Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II docx

... The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II Kangcheng ... 4. The unfolding of the 33-kDa protein induced by GdmCl in the presence of different sucrose concentrations. Other conditions as in F...

Ngày tải lên: 31/03/2014, 01:20

... cuticular proteins [35,36]. The abundance of His residues in the basic QH proteins therefore raises the possibility that these proteins play an important role in maintenance of the integrity of the ... individuals [1–3]. The mechanical properties of the cuticle depend on the content of chitin, the microarchitecture of chitin filaments, and the interaction betw...

Ngày tải lên: 07/03/2014, 21:20

... treatment of the collected pools to determine the distribution of sialic acid. Next, we completed online MS ⁄ MS analysis of the major species; some of these species were further analyzed by TOF ⁄ TOF ... protons of GlcNAc, and the H3-equitorial and H3-axial protons of N-glycolylneuraminic acid [23–26]. The 1 H-NMR spectrum of cRBCs within the region of int...

Ngày tải lên: 14/02/2014, 19:20

... many of the reactions of the regenerative limb of Calvin cycle with the addition of transaldolase. The function of this pathway is less clearly defined, and may be more varied than that of the Calvin ... attention to the second of these, and consider the likely outcomes of changing the topology of stromal metabolism in the dark. Thus it is that the goa...

Ngày tải lên: 20/02/2014, 23:20

... USA) at the ÔServeis Cientifico-TecnicsÕ of the University of Barcelona, using the 488 nm line of an argon laser and standard optical emission filters. Percentages of cells at each phase of the cell cycle ... behavior of daunorubicin and WP631, we compared the intracellular accumulation of these com- pounds in Jurkat T cells overtime. We also examined the rate and...

Ngày tải lên: 20/02/2014, 23:20

... that stabilize the binding loop. The hydrophobic core is organized differently in the two molecules either b y a Phe of the ®rst or by a Trp of the third b strand. The natural form of PMP-C is fucosylated ... those of small insect toxins, defensins and some other proteinase inhibitors [15±17]. The c ommon feature is the presence of three disul®de bridges and th...

Ngày tải lên: 08/03/2014, 10:20

... envelope of the bound protein. The percentage of protein bound to RNA was the ratio between the value of bound protein and the total protein present in the sample. The relative percentage of binding ... semiquantitive assessment of the relative levels of toxicity of the different mutants was derived by comparing the number of colonies and growt...

Ngày tải lên: 16/03/2014, 03:20

... Disorders and Stroke to WP. The content is solely the responsibility of the authors and does not necessarily represent the of cial views of the National Institute of Neurological Disorders and Stroke ... spectra of the spinophilin ABD constructs (Fig. 2C), which enables rapid analysis of the overall secondary structure content of proteins. The CD spec- tra...

Ngày tải lên: 16/03/2014, 06:20

... by a much slower phase, which is believed to result from slow release of the cleavage products from the protein. The amplitudes of the initial burst (2.78 and 2.83 n M ) gave a ratio of DNA /protein ... Cl – ions, as well as the inclusion of 0.1 mgÆmL )1 BSA did not increase the stability of the protein and slowed the reaction rate (data not shown). Th...

Ngày tải lên: 17/03/2014, 10:20

... involves the formation of a salt bridge between the a-amino group of the substrate and the carboxylic group of Asp235 and a hydrogen bond to the carbonyl atom of Ser330. The d-aldehyde group of the ... pair of A domains and PCPs and catalyze the formation of the peptide bond between the upstream amino acyl or peptidyl moiety tethered to the phosphop...

Ngày tải lên: 23/03/2014, 13:20