Báo cáo khoa học: Identiﬁcation of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathﬁnder selection pot

... regions of immunoglobulins. J. Mol. Biol. 196,901–907.4506 J. Sui et al.(Eur. J. Biochem. 270) Ó FEBS 2003 Identiﬁcation of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathﬁnder ... selectivity.Keywords: CXCR4; Pathﬁnder selection; phage display;scFv; transferrin receptor. Naı¨ve and nonimmune antibody phage libraries are apowerful tool that enables the selection and identiﬁcation of antibodies ... characterization of selected scFvsshowed that none of them were CXCR4 speciﬁc, although antibodies against CD4 and transferrin receptor (TfR,CD71) were identiﬁed. Some of the limitations of theseselection...

Báo cáo khoa học: Identiﬁcation of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx

... structure, and mech-anism. Microbiol Mol Biol Rev 69, 665–695.16 Golby P, Davies S, Kelly DJ, Guest JR & Andrews SC(1999) Identiﬁcation and characterization of a two-com-ponent sensor-kinase and ... that ATP and ADP alsoinhibited MaeE malic and MaeE and CitM OADactivities. The inhibitory effect of ATP was greaterthan that exerted by ADP (Table 1).The consequences of the addition of substrate ... evaluated by the Michaelis–Mentenequation and non-linear regression. The effects of differentmetals, metabolites and substrate analogues on theenzymatic activities were tested by addition of the...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... Afﬁnity of RGG3 for binding to a G-quadru-plex DNA and RNA.Fig. S2. CD spectra of DNAs and RNAs.Fig. S3. Binding afﬁnity of EWS and RBD toHtelo.Fig. S4. In vitro arginine methylation of RGG3 by PRMT3.Fig. ... (lanes 2 and 4) and 32P-labeled Htelo (lanes 1 and 2) orrHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes2 and 4) and 32P-labeled rHtelo (lanes 1 and 2) or mut rHtelo (lanes3 and ... presence of various amounts of RGG3.The CD spectrum of Htelo, a hybrid (3 + 1) form,showed a strong positive band at 290 nm and a nega-tive band at around 235 nm, whereas the addition of 1ratio...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... and structural analysis of OSs inLOSs of lpxX and lpxL mutantsThe OS portion of each LOS was obtained after mildacid hydrolysis and analyzed for its glycosyl composi-tions and by MALDI-TOF ... of the parentalLOS. The LOS band of revertant O35ElpxX alsoshowed a change from brown to black coloration(Fig. 4, lanes 3 and 5).Composition and MALDI-TOF MS analysis of lipid A in lpxX and ... identiﬁed by digestion with BamHI and SacI as well as by sequence analysis, and named pWlpxX and pWlpxL. pWlpxX and pWlpxL were used to transformO35ElpxX and O35ElpxL competent cells by electropora-tion,...

Tài liệu Báo cáo khoa học: Identiﬁcation of differentially expressed genes of the Paciﬁc oyster Crassostrea gigas exposed to prolonged thermal stress docx

... HSPs(HSP70 and HSP23) were greatly and rapidly upregu-lated in gills but slightly less and later in mantle. Oneinducible and two constitutive isoforms of HSP70 aresynthesized in the gills and mantle ... Expression of most transcripts(mainly heat shock proteins and genes involved in cellular homeostasis)showed a high and rapid increase at 3–7 days of exposure, followed by adecrease at 14 days, and ... consisting of a strongshort- and a moderate long-term response. Moreover,after 7–10 days of exposure, we detected differentialexpression of a number of genes that encode elements of the transcription...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... found to have a molecular mass of 64 kDa, and to containtwo tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a protein involvedin ... observed by gel elec-trophoresis. These substances may be aggregates of proteins and polysaccharides, and may play importantroles in formation of the phases and ⁄ or morphologies of the crystals ... vitro,isexpressed in the early stages of development of theinner ear (48 hpf) and is involved in the seeding and ⁄ or nucleation of the sagitta and lapillus [23]. Onthe other hand, omm-64 may be expressed...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... collected and suspen-ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and reﬂuxed for 2 h. Products were extractedwith 50 mL of hexane. The hexane layer was washed with25 mL of saturated ... range of structural diversity produced by asequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-tial origin of structural diversity of skeletons in their biosynthesis, and ... terminated by the addition of 0.5 mL of 40% potassiumhydroxide solution. After boiling for 5 min, products wereextracted twice with 1 mL of hexane. The hexane extractwas evaporated, acetylated, and...

Tài liệu Báo cáo khoa học: Identiﬁcation of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc

... number of genes in U6A and U6A-2 cells, IFN-treated U6A-VV-II cells showedthe down-regulation of a substantially larger subset of genes. Identiﬁcation and characterization of a subset of ISGF3-independent ... both normal and neoplastic pro-liferation, and modulate innate and adaptive immuneresponses [1,2]. The binding of type I IFNs to theirshared cognate receptor, type I interferon receptor (IFNAR), ... Jurisica2 and Eleanor N. Fish11 Department of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, and Department of Immunology, University of Toronto,...

Tài liệu Báo cáo khoa học: Identiﬁcation of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc

... discussions. Thiswork was supported by Grants-in-Aid from the Minis-try of Education, Culture, Sports, Science and Tech-nology and from the Ministry of Welfare and Labor of Japan.References1 Jones ... clearly showed doublet bands at approximately75 kDa and two minor bands at 95 and 110 kDa. Theconditioned media of HSC-4 and HEK293 cells alsoshowed weak immunoreactive bands at the same posi-tions. ... thepresence of 10 mm CaCl2, as described in Experimentalprocedures. Of the nine cell lines tested, OVISE,Fig. 1. Analysis of noncleaved and cleavedforms of IGFBP-rP1 in conditioned media of eight...

Tài liệu Báo cáo khoa học: Identiﬁcation of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

... 40 nucleotides upstream and downstream of POS5 was ampliﬁed by PCR fromplasmid pCgLEU2 by using primers pos5leu21.6f and pos5leu21.6r and was introduced into BY4 742 and MK933(utr1yef1 YCp-UTR1) ... disruption of each gene was conﬁrmed by PCR [34].Construction of other plasmids and strainsUTR1, POS5 and YEF1 ﬂanking 503 bp, 406 bp and 503 bp upstream of each gene were ampliﬁed by PCR fromthe ... not utilized, thereby indi-cating that Yef1p was an ATP-NAD kinase. KmforNAD and ATP were 1.9 mm and 0.17 mm, respectively.Properties of Yef1p and identiﬁcation of Yef1p and Utr1p as ATP-NADH...

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