Báo cáo khoa học: Transphosphatidylation activity of Streptomyces chromofuscus phospholipase D in biomimetic membranes docx
Báo cáo khoa học: Transphosphatidylation activity of Streptomyces chromofuscus phospholipase D in biomimetic membranes docx
... transphosphati-
dylation reaction in the presence of diacylglycerol. In order
to detect bis(PA) production in biomimetic membranes
incubated with S. chromofuscus PLD, we prepared vesi-
cles, containing different molar ... phosphatidylalcohol produced can be
directly hydrolyzed by PLD. We also focused on the surface
pressure dependency of PLD-catalyzed hydrolysis of
phospholi...
Báo cáo khoa học: Functional characterization of ecdysone receptor gene switches in mammalian cells docx
... is composed of N-terminal A ⁄ B
domain, the DNA binding C domain, the D domain
(hinge region), ligand binding E domain, and C-ter-
minal F domain. EcR heterodimerizes with other
Keywords
ChIP assay; ... knockdown experiments using RNAi. Chromatin immuno-
precipitation assays demonstrated that VP16 (activation domain,
AD):GAL4(DNA binding domain, DBD):EcR(ligand binding domain,
LBD) or GAL...
Báo cáo khoa học: Bilayer localization of membrane-active peptides studied in biomimetic vesicles by visible and fluorescence spectroscopies pptx
... Decay of the
fluorescence (538 nm) of NBD-PE dye induced by sodium dithionite
following addition of peptides. Data showing dithionite-induced
quenching of fluorescence emission after addition of ... %CR
(degree of blue–red transition; see the Materials and
methods) induced by increasing the quantity of bound
peptides, i.e. the extent of induced blue–red transitions
affected by th...
Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... penta-EF-hand domain. Previously, we
reported that a region containing a tandem repeat of microtubule-interact-
ing and transport (MIT) domains in calpain 7 interacts with a subset of
endosomal sorting ... C2-like domain at its C-ter-
minus. Catalytic triad residues are indicated
by closed triangles. (B) IST1 has a CHMP-
like domain in its N-terminal half, a Pro-rich
linker in the m...
Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt
... In addition, kinetic analysis had shown
binding of Mg
2+
to this subunit [21]. An integrin I domain,
suggested to exist in the C-terminus of the 70 kDa subunit,
is a metal binding site (MIDAS ... facilitating protein
folding and unfolding, assembly and disassembly of protein
complexes, degradation of protein, replication and tran-
scription of DNA, etc. [11–13]. Here we found th...
Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... catalytic serine, therefore protonation of the
histidine would be expected to block catalysis. Rather,
the appearance of activity under conditions in which the
catalytic histidine is protonated indicates ... of an acidic amino acid in the binding of
excess BTC. Excess-substrate activation for human BuChE
has been attributed to binding of positively charged
substrates, such as...
Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx
... understanding of
sHsp substrate interactions will require not only considera-
tion of substrate binding sites and binding interactions, but
also the dynamics of the sHsp oligomer and the kinetics ... respectively. Residues in the C-terminus in bold correspond to the conserved Basic-X-I/V-Q-I/V motif
identified by de Jong et al. [29]. Underlined residues in the C-terminus of TaHsp...
Báo cáo khoa học: Antimicrobial activity of histones from hemocytes of the Pacific white shrimp ppt
... prote ins. Copp er stained
1D SDS 10–20% Tris/HCl gel of h emocyte l ysate p roteins. Molec ular
markers are indicated on the left in kDa. Histone proteins and
hemocyanin protein bands indicated ... peptides are also derived
from larger proteins by proteolysis. For example, bovine
lactoferricin is derived from lactoferrin, an iron bin ding
protein, by pepsin cleavage of its N-terminus...
Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx
... buffer
and air-dried. Filter-associated radioactivity was determined
by liquid scintillation counter.
In the third method [17], GTPase activity was measured
after purified Ndk (1 lg) was incubated with ... incomplete adjuvant and injected into Swiss albino
mice. Subsequently, three injections of 25 lgeachofNdkin
250 lL of Freund’s incomplete adjuvant were given after an
interval of 1...
Báo cáo khoa học: ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by ppt
... partitioning of
Q-column binding material into forms that bind or fail
to bind to an S-column. Also, mixed purine nucleotides
are tightly bound to the non-S-binding fraction, but
this does not explain ... ‘bending-
up’ and ‘bending-down’ trajectories (arrows) suggesting either a
flattened, non-r54-binding, or a pore-region extruded, r54-binding
conformation, respectively (shapes illustrated...