Báo cáo khoa học: Characterization of novel sequence motifs within N- and C-terminal extensions of p26, a small heat shock protein from Artemia franciscana potx
... sequence N-terminal extension G 5¢-GGCACTTAACCCATGGTACATGGACCTTGATATTGAC-3¢ 5¢-GTCAATATCAAGGTCCATGTACCATGGGTTAAGTGCC-3¢ R5¢-GGACCTTGATATTGACGGTCCAGATACC-3¢ 5¢-GGTATCTGGACCGTCAATATCAAGGTCC-3¢ C-terminal ... motifs within N- and C-terminal extensions of p26, a small heat shock protein from Artemia franciscana Yu Sun and Thomas H. MacRae Department of Biol...
Ngày tải lên: 30/03/2014, 20:20
... shrimp, Artemia franciscana. Am Zool 39, 836–847. 43 Crack JA, Mansour M, Sun Y & MacRae TH (2002) Functional analysis of a small heat shock ⁄ a- crystallin protein from Artemia franciscana. Oligomerization ... Y, Mansour M, Crack JA, Gass GL & MacRae TH (2004) Oligomerization, chaperone activity, and nuclear localization of p26, a small heat shock...
Ngày tải lên: 07/03/2014, 12:20
... Diego, CA, USA). U0126 was obtained from LC Laboratories (Woburn, MA, USA). Protein assay dye reagent concen- trate was obtained from Bio-Rad Laboratories (Hercules, CA, USA). Glutathione sepharose ... (recombinant analog) and protease inhibitor cocktail for mammalian tissues (P8340) were obtained from Sigma-Aldrich (St Louis, MO, USA). Wortmannin and rapamycin were obtained from...
Ngày tải lên: 15/03/2014, 11:20
Báo cáo khoa học: Coiled–coil interactions modulate multimerization, mitochondrial binding and kinase activity of myotonic dystrophy protein kinase splice isoforms pptx
... pSGVSVDMPK E(1–375): 5¢-ATA GAATTCATGTCAGCCGAAGTGCG- 3¢ and 5¢-ATT CTCGAGTCAAGTGAGCCGGTCCTCCA- 3¢; pSGVSVDMPK E(1–400): 5¢-ATA GAATTCATGTCA GCCGAAGTGCG-3¢ and 5¢-AAT CTCGAGTCAGAAGG GCAGGCGCAC-3¢; pSGVSVDMPK ... pSGVSVDMPK E(60–375): 5¢-ATA GAATTCAGGCTTAAGGAGGTCCGA-3¢ and 5¢-ATT CTCGAGTCAAGTGAGCCGGTCCTCCA-3¢; pSGVSVD MPK E(340–537): 5¢-ATT GAATTCTTTGGCCTTGATTG GGA-3¢ and 5¢-ATA CTCGAGC...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes ppt
... binding domains, and catalytic mechanism of the prokaryotic enzymes Takashi Tadokoro and Shigenori Kanaya Department of Material and Life Science, Osaka University, Japan Identification of bacterial RNase ... archaeal RNase HI can cleave an RNA–DNA junction. Biochem J 381, 795–802. 18 Ohtani N, Yanagawa H, Tomita M & Itaya M (2004) Cleavage of double-stranded RNA by RNase HI...
Ngày tải lên: 30/03/2014, 02:20
Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... interactor, isoform 1 (PPI1) is a novel interactor of the C-ter- minus of Arabidopsis thaliana plasma membrane H + -ATPase (EC 3.6.3.6) (Morandini P, Valera M, Albumi C, Bonza MC,...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... three sets of primers: first set, A5 1 (5¢- GATGTCACGCAGAGTGAGCAGGTAG-3¢)/TRHR-7 (5¢-GAGACCATACAGAAC-C-3¢); second set, A5 2 (5¢- AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); ... (5¢-ATAATGGATAA CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢)andTRHR2-2 sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/ TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT CACC-3¢),...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
... localization and induction by highlight. Mol Microbiol 69, 231–244. 41 Prado-Cabrero A, Estrada AF, Al-Babili S & Avalos J (2007) Identification and biochemical characterization of a novel carotenoid ... subfamily was identified from Arabidopsis thaliana [26], and was later shown to act as a dioxygenase [27]. Sequence homology then allowed the identification and charct...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Characterization of a novel long-chain acyl-CoA thioesterase from Alcaligenes faecalis docx
... thioesterase was active in and stable to a wide range of temperatures and pH values. Maximum activity was observed at 65 °C and pH 10.5 and varied between 60% and 80% at 25–70 °C and pH 6.5–10 (Figs 5 and ... 2387 Characterization of a novel long-chain acyl-CoA thioesterase from Alcaligenes faecalis Puja Shahi* † , Ish Kumar* ‡ , Ritu Sharma, Shefali Sanger and R...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khóa học: Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus influenzae pptx
... procedures Bacterial strains, growth and LPS extraction Nondisease associated NTHi strains 11 and 16 are isolates from the nasopharynx of infants of 21 and 20 months of age, respectively, and are part of a series ... Sciences, National Research Council of Canada, Ottawa, Ontario, Canada; 3 Molecular Infectious Diseases Group, University of Oxford, Department of Paedi...
Ngày tải lên: 16/03/2014, 16:20