Na+ /K +-ATPase in ratsAll isoforms of the catalytic a-peptide of Na+ /K +-ATPasehave similar, high afﬁnity for ouabain [17] with K din the order of one...

Báo cáo khoa học: "TWO THEORIES FOR COMPUTING THE LOGICAL FORM OF MASS EXPRESSIONS" doc

... true of unions of elements of the semilattice). These predicates are not only true of the conventional kinds but also of conventional servings such as the bottle of wine on the table or the ... true of individual men and of kinds of men. The s-theory rather has a lexical. extension rule which will convert the lexical count noun man into one which is a mass noun and is true of the ... examples of the application of mass predicates to objects.) Generally speaking, the theories view the kinds of M as forming an upper semilattice of kinds with M at the top. This is a "formal"...

Xem thêm