Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc

... (2005) 5217–5229 ª 2005 FEBS 5229 Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle Anna Penelova 1 , Larry ... vari- ations. Results Steady-state levels of cyclin mRNA in the cell cycle In a first series of experiments we determined wheth...

Ngày tải lên: 30/03/2014, 20:20

... diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudor coding sequences were joined to the C-terminal region of the GFP or RFP coding ... transcribed from these constructs in vitro was injected into medaka eggs in the one -cell stage to visualize the localization and to mea- sure the mobility of...

Ngày tải lên: 18/02/2014, 16:20

... proteins shows that each of the domains does contribute to the accumulation in cyto- plasmic foci. Similar to what we found for Lsm8p, there appears to be a hierarchy to the contribution of the ... Sm domains or, in the case of Lsm8p, made point muta- tions in putative RNA-binding residues. We investigated the cellular localization of GFP- tagged versions...

Ngày tải lên: 07/03/2014, 02:20

... dashes. The active site (YTVAK) and the cofactor-binding motifs are marked in bold. The D43, T67 and A69 residues, involved in cofactor specificity, are indicated by asterisks. Flanking the N-terminal ... that the N-terminal segment would be sufficient to target and anchor the protein to the ER membranes. Furthermore, we analyzed the contribution of the C-terminal...

Ngày tải lên: 07/03/2014, 09:20

... corresponding to the nodes in the set V L and the other corre- sponding to the nodes in the set V C . Degree distribution of the nodes in V L : Fig- ure 2 shows the degree distribution of the nodes in ... originated. In this paper, we mainly dealt with the occur- rence principles of the consonants in the invento- ries of the world’s languages....

Ngày tải lên: 08/03/2014, 02:21

... therefore attempted to define further the features in the C-ter- minus of 4E-BP1 that are involved in its binding to raptor. The data for the other truncation mutants shown in Fig. 1C indicate that other regions ... that the N-terminus, containing the RAIP motif, and a more C-terminal region (out- side the final 20 residues, i.e. other than the TOS motif) are involve...

Ngày tải lên: 16/03/2014, 06:20

... in the maturation of the enzymes. Most o f them a ct pleiotropically in the synthesis of all four hydrogenases, in particular in the synthesis and insertion of the metal centre [6,7]. Two of the products ... Klenow enzyme to remove the protruding ends and by cloning into the SmaI r estricted vector pT7-7. I n this w ay the GTG start codon of the hypE ge...

Ngày tải lên: 16/03/2014, 18:20

... by PCR to confirm the presence of the RIP/lectin coding sequence in the tobacco genome. Only those plants that were positive in the PCR analysis were withheld for further analysis. Expression of the ... converted into SNA-II and only 5% in SNA-V, the SNA-V-expressing tobacco plants contained little or no rSNA-II, indicating that in tobacco the precursor of rSNA...

Ngày tải lên: 23/03/2014, 12:20

... Dimerization markedly affects the binding ability of the subunits. Notably, heparin and mannose 6-phosphate binding abilities of PSP-II are abolished, indicating that the corresponding binding sites may be ... (Fig. 1A) along with an increase in ellipticity below 250 nm. These changes reflect the loss of tertiary structure of the protein. Monitoring of the decrease wi...

Ngày tải lên: 23/03/2014, 15:21

... need other factors, apart from their mature amino acid sequence, in order to fold efficiently and rapidly into the native form in vivo. The nature of these factors, whether they are found in their ... [18,19]. The extremely inefficient folding of PCI in vitro [18], together with the presence of the above-mentioned long pro-sequences, suggest a possible involvement of...

Ngày tải lên: 23/03/2014, 21:20