... transcription factorsthat bind to AP-1 or FIN-1 o r that inactive b inding ortransactivation capacity of, for example, FIN-1 and USF-1,could explain activation or inhibition of FiRE. From ourresults, ... consisted of a denaturating step (at 94 °Cfor1min),an annealing step (at 6 0 °C for 30 s) and an extension step(at 72 °C for 1 min) then a ® nal elongation step (at 72 °C for 10 min) in ROBOCYCLERÒ ... mM).Before RNA extraction, cells were incubated for 24 heither in the absence or in prese nce of FSH, dbcAMP,cholera toxin or bFGF, either in combination with FSH ordbcAMP or cholera toxin and...