... nm. The A360values were multiplied by 1000 for construction of the curves. The standard error ranged from 4.2 to 7.0%. (D) Citratesynthase at 150 nM was heated at 43 °C for 1 h in either the ... oligomer size, indicating that p26, and byextrapolation, other polydisperse sHSPs tolerate chargereduction at the dimer–dimer interface. The F112Rmodification, on the other hand, placed two ... structure, and the pronounced changesin variant R114A, as indicated by these physical measurements, correlatedwith the greatest loss of function. Although mutation R114A had the greatest effect...