Báo cáo khoa học: Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot

... Alignment of the sequences of the second Kunitz-modules of the human and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kunitz-domains of the human and the two fugu WFIKKNRP proteins ... Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein...

Ngày tải lên: 31/03/2014, 01:20

... removed from the final product because it did not deactivate the enzyme. EcAIII. The expression and purification protocol of EcA- III yielded 30 mg of pure protein per 1 L of culture. The protein was ... expression of the ybiK gene responded to the presence of cysteine and to defects in t he cysBgene,andtheybiK knockou t mutation impaired the use of glutathio...

Ngày tải lên: 30/03/2014, 15:20

... introduced. The procedures for production and purification of the mutant protein were the same as those of the wild-type. Protein determination and enzyme assay Protein concentrations were determined by the ... describe the construction, coexpression, and purification of the apo- enzyme of D -ornithine aminomutase, together with the temperature effect o n...

Ngày tải lên: 30/03/2014, 15:20

... step. The relatively low purification yield of the enzyme may be due to several reasons. Copper and several other divalent ions stimulated the activity of the tetrathionate hydrolase and they and other ... Dialysis of the crude enzyme extract precipitated about 20% of the total protein, probably because hydrophobic proteins aggregated in the presence of the h...

Ngày tải lên: 07/03/2014, 14:20

... because of the higher concentrations of pesticides (between 1 gÆL )1 and 2mgÆL )1 , according to the herbicide) and shorter time of exposure (between 8 and 24 h) used by the authors. In another ... for 7 and 21 days [22], and some of these genes were also present in the herbicide SSH lib- raries (including glutamine synthetase and cathepsin). In our experiments, we...

Ngày tải lên: 30/03/2014, 15:20

... classes. The basic idea is to find the best segmentation (that is, the one with the highest score) of the portion of the input text up to the i th word. The base case S i would be for i = 1 and it is the ... point of the extracted dialogue act and the starting point of the paired annotation. We do the same for the ending point and we average the two...

Ngày tải lên: 07/03/2014, 22:20

... cata- lysis (the C-terminal residues of each b-strand and the loops that connect each strand, b n , to the following helix, a n ) from those that stabilize the overall fold (the core b-barrel and the ... untested, and the absence of a robust system somewhat limits the potential for redesigning these proteins. The (ba) 8 -barrel proteins are predominantly cytoplasmic,...

Ngày tải lên: 30/03/2014, 20:20

... chan- ges, some of the dynamical features of the free inhibitor are retained in the complex. Comparison of the free and complexed inhibitor structures revealed that most, but not all, of the observed ... con- tribute to the differences in taxon specificity of SGCI and SGTI. The specificity of the interaction of SGCI and SGTI with proteases can only be ass...

Ngày tải lên: 23/03/2014, 10:21

... suggesting the existence of a third inhibitory site. Univalent cations also influence the activity of enolases. Most of the enolases are inhibited by Na + ,whereasthe effect of K + depends on the source ... xamine whether modification of Cys157 and other P. falciparum- specific residues in the vicinity of the active site leads to irreversible inactivation of Pfen. Com...

Ngày tải lên: 16/03/2014, 18:20

... products, distinct from the liver products, at rates of 7 and 17 pmolÆmin )1 Æmg )1 protein. None of the products were identified. In the study of Snider et al. [11], the rates of production of anandamide oxidation ... (spanning the start codon at the 5¢-end) and an XbaI restriction site (at the 3¢-flanking end of the sequence). The insert of the correct s...

Ngày tải lên: 30/03/2014, 04:20