Báo cáo khoa học: Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection pot

... regions of immunoglobulins. J. Mol. Biol. 196, 901–907. 4506 J. Sui et al.(Eur. J. Biochem. 270) Ó FEBS 2003 Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder ... selectivity. Keywords: CXCR4; Pathfinder selection; phage display; scFv; transferrin receptor. Naı ¨ ve and nonimmune antibody phage libraries are a powerful...

Ngày tải lên: 30/03/2014, 20:20

... structure, and mech- anism. Microbiol Mol Biol Rev 69, 665–695. 16 Golby P, Davies S, Kelly DJ, Guest JR & Andrews SC (1999) Identification and characterization of a two-com- ponent sensor-kinase and ... that ATP and ADP also inhibited MaeE malic and MaeE and CitM OAD activities. The inhibitory effect of ATP was greater than that exerted by ADP (Table 1). The consequenc...

Ngày tải lên: 22/03/2014, 16:20

... Affinity of RGG3 for binding to a G-quadru- plex DNA and RNA. Fig. S2. CD spectra of DNAs and RNAs. Fig. S3. Binding affinity of EWS and RBD to Htelo. Fig. S4. In vitro arginine methylation of RGG3 by PRMT3. Fig. ... (lanes 2 and 4) and 32 P-labeled Htelo (lanes 1 and 2) or rHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes 2 and 4) and 32 P-labeled rHte...

Ngày tải lên: 15/02/2014, 01:20

... and structural analysis of OSs in LOSs of lpxX and lpxL mutants The OS portion of each LOS was obtained after mild acid hydrolysis and analyzed for its glycosyl composi- tions and by MALDI-TOF ... of the parental LOS. The LOS band of revertant O35ElpxX also showed a change from brown to black coloration (Fig. 4, lanes 3 and 5). Composition and MALDI-TOF MS analysis of...

Ngày tải lên: 18/02/2014, 14:20

... HSPs (HSP70 and HSP23) were greatly and rapidly upregu- lated in gills but slightly less and later in mantle. One inducible and two constitutive isoforms of HSP70 are synthesized in the gills and mantle ... Expression of most transcripts (mainly heat shock proteins and genes involved in cellular homeostasis) showed a high and rapid increase at 3–7 days of exposure, followe...

Ngày tải lên: 18/02/2014, 16:20

... found to have a molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a protein involved in ... observed by gel elec- trophoresis. These substances may be aggregates of proteins and polysaccharides, and may play important roles in formation of the phases an...

Ngày tải lên: 18/02/2014, 17:20

... collected and suspen- ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed for 2 h. Products were extracted with 50 mL of hexane. The hexane layer was washed with 25 mL of saturated ... range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity of sk...

Ngày tải lên: 19/02/2014, 07:20

... number of genes in U6A and U6A-2 cells, IFN-treated U6A-VV-II cells showed the down-regulation of a substantially larger subset of genes. Identification and characterization of a subset of ISGF3-independent ... both normal and neoplastic pro- liferation, and modulate innate and adaptive immune responses [1,2]. The binding of type I IFNs to their shared cognate receptor...

Ngày tải lên: 19/02/2014, 07:20

... discussions. This work was supported by Grants-in-Aid from the Minis- try of Education, Culture, Sports, Science and Tech- nology and from the Ministry of Welfare and Labor of Japan. References 1 Jones ... clearly showed doublet bands at approximately 75 kDa and two minor bands at 95 and 110 kDa. The conditioned media of HSC-4 and HEK293 cells also showed weak immunoreac...

Ngày tải lên: 19/02/2014, 07:20

... 40 nucleotides upstream and downstream of POS5 was amplified by PCR from plasmid pCgLEU2 by using primers pos5leu21.6f and pos5leu21.6r and was introduced into BY4 742 and MK933 (utr1yef1 YCp-UTR1) ... disruption of each gene was confirmed by PCR [34]. Construction of other plasmids and strains UTR1, POS5 and YEF1 flanking 503 bp, 406 bp and 503 bp upstream of each g...

Ngày tải lên: 20/02/2014, 01:20